Engineering of B800 bacteriochlorophyll binding site specificity in the Rhodobacter sphaeroides LH2 antenna

Swainsbury, David J. K.; Faries, Kaitlyn M.; Niedzwiedzki, Dariusz M.; Martin, Elizabeth C.; Flinders, Adam J.; Canniffe, Daniel P.; Shen, Gaozhong; Bryant, Donald A.; Kirmaier, Christine; Holten, Dewey; Hunter, C. Neil

doi:10.1016/j.bbabio.2018.11.008

Engineering of B800 bacteriochlorophyll binding site specificity in the Rhodobacter sphaeroides LH2 antenna

Journal Article · Fri Nov 09 04:00:00 EST 2018 · Biochimica et Biophysica Acta - Bioenergetics

DOI:https://doi.org/10.1016/j.bbabio.2018.11.008· OSTI ID:1482755

Swainsbury, David J. K.; Faries, Kaitlyn M.; Niedzwiedzki, Dariusz M.; Martin, Elizabeth C.; Flinders, Adam J.; Canniffe, Daniel P.; ; Bryant, Donald A.; ; ; Hunter, C. Neil

The light-harvesting 2 complex (LH2) of the purple phototrophic bacterium Rhodobacter sphaeroides is a highly efficient, light-harvesting antenna that allows growth under a wide-range of light intensities. In order to expand the spectral range of this antenna complex, we first used a series of competition assays to measure the capacity of the non-native pigments 3-acetyl chlorophyll (Chl) a, Chl d, Chl f or bacteriochlorophyll (BChl) b to replace native BChl a in the B800 binding site of LH2. We then adjusted the B800 site and systematically assessed the binding of non-native pigments. We find that Arg_–10 of the LH2 β polypeptide plays a crucial role in binding specificity, by providing a hydrogen-bond to the 3-acetyl group of native and non-native pigments. Reconstituted LH2 complexes harbouring the series of (B)Chls were examined by transient absorption and steady-state fluorescence spectroscopies. Although slowed 10-fold to ~6 ps, energy transfer from Chl a to B850 BChl a remained highly efficient. We measured faster energy-transfer time constants for Chl d (3.5 ps) and Chl f (2.7 ps), which have red-shifted absorption maxima compared to Chl a. BChl b, red-shifted from the native BChl a, gave extremely rapid (≤0.1 ps) transfer. These results show that modified LH2 complexes, combined with engineered (B)Chl biosynthesis pathways in vivo, have potential for retaining high efficiency whilst acquiring increased spectral range.

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Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22). Chemical Sciences, Geosciences & Biosciences Division

Grant/Contract Number:: SC0001035; FG02-94ER20137

OSTI ID:: 1482755

Alternate ID(s):: OSTI ID: 1566431

Journal Information:: Biochimica et Biophysica Acta - Bioenergetics, Journal Name: Biochimica et Biophysica Acta - Bioenergetics Journal Issue: 3 Vol. 1860; ISSN 0005-2728

Publisher:: ElsevierCopyright Statement

Country of Publication:: Netherlands

Language:: English

References (79)

Plasmon-Enhanced Photocurrent of Photosynthetic Pigment Proteins on Nanoporous Silver Friebe, Vincent M.; Delgado, Juan D.; Swainsbury, David J. K. Advanced Functional Materials, Vol. 26, Issue 2 https://doi.org/10.1002/adfm.201504020	journal	December 2015
Enhanced Output from Biohybrid Photoelectrochemical Transparent Tandem Cells Integrating Photosynthetic Proteins Genetically Modified for Expanded Solar Energy Harvesting Ravi, Sai Kishore; Yu, Zhimeng; Swainsbury, David J. K. Advanced Energy Materials, Vol. 7, Issue 7 https://doi.org/10.1002/aenm.201601821	journal	December 2016
UCSF Chimera?A visualization system for exploratory research and analysis Pettersen, Eric F.; Goddard, Thomas D.; Huang, Conrad C. Journal of Computational Chemistry, Vol. 25, Issue 13 https://doi.org/10.1002/jcc.20084	journal	January 2004
Cloning and heterologous expression of chlorophyll a synthase in Rhodobacter sphaeroides Ipekoğlu, Emre M.; Göçmen, Koray; Öz, Mehmet T. Journal of Basic Microbiology, Vol. 57, Issue 3 https://doi.org/10.1002/jobm.201600580	journal	November 2016
Projection structures of three photosynthetic complexes from Rhodobacter sphaeroides : LH2 at 6 Å, LH1 and RC-LH1 at 25 Å 1 1Edited by K. Nagai Walz, Thomas; Jamieson, Stuart J.; Bowers, Claire M. Journal of Molecular Biology, Vol. 282, Issue 4 https://doi.org/10.1006/jmbi.1998.2050	journal	October 1998
Versatile design of biohybrid light-harvesting architectures to tune location, density, and spectral coverage of attached synthetic chromophores for enhanced energy capture Harris, Michelle A.; Jiang, Jianbing; Niedzwiedzki, Dariusz M. Photosynthesis Research, Vol. 121, Issue 1 https://doi.org/10.1007/s11120-014-9993-8	journal	March 2014
The role of the quinone pool in the cyclic electron-transfer chain of Rhodopseudomonas sphaeroides A modified Q-cycle mechanism Crofts, A. R.; Meinhardt, S. W.; Jones, K. R. Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 723, Issue 2 https://doi.org/10.1016/0005-2728(83)90120-2	journal	May 1983
Pigment organization of the B800–850 antenna complex of Rhodopseudomonas sphaeroides Kramer, Herman J. M.; van Grondelle, Rienk; Hunter, C. Neil Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 765, Issue 2 https://doi.org/10.1016/0005-2728(84)90009-4	journal	May 1984
The effects of lithium dodecyl sulfate and sodium borohydride on the absorption spectrum of the B800–850 light-harvesting complex from Rhodopseudomonas acidophila 7750 Chadwick, Barry. W.; Zhang, Chaoying; Cogdell, Richard J. Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 893, Issue 3 https://doi.org/10.1016/0005-2728(87)90096-X	journal	October 1987
A resonance Raman investigation of the effect of lithium dodecyl sulfate on the B800–850 light-harvesting protein of Rhodopseudomonas acidophila 7750 Robert, Bruno; Frank, Harry A. Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 934, Issue 3 https://doi.org/10.1016/0005-2728(88)90098-9	journal	July 1988
Probing the B800 bacteriochlorophyll binding site of the accessory light-harvesting complex from Rhodobacter sphaeroides using site-directed mutants. I. Mutagenesis, effects on binding, function and electrochromic behaviour of its carotenoids Crielaard, Wim; Visschers, Ronald W.; Fowler, Gregory J. S. Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1183, Issue 3 https://doi.org/10.1016/0005-2728(94)90074-4	journal	January 1994
Probing the B800 bacteriochlorophyll binding site of the accessory light-harvesting complex from Rhodobacter sphaeroides using site-directed mutants. II. A low-temperature spectroscopy study of structural aspects of the pigment-protein conformation Visschers, Ronald W.; Crielaard, Wim; Fowler, Gregory J. S. Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1183, Issue 3 https://doi.org/10.1016/0005-2728(94)90075-2	journal	January 1994
Modified reaction centers from Rhodobacter sphaeroides R26: Exchange of monomeric bacteriochlorophyll with 13 ² -hydroxy-bacteriochlorophyll Struck, A.; Scheer, H. FEBS Letters, Vol. 261, Issue 2 https://doi.org/10.1016/0014-5793(90)80598-D	journal	February 1990
Modified reaction centers from Rhodobacter sphaeroides R26: 2: Bacteriochlorophylls with modified C-3 substituents at sites B _A and B _B Struck, A.; Cmiel, E.; Katheder, I. FEBS Letters, Vol. 268, Issue 1 https://doi.org/10.1016/0014-5793(90)81003-7	journal	July 1990
Efficiency of light energy conversion to hydrogen by the photosynthetic bacterium Rhodobacter sphaeroides Miyake, J.; Kawamura, S. International Journal of Hydrogen Energy, Vol. 12, Issue 3 https://doi.org/10.1016/0360-3199(87)90146-7	journal	January 1987
Small mobilizable multi-purpose cloning vectors derived from the Escherichia coli plasmids pK18 and pK19: selection of defined deletions in the chromosome of Corynebacterium glutamicum Schäfer, Andreas; Tauch, Andreas; Jäger, Wolfgang Gene, Vol. 145, Issue 1, p. 69-73 https://doi.org/10.1016/0378-1119(94)90324-7	journal	July 1994
Determination of accurate extinction coefficients and simultaneous equations for assaying chlorophylls a and b extracted with four different solvents: verification of the concentration of chlorophyll standards by atomic absorption spectroscopy Porra, R. J.; Thompson, W. A.; Kriedemann, P. E. Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 975, Issue 3 https://doi.org/10.1016/S0005-2728(89)80347-0	journal	August 1989
Reconstitution of the B800 bacteriochlorophylls in the peripheral light harvesting complex B800–850 of Rhodobacter sphaeroides 2.4.1 with BChl a and modified (bacterio-)chlorophylls Bandilla, Michael; Ücker, Beate; Ram, Marija Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1364, Issue 3 https://doi.org/10.1016/S0005-2728(98)00086-3	journal	May 1998
B800→B850 Energy Transfer Mechanism in Bacterial LH2 Complexes Investigated by B800 Pigment Exchange Herek, J. L.; Fraser, N. J.; Pullerits, T. Biophysical Journal, Vol. 78, Issue 5 https://doi.org/10.1016/S0006-3495(00)76803-2	journal	May 2000
Efficient Energy Transfer from the Carotenoid S2 State in a Photosynthetic Light-Harvesting Complex Macpherson, Alisdair N.; Arellano, Juan B.; Fraser, Niall J. Biophysical Journal, Vol. 80, Issue 2 https://doi.org/10.1016/S0006-3495(01)76071-7	journal	February 2001
Probing the binding sites of exchanged chlorophyll a in LH2 by Raman and site-selection fluorescence spectroscopies Gall, Andrew; Robert, Bruno; Cogdell, Richard J. FEBS Letters, Vol. 491, Issue 1-2 https://doi.org/10.1016/S0014-5793(01)02161-5	journal	February 2001
Bacteriochlorin-protein interactions in native B800-B850, B800 deficient and B800-Bchl a _p -reconstituted complexes from Rhodopseudomonas acidophila , strain 10050 Gall, Andrew; Fraser, Niall J.; Bellissent-Funel, Marie-Claire FEBS Letters, Vol. 449, Issue 2-3 https://doi.org/10.1016/S0014-5793(99)00410-X	journal	April 1999
The Structure and Thermal Motion of the B800–850 LH2 Complex from Rps.acidophila at 2.0Å Resolution and 100K: New Structural Features and Functionally Relevant Motions Papiz, Miroslav Z.; Prince, Steve M.; Howard, Tina Journal of Molecular Biology, Vol. 326, Issue 5 https://doi.org/10.1016/S0022-2836(03)00024-X	journal	March 2003
Extinction coefficient for red-shifted chlorophylls: Chlorophyll d and chlorophyll f Li, Yaqiong; Scales, Nicholas; Blankenship, Robert E. Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1817, Issue 8 https://doi.org/10.1016/j.bbabio.2012.02.026	journal	August 2012
Integration of energy and electron transfer processes in the photosynthetic membrane of Rhodobacter sphaeroides Cartron, Michaël L.; Olsen, John D.; Sener, Melih Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1837, Issue 10 https://doi.org/10.1016/j.bbabio.2014.02.003	journal	October 2014
Engineered biosynthesis of bacteriochlorophyll b in Rhodobacter sphaeroides Canniffe, Daniel P.; Hunter, C. Neil Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1837, Issue 10 https://doi.org/10.1016/j.bbabio.2014.07.011	journal	October 2014
Assembly of functional photosystem complexes in Rhodobacter sphaeroides incorporating carotenoids from the spirilloxanthin pathway Chi, Shuang C.; Mothersole, David J.; Dilbeck, Preston Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1847, Issue 2 https://doi.org/10.1016/j.bbabio.2014.10.004	journal	February 2015
Dimerization of core complexes as an efficient strategy for energy trapping in Rhodobacter sphaeroides Chenchiliyan, Manoop; Timpmann, Kõu; Jalviste, Erko Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1857, Issue 6 https://doi.org/10.1016/j.bbabio.2016.03.020	journal	June 2016
Engineering of a calcium-ion binding site into the RC-LH1-PufX complex of Rhodobacter sphaeroides to enable ion-dependent spectral red-shifting Swainsbury, David J. K.; Martin, Elizabeth C.; Vasilev, Cvetelin Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1858, Issue 11 https://doi.org/10.1016/j.bbabio.2017.08.009	journal	November 2017
Probing the local lipid environment of the cytochrome bc1 and Synechocystis sp. PCC 6803 cytochrome b6f complexes with styrene maleic acid Swainsbury, David J. K.; Proctor, Matthew S.; Hitchcock, Andrew Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1859, Issue 3 https://doi.org/10.1016/j.bbabio.2017.12.005	journal	March 2018
Engineered biosynthesis of bacteriochlorophyll gF in Rhodobacter sphaeroides Ortega-Ramos, Marcia; Canniffe, Daniel P.; Radle, Matthew I. Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1859, Issue 7 https://doi.org/10.1016/j.bbabio.2018.02.006	journal	July 2018
Insertion of chlorophyll a derivatives into the binding sites of B800 bacteriochlorophyll a in light-harvesting complex 2 from the purple photosynthetic bacterium Rhodoblastus acidophilus Saga, Yoshitaka; Amari, Kenta; Miyagi, Kanji Journal of Photochemistry and Photobiology A: Chemistry, Vol. 353 https://doi.org/10.1016/j.jphotochem.2017.07.039	journal	February 2018
The architecture and function of the light-harvesting apparatus of purple bacteria: from single molecules to in vivo membranes Cogdell, Richard J.; Gall, Andrew; Köhler, Jürgen Quarterly Reviews of Biophysics, Vol. 39, Issue 3 https://doi.org/10.1017/S0033583506004434	journal	August 2006
Top-Down Mass Spectrometry Analysis of Membrane-Bound Light-Harvesting Complex 2 from Rhodobacter sphaeroides Lu, Yue; Zhang, Hao; Cui, Weidong Biochemistry, Vol. 54, Issue 49 https://doi.org/10.1021/acs.biochem.5b00959	journal	November 2015
Reversible Changes in the Structural Features of Photosynthetic Light-Harvesting Complex 2 by Removal and Reconstitution of B800 Bacteriochlorophyll a Pigments Saga, Yoshitaka; Hirota, Keiya; Asakawa, Hitoshi Biochemistry, Vol. 56, Issue 27 https://doi.org/10.1021/acs.biochem.7b00267	journal	June 2017
Multireference Excitation Energies for Bacteriochlorophylls A within Light Harvesting System 2 Anda, André; Hansen, Thorsten; De Vico, Luca Journal of Chemical Theory and Computation, Vol. 12, Issue 3 https://doi.org/10.1021/acs.jctc.5b01104	journal	February 2016
Strong Coupling of Localized Surface Plasmons to Excitons in Light-Harvesting Complexes Tsargorodska, Anna; Cartron, Michaël L.; Vasilev, Cvetelin Nano Letters, Vol. 16, Issue 11 https://doi.org/10.1021/acs.nanolett.6b02661	journal	October 2016
Direct Imaging of Protein Organization in an Intact Bacterial Organelle Using High-Resolution Atomic Force Microscopy Kumar, Sandip; Cartron, Michaël L.; Mullin, Nic ACS Nano, Vol. 11, Issue 1 https://doi.org/10.1021/acsnano.6b05647	journal	November 2016
Biosynthesis of Chlorophyll a in a Purple Bacterial Phototroph and Assembly into a Plant Chlorophyll–Protein Complex Hitchcock, Andrew; Jackson, Philip J.; Chidgey, Jack W. ACS Synthetic Biology, Vol. 5, Issue 9 https://doi.org/10.1021/acssynbio.6b00069	journal	May 2016
Trapping Kinetics in Mutants of the Photosynthetic Purple Bacterium Rhodobacter sphaeroides: Influence of the Charge Separation Rate and Consequences for the Rate-Limiting Step in the Light-Harvesting Process Beekman, Lucas M. P.; van Mourik, Frank; Jones, Michael R. Biochemistry, Vol. 33, Issue 11 https://doi.org/10.1021/bi00177a001	journal	March 1994
Enhanced rates of subpicosecond energy transfer in blue-shifted light-harvesting LH2 mutants of Rhodobacter sphaeroides. Hess, S.; Visscher, K. J.; Pullerits, T. Biochemistry, Vol. 33, Issue 27 https://doi.org/10.1021/bi00193a017	journal	July 1994
Probing the bacteriochlorophyll binding site by reconstitution of the light-harvesting complex of Rhodospirillum rubrum with bacteriochlorophyll a analogs Parkes-Loach, Pamela S.; Michalski, Tomasz J.; Bass, Wendy J. Biochemistry, Vol. 29, Issue 12 https://doi.org/10.1021/bi00464a010	journal	March 1990
Excitation Energy Transfer between the B850 and B875 Antenna Complexes of Rhodobacter sphaeroides ^† Nagarajan, V.; Parson, W. W. Biochemistry, Vol. 36, Issue 8 https://doi.org/10.1021/bi962534b	journal	February 1997
The Role of βArg _- ₁₀ in the B800 Bacteriochlorophyll and Carotenoid Pigment Environment within the Light-Harvesting LH2 Complex of Rhodobacter sphaeroides ^† Fowler, Gregory J. S.; Hess, Susan; Pullerits, Tonu Biochemistry, Vol. 36, Issue 37 https://doi.org/10.1021/bi9626315	journal	September 1997
Influence of the Protein Binding Site on the Absorption Properties of the Monomeric Bacteriochlorophyll in Rhodobacter sphaeroides LH2 Complex ^† Gall, Andrew; Fowler, Gregory J. S.; Hunter, C. Neil Biochemistry, Vol. 36, Issue 51 https://doi.org/10.1021/bi9717237	journal	December 1997
Reconstitution of Core Light-Harvesting Complexes of Photosynthetic Bacteria Using Chemically Synthesized Polypeptides. 2. Determination of Structural Features That Stabilize Complex Formation and Their Implications for the Structure of the Subunit Complex ^† Kehoe, John W.; Meadows, Kelley A.; Parkes-Loach, Pamela S. Biochemistry, Vol. 37, Issue 10 https://doi.org/10.1021/bi9722709	journal	March 1998
Identification of the Upper Exciton Component of the B850 Bacteriochlorophylls of the LH2 Antenna Complex, Using a B800-Free Mutant of Rhodobacter sphaeroides ^† Koolhaas, M. H. C.; Frese, R. N.; Fowler, G. J. S. Biochemistry, Vol. 37, Issue 14 https://doi.org/10.1021/bi973036l	journal	April 1998
Selective Release, Removal, and Reconstitution of Bacteriochlorophyll a Molecules into the B800 Sites of LH2 Complexes from Rhodopseudomonas acidophila 10050 ^† Fraser, Niall J.; Dominy, Peter J.; Ücker, Beaté Biochemistry, Vol. 38, Issue 30 https://doi.org/10.1021/bi990796i	journal	July 1999
Structure of bacteriochlorophyll b Scheer, Hugo; Svec, Walter A.; Cope, Ben T. Journal of the American Chemical Society, Vol. 96, Issue 11 https://doi.org/10.1021/ja00818a092	journal	May 1974
Studies on the Chemical and Photochemical Oxidation of Bacteriochlorophyll ¹ Smith, John R. Lindsay; Calvin, Melvin Journal of the American Chemical Society, Vol. 88, Issue 19 https://doi.org/10.1021/ja00971a036	journal	October 1966
Biohybrid Photosynthetic Antenna Complexes for Enhanced Light-Harvesting Springer, Joseph W.; Parkes-Loach, Pamela S.; Reddy, Kanumuri Ramesh Journal of the American Chemical Society, Vol. 134, Issue 10 https://doi.org/10.1021/ja207390y	journal	February 2012
Reengineering the Optical Absorption Cross-Section of Photosynthetic Reaction Centers Dutta, Palash K.; Lin, Su; Loskutov, Andrey Journal of the American Chemical Society, Vol. 136, Issue 12 https://doi.org/10.1021/ja411843k	journal	March 2014
Extension of Light-Harvesting Ability of Photosynthetic Light-Harvesting Complex 2 (LH2) through Ultrafast Energy Transfer from Covalently Attached Artificial Chromophores Yoneda, Yusuke; Noji, Tomoyasu; Katayama, Tetsuro Journal of the American Chemical Society, Vol. 137, Issue 40 https://doi.org/10.1021/jacs.5b08508	journal	September 2015
Composition and optical properties of reaction centre core complexes from the green sulfur bacteria Prosthecochloris aestuarii and Chlorobium tepidum Permentier, Hjalmar P. Photosynthesis Research, Vol. 64, Issue 1, p. 27-39 https://doi.org/10.1023/A:1026515027824	journal	April 2000
Chlorophyll d as a major pigment Miyashita, Hideaki; Ikemoto, Hisato; Kurano, Norihide Nature, Vol. 383, Issue 6599 https://doi.org/10.1038/383402a0	journal	October 1996
Augmenting light coverage for photosynthesis through YFP-enhanced charge separation at the Rhodobacter sphaeroides reaction centre Grayson, Katie J.; Faries, Kaitlyn M.; Huang, Xia Nature Communications, Vol. 8, Issue 1 https://doi.org/10.1038/ncomms13972	journal	January 2017
Mapping the ultrafast flow of harvested solar energy in living photosynthetic cells Dahlberg, Peter D.; Ting, Po-Chieh; Massey, Sara C. Nature Communications, Vol. 8, Issue 1 https://doi.org/10.1038/s41467-017-01124-z	journal	October 2017
Cryo-EM structure of the Blastochloris viridis LH1–RC complex at 2.9 Å Qian, Pu; Siebert, C. Alistair; Wang, Peiyi Nature, Vol. 556, Issue 7700 https://doi.org/10.1038/s41586-018-0014-5	journal	April 2018
Theoretical study of excitation transfer from modified B800 rings of the LH II antenna complex of Rps. acidophila Linnanto, J.; Korppi-Tommola, J. E. I. Physical Chemistry Chemical Physics, Vol. 4, Issue 14 https://doi.org/10.1039/b108338b	journal	June 2002
Palette of lipophilic bioconjugatable bacteriochlorins for construction of biohybrid light-harvesting architectures Reddy, Kanumuri Ramesh; Jiang, Jianbing; Krayer, Michael Chemical Science, Vol. 4, Issue 5 https://doi.org/10.1039/c3sc22317e	journal	January 2013
Integration of multiple chromophores with native photosynthetic antennas to enhance solar energy capture and delivery Harris, Michelle A.; Parkes-Loach, Pamela S.; Springer, Joseph W. Chemical Science, Vol. 4, Issue 10 https://doi.org/10.1039/c3sc51518d	journal	January 2013
Identification of an 8-vinyl reductase involved in bacteriochlorophyll biosynthesis in Rhodobacter sphaeroides and evidence for the existence of a third distinct class of the enzyme Canniffe, Daniel P.; Jackson, Philip J.; Hollingshead, Sarah Biochemical Journal, Vol. 450, Issue 2 https://doi.org/10.1042/BJ20121723	journal	February 2013
The petite purple photosynthetic powerpack Jones, Michael R. Biochemical Society Transactions, Vol. 37, Issue 2 https://doi.org/10.1042/BST0370400	journal	March 2009
Probing structure–function relationships in early events in photosynthesis using a chimeric photocomplex Nagashima, Kenji V. P.; Sasaki, Mai; Hashimoto, Kanako Proceedings of the National Academy of Sciences, Vol. 114, Issue 41 https://doi.org/10.1073/pnas.1703584114	journal	September 2017
Isolation and characterization of the pigment-protein complexes of Rhodopseudomonas sphaeroides by lithium dodecyl sulfate/polyacrylamide gel electrophoresis. Broglie, R. M.; Hunter, C. N.; Delepelaire, P. Proceedings of the National Academy of Sciences, Vol. 77, Issue 1 https://doi.org/10.1073/pnas.77.1.87	journal	January 1980
B850 pigment-protein complex of Rhodopseudomonas sphaeroides: Extinction coefficients, circular dichroism, and the reversible binding of bacteriochlorophyll Clayton, R. K.; Clayton, B. J. Proceedings of the National Academy of Sciences, Vol. 78, Issue 9 https://doi.org/10.1073/pnas.78.9.5583	journal	September 1981
Temporally and spectrally resolved subpicosecond energy transfer within the peripheral antenna complex (LH2) and from LH2 to the core antenna complex in photosynthetic purple bacteria. Hess, S.; Chachisvilis, M.; Timpmann, K. Proceedings of the National Academy of Sciences, Vol. 92, Issue 26 https://doi.org/10.1073/pnas.92.26.12333	journal	December 1995
Interference lithographic nanopatterning of plant and bacterial light-harvesting complexes on gold substrates Patole, Samson; Vasilev, Cvetelin; El-Zubir, Osama Interface Focus, Vol. 5, Issue 4 https://doi.org/10.1098/rsfs.2015.0005	journal	August 2015
Generic Assignments, Strain Histories and Properties of Pure Cultures of Cyanobacteria Stanier, Roger Y.; Deruelles, Josette; Rippka, Rosmarie Microbiology, Vol. 111, Issue 1, p. 1-61 https://doi.org/10.1099/00221287-111-1-1	journal	March 1979
Membrane invagination in Rhodobacter sphaeroides is initiated at curved regions of the cytoplasmic membrane, then forms both budded and fully detached spherical vesicles: Membrane biogenesis in Rba. sphaeroides Tucker, Jaimey D.; Siebert, C. Alistair; Escalante, Maryana Molecular Microbiology, Vol. 76, Issue 4 https://doi.org/10.1111/j.1365-2958.2010.07153.x	journal	April 2010
Triplet State Energy Transfer Between the Primary Donor and the Carotenoid in Rhodobacter sphaeroides R-26.1 Reaction Centers Exchanged with Modified Bacteriochlorophyll Pigments and Reconstituted with Spheroidene Frank, Harry A.; Chynwat, Veeradej; Posteraro, Anthony Photochemistry and Photobiology, Vol. 64, Issue 5 https://doi.org/10.1111/j.1751-1097.1996.tb01842.x	journal	November 1996
PucC and LhaA direct efficient assembly of the light-harvesting complexes in Rhodobacter sphaeroides : Photosystem assembly in Mothersole, David J.; Jackson, Philip J.; Vasilev, Cvetelin Molecular Microbiology, Vol. 99, Issue 2 https://doi.org/10.1111/mmi.13235	journal	November 2015
A Red-Shifted Chlorophyll: Fig. 1 Chen, Min; Schliep, Martin; Willows, Robert D. Science, Vol. 329, Issue 5997 https://doi.org/10.1126/science.1191127	journal	August 2010
Heterologous Production of the Photosynthetic Reaction Center and Light Harvesting 1 Complexes of the Thermophile Thermochromatium tepidum in the Mesophile Rhodobacter sphaeroides and Thermal Stability of a Hybrid Core Complex Jun, D.; Huang, V.; Beatty, J. T. Applied and Environmental Microbiology, Vol. 83, Issue 20 https://doi.org/10.1128/AEM.01481-17	journal	August 2017
Two Unrelated 8-Vinyl Reductases Ensure Production of Mature Chlorophylls in Acaryochloris marina Chen, Guangyu E.; Hitchcock, Andrew; Jackson, Philip J. Journal of Bacteriology, Vol. 198, Issue 9 https://doi.org/10.1128/JB.00925-15	journal	February 2016
A Second and Unusual pucBA Operon of Rhodobacter sphaeroides 2.4.1: Genetics and Function of the Encoded Polypeptides Zeng, X.; Choudhary, M.; Kaplan, S. Journal of Bacteriology, Vol. 185, Issue 20 https://doi.org/10.1128/JB.185.20.6171-6184.2003	journal	October 2003
Chlorophyll Biosynthesis in Bacteria: The Origins of Structural and Functional Diversity Chew, Aline Gomez Maqueo; Bryant, Donald A. Annual Review of Microbiology, Vol. 61, Issue 1 https://doi.org/10.1146/annurev.micro.61.080706.093242	journal	October 2007
Reconstitution of Chlorophyll d into the Bacterial Photosynthetic Light-harvesting Protein LH2 Saga, Yoshitaka; Yamashita, Madoka; Imanishi, Michie Chemistry Letters, Vol. 47, Issue 8 https://doi.org/10.1246/cl.180483	journal	August 2018
Overall energy conversion efficiency of a photosynthetic vesicle Sener, Melih; Strumpfer, Johan; Singharoy, Abhishek eLife, Vol. 5 https://doi.org/10.7554/eLife.09541	journal	August 2016

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