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Title: Improved production of the NiFe-hydrogenase from Pyrococcus furiosus by increased expression of maturation genes

Journal Article · · Protein Engineering, Design and Selection
 [1];  [1];  [1];  [1]
  1. Department of Biochemistry and Molecular Biology, University of Georgia, Athens, GA, USA

Sponsoring Organization:
USDOE Office of Energy Efficiency and Renewable Energy (EERE)
OSTI ID:
1479121
Journal Information:
Protein Engineering, Design and Selection, Journal Name: Protein Engineering, Design and Selection Vol. 31 Journal Issue: 9; ISSN 1741-0126
Publisher:
Oxford University PressCopyright Statement
Country of Publication:
United Kingdom
Language:
English
Citation Metrics:
Cited by: 5 works
Citation information provided by
Web of Science

References (43)

Dual organism design cycle reveals small subunit substitutions that improve [NiFe] hydrogenase hydrogen evolution journal January 2013
Cleaning the Air and Improving Health with Hydrogen Fuel-Cell Vehicles journal June 2005
Purification and characterization of Desulfovibrio vulgaris (Hildenborough) hydrogenase expressed in Escherichia coli journal January 1987
Occurrence, Classification, and Biological Function of Hydrogenases:  An Overview journal October 2007
High yield purification of a tagged cytoplasmic [NiFe]-hydrogenase and a catalytically-active nickel-free intermediate form journal March 2015
Metal insertion into NiFe-hydrogenases journal August 2002
Water Splitting for High-Yield Hydrogen Production Energized by Biomass Xylooligosaccharides Catalyzed by an Enzyme Cocktail journal August 2016
Reinvestigation of the Steady-State Kinetics and Physiological Function of the Soluble NiFe-Hydrogenase I of Pyrococcus furiosus journal December 2007
Engineering Hydrogen Gas Production from Formate in a Hyperthermophile by Heterologous Production of an 18-Subunit Membrane-bound Complex journal December 2013
Intact Functional Fourteen-subunit Respiratory Membrane-bound [NiFe]-Hydrogenase Complex of the Hyperthermophilic Archaeon Pyrococcus furiosus journal May 2014
[NiFe]-Hydrogenase Maturation journal March 2016
Spontaneous High-Yield Production of Hydrogen from Cellulosic Materials and Water Catalyzed by Enzyme Cocktails journal February 2009
Maturation of Hydrogenases book September 2006
Oxygen-Tolerant [NiFe]-Hydrogenases: The Individual and Collective Importance of Supernumerary Cysteines at the Proximal Fe-S Cluster journal October 2011
High-yield hydrogen production from biomass by in vitro metabolic engineering: Mixed sugars coutilization and kinetic modeling journal April 2015
Re-engineering a NiFe hydrogenase to increase the H 2 production bias while maintaining native levels of O 2 tolerance journal January 2016
Conversion of the central [4Fe-4S] cluster into a [3Fe-4S] cluster leads to reduced hydrogen-uptake activity of the F420-reducing hydrogenase of Methanococcus voltae: Intramolecular electron transport in hydrogenases journal November 2000
In vitro metabolic engineering of hydrogen production at theoretical yield from sucrose journal July 2014
An Innovative Cloning Platform Enables Large-Scale Production and Maturation of an Oxygen-Tolerant [NiFe]-Hydrogenase from Cupriavidus necator in Escherichia coli journal July 2013
NADPH-generating systems in bacteria and archaea journal July 2015
A unique iron-sulfur cluster is crucial for oxygen tolerance of a [NiFe]-hydrogenase journal March 2011
Biological hydrogen production: prospects and challenges journal May 2010
Development of a synthetic pathway to convert glucose to hydrogen using cell free extracts journal August 2015
Deletion of acetyl-CoA synthetases I and II increases production of 3-hydroxypropionate by the metabolically-engineered hyperthermophile Pyrococcus furiosus journal March 2014
Changing the Ligation of the Distal [4Fe4S] Cluster in NiFe Hydrogenase Impairs Inter- and Intramolecular Electron Transfers journal April 2006
Recombinant and in vitro expression systems for hydrogenases: new frontiers in basic and applied studies for biological and synthetic H2 production journal January 2009
Characterization of Hydrogenase II from the Hyperthermophilic Archaeon Pyrococcus furiosus and Assessment of Its Role in Sulfur Reduction journal April 2000
Natural Competence in the Hyperthermophilic Archaeon Pyrococcus furiosus Facilitates Genetic Manipulation: Construction of Markerless Deletions of Genes Encoding the Two Cytoplasmic Hydrogenases journal February 2011
The crystal structure of an oxygen-tolerant hydrogenase uncovers a novel iron-sulphur centre journal October 2011
Glutamate Gated Proton-Coupled Electron Transfer Activity of a [NiFe]-Hydrogenase journal September 2016
Enzymatic production of biohydrogen: Biotechnology journal June 2000
DNA Microarray Analysis of the Hyperthermophilic Archaeon Pyrococcus furiosus: Evidence for a New Type of Sulfur-Reducing Enzyme Complex journal December 2001
[3Fe-4S] to [4Fe-4S] cluster conversion in Desulfovibrio fructosovorans [NiFe] hydrogenase by site-directed mutagenesis journal September 1998
Genes encoding the NAD-reducing hydrogenase of Rhodococcus opacus MR11 journal April 1997
Engineering Hyperthermophilic Archaeon Pyrococcus furiosus to Overproduce Its Cytoplasmic [NiFe]-Hydrogenase journal December 2011
Maturation of [NiFe]-hydrogenases in Escherichia coli journal January 2007
Genetic analyses of the functions of [NiFe]-hydrogenase maturation endopeptidases in the hyperthermophilic archaeon Thermococcus kodakarensis journal October 2016
High-Yield Hydrogen Production from Starch and Water by a Synthetic Enzymatic Pathway journal May 2007
Engineering the respiratory membrane-bound hydrogenase of the hyperthermophilic archaeon Pyrococcus furiosus and characterization of the catalytically active cytoplasmic subcomplex journal December 2014
Heterologous Expression and Maturation of an NADP-Dependent [NiFe]-Hydrogenase: A Key Enzyme in Biofuel Production journal May 2010
Overlap extension PCR cloning: a simple and reliable way to create recombinant plasmids journal June 2010
High-Yield Production of Dihydrogen from Xylose by Using a Synthetic Enzyme Cascade in a Cell-Free System journal March 2013
Production and Application of a Soluble Hydrogenase from Pyrococcus furiosus journal January 2015

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Improved production of the NiFe-hydrogenase from Pyrococcus furiosus by increased expression of maturation genes
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