Structural basis for the second step of group II intron splicing

Chan, Russell T.; Peters, Jessica K.; Robart, Aaron R.; Wiryaman, Timothy; Rajashankar, Kanagalaghatta R.; Toor, Navtej

doi:10.1038/s41467-018-06678-0

Structural basis for the second step of group II intron splicing

Journal Article · Thu Nov 08 00:00:00 EST 2018 · Nature Communications

DOI:https://doi.org/10.1038/s41467-018-06678-0· OSTI ID:1482239

Chan, Russell T. ^[1]; Peters, Jessica K. ^[2]; Robart, Aaron R. ^[3]; Wiryaman, Timothy ^[2]; Rajashankar, Kanagalaghatta R. ^[4]; ^[2]

Univ. of California, San Diego, La Jolla, CA (United States); DOE/OSTI
Univ. of California, San Diego, La Jolla, CA (United States)
Univ. of California, San Diego, La Jolla, CA (United States); West Virginia Univ., Morgantown, WV (United States)
Argonne National Lab. (ANL), Argonne, IL (United States)

The group II intron and the spliceosome share a common active site architecture and are thought to be evolutionarily related. Here we report the 3.7 Å crystal structure of a eukaryotic group II intron in the lariat-3' exon form, immediately preceding the second step of splicing, analogous to the spliceosomal P complex. This structure reveals the location of the intact 3' splice site within the catalytic core of the group II intron. The 3'-OH of the 5' exon is positioned in close proximity to the 3' splice site for nucleophilic attack and exon ligation. The active site undergoes conformational rearrangements with the catalytic triplex having different configurations before and after the second step of splicing. We describe a complete model for the second step of group II intron splicing that incorporates a dynamic catalytic triplex being responsible for creating the binding pocket for 3' splice site capture.

View Accepted Manuscript (DOE)

Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States). Advanced Photon Source (APS)

Sponsoring Organization:: NIH; USDOE

Grant/Contract Number:: AC02-06CH11357

OSTI ID:: 1482239

Journal Information:: Nature Communications, Journal Name: Nature Communications Journal Issue: 1 Vol. 9; ISSN 2041-1723

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: ENGLISH

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Branch site bulge conformations in domain 6 determine functional sugar puckers in group II intron splicing Plangger, Raphael; Juen, Michael Andreas; Hoernes, Thomas Philipp Nucleic Acids Research, Vol. 47, Issue 21 https://doi.org/10.1093/nar/gkz965	journal	October 2019
A Bioinformatic Method For Identifying Group II Introns In Organella Genomes Titov, Igor; Kobalo, Nikolay; Vorobyev, Denis Frontiers in Genetics, Vol. 10 https://doi.org/10.3389/fgene.2019.01135	journal	November 2019

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