Structures of the fungal dynamin-related protein Vps1 reveal a unique, open helical architecture

Varlakhanova, Natalia V.; Alvarez, Frances J. D.; Brady, Tyler M.; Tornabene, Bryan A.; Hosford, Christopher J.; Chappie, Joshua S.; Zhang, Peijun; Ford, Marijn G. J.

doi:10.1083/jcb.201712021

Structures of the fungal dynamin-related protein Vps1 reveal a unique, open helical architecture

Journal Article · Tue Aug 07 04:00:00 EDT 2018 · Journal of Cell Biology

DOI:https://doi.org/10.1083/jcb.201712021· OSTI ID:1479024

^[1]; ^[2]; Brady, Tyler M. ^[1]; Tornabene, Bryan A. ^[1]; ^[3]; ^[3]; ^[4]; ^[1]

Department of Cell Biology, University of Pittsburgh School of Medicine, Pittsburgh, PA
Department of Structural Biology, University of Pittsburgh School of Medicine, Pittsburgh, PA
Department of Molecular Medicine, Cornell University, Ithaca, NY
Department of Structural Biology, University of Pittsburgh School of Medicine, Pittsburgh, PA; Division of Structural Biology, Wellcome Trust Centre for Human Genetics, University of Oxford, Oxford, UK; Electron Bio-Imaging Centre, Diamond Light Source, Harwell Science and Innovation Campus, Didcot, UK

Dynamin-related proteins (DRPs) are large multidomain GTPases required for diverse membrane-remodeling events. DRPs self-assemble into helical structures, but how these structures are tailored to their cellular targets remains unclear. We demonstrate that the fungal DRP Vps1 primarily localizes to and functions at the endosomal compartment. We present crystal structures of a Vps1 GTPase–bundle signaling element (BSE) fusion in different nucleotide states to capture GTP hydrolysis intermediates and concomitant conformational changes. Using cryoEM, we determined the structure of full-length GMPPCP-bound Vps1. The Vps1 helix is more open and flexible than that of dynamin. This is due to further opening of the BSEs away from the GTPase domains. A novel interface between adjacent GTPase domains forms in Vps1 instead of the contacts between the BSE and adjacent stalks and GTPase domains as seen in dynamin. Disruption of this interface abolishes Vps1 function in vivo. Hence, Vps1 exhibits a unique helical architecture, highlighting structural flexibilities of DRP self-assembly.

Research Organization:: Advanced Photon Source (APS), Argonne National Laboratory (ANL), Argonne, IL (US)

Sponsoring Organization:: NIH

OSTI ID:: 1479024

Journal Information:: Journal of Cell Biology, Journal Name: Journal of Cell Biology Journal Issue: 10 Vol. 217; ISSN 0021-9525

Publisher:: Rockefeller University Press

Country of Publication:: United States

Language:: ENGLISH

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