Final report: The Systems Biology of Protein Acetylation in Fuel-Producing Microorganisms

Technical Report · Tue Nov 27 04:00:00 EST 2018

DOI:https://doi.org/10.2172/1483353· OSTI ID:1483353

Rao, Christopher ^[1]

---

1. Univ. of Illinois at Urbana-Champaign, Champaign County, IL (United States); University of Illinois at Urbana-Champaign

Our goal is to determine how protein acetylation affects metabolism in engineered microorganisms. Lysine acetylation is a common post-translational modification that eukaryotes, archaea, and bacteria employ to regulate protein activity. Multiple studies have recently shown that lysine acetylation predominantly targets metabolic enzymes – in fact, most metabolic enzymes are subject to lysine acetylation. Still, we know very little about lysine acetylation, particularly in the case of bacteria. We hypothesize that bacteria employ lysine acetylation as a global mechanism to regulate metabolism in response to their energy and redox status. Our previous work suggests that lysine acetylation may be an attractive and innovative target for metabolic engineering. We propose to investigate how lysine acetylation affects fuel production in engineered microorganisms. The significance of this work is that it will address a fundamental gap in our understanding of bacterial metabolism and identify new approaches for overcoming the problems associated with the production of advanced biofuels.

• Research Organization: University of Illinois at Urbana-Champaign, Champaign County, IL (United States); Idaho National Engineering Laboratory (INEL), Idaho Falls, ID (United States)
• Sponsoring Organization: USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23). Biological Systems Science Division
• DOE Contract Number: SC0012443
• OSTI ID: 1483353
• Report Number(s): DOE-UIUC-SC--0012443
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
Acetylation