Microbial community response and SDS-PAGE reveal possible mechanism of waste activated sludge acidification enhanced by microaeration coupled thermophilic pretreatment

Hou, Ya-Nan; Yang, Chunxue; Zhou, Aijuan; Liu, Wenzong; Liu, Chong; Cai, Wei-Wei; Zhou, Jizhong; Wang, Ai-Jie

doi:10.1016/j.procbio.2017.09.010

Microbial community response and SDS-PAGE reveal possible mechanism of waste activated sludge acidification enhanced by microaeration coupled thermophilic pretreatment

Journal Article · Wed Sep 13 00:00:00 EDT 2017 · Process Biochemistry

DOI:https://doi.org/10.1016/j.procbio.2017.09.010· OSTI ID:1479367

Hou, Ya-Nan ^[1]; Yang, Chunxue ^[1]; Zhou, Aijuan ^[2]; Liu, Wenzong ^[3]; Liu, Chong ^[1]; Cai, Wei-Wei ^[1]; Zhou, Jizhong ^[4]; Wang, Ai-Jie ^[5]

Harbin Inst. of Technology (China). State Key Lab. of Urban Water Resource and Environment
Taiyuan Univ. of Technology (China). College of Environmental Science and Engineering
Chinese Academy of Sciences (CAS), Beijing (China). Key Lab. of Environmental Biotechnology. Research Center for Eco-Environmental Sciences
Harbin Inst. of Technology (China). State Key Lab. of Urban Water Resource and Environment; Univ. of Oklahoma, Norman, OK (United States). Inst. for Environmental Genomics. Dept. of Microbiology and Plant Biology
Harbin Inst. of Technology (China). State Key Lab. of Urban Water Resource and Environment; Chinese Academy of Sciences (CAS), Beijing (China). Key Lab. of Environmental Biotechnology. Research Center for Eco-Environmental Sciences

Aiming to strengthen the performance of waste activated sludge digestion, pretreatment is a prerequisite for keeping operations within an industrially acceptable time- frame. The performance of microaeration coupled with thermophilic (MT) pretreatment on waste activated sludge solubilization and acidification was investigated. The results showed that the maximum soluble organics concentrations, including soluble proteins and carbohydrates, reached 2290 mg COD/L in 24 h when the ventilation rate and temperature were 0.05 vvm and 70 °C. SDS-polyacrylamide gel electrophoresis (SDS-PAGE) test on pure-culture (E.coli) and scanning electron microscopy analysis indicated that MT pretreatment effectively destroyed microbial cell wall and resulted in an increase in soluble proteins. Polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) analysis revealed that MT pretreatment reduced the diversity of the bacterial community during pretreatment. Along with the dissolution of a large quantity of organic matter, microbial species such as Lactococcus and Methyloversatilis bloomed at the end of acidification period, which contributed to SCFAs production. Finally, this study revealed that MT pretreatment facilitated the WAS hydrolysis efficiency and enhanced SCFAs (3149 mg COD/L with fermentation for 96 h) production, especially for acetic acid (50%) accumulation, which provides a new perspective for the application and recovery of sludge resources.

View Accepted Manuscript (DOE)

Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: China Postdoctoral Science Foundation; Chinese Academy of Sciences (CAS); National Natural Science Foundation of China (NSFC); Scientific and Technological Project of Shanxi Province (China); USDOE

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 1479367

Journal Information:: Process Biochemistry, Journal Name: Process Biochemistry Vol. 64; ISSN 1359-5113

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

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59 BASIC BIOLOGICAL SCIENCES
micro-aeration
pretreatment
short-chain fatty acids (SCFAs)
thermophilic
waste activated sludge (WAS)

Microbial community response and SDS-PAGE reveal possible mechanism of waste activated sludge acidification enhanced by microaeration coupled thermophilic pretreatment

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