Designing seryl‐ tRNA synthetase for improved serylation of selenocysteine tRNA s

Fu, Xian; Crnković, Ana; Sevostyanova, Anastasia; Söll, Dieter

doi:10.1002/1873-3468.13271

Designing seryl‐ tRNA synthetase for improved serylation of selenocysteine tRNA s

Journal Article · Wed Oct 24 00:00:00 EDT 2018 · FEBS Letters

DOI:https://doi.org/10.1002/1873-3468.13271· OSTI ID:1478680

Fu, Xian ^[1]; ^[1]; ^[1]; ^[2]

Department of Molecular Biophysics &, Biochemistry Yale University New Haven CT USA
Department of Molecular Biophysics &, Biochemistry Yale University New Haven CT USA, Department of Chemistry Yale University New Haven CT USA

Selenocysteine (Sec) lacks a cognate aminoacyl‐ tRNA synthetase. Instead, seryl‐ tRNA synthetase (Ser RS ) produces Ser‐ tRNA ^S ^ec , which is subsequently converted by selenocysteine synthase to Sec‐ tRNA ^S ^ec . Escherichia coli Ser RS serylates tRNA ^S ^ec poorly; this may hinder efficient production of designer selenoproteins in vivo . Guided by structural modelling and selection for chloramphenicol acetyltransferase activity, we evolved three Ser RS variants capable of improved Ser‐ tRNA ^S ^ec synthesis. They display 10‐, 8‐, and 4‐fold increased k _cat / K _M values compared to wild‐type Ser RS using synthetic tRNA ^S ^ec species as substrates. The enzyme variants also facilitate in vivo read‐through of a UAG codon in the position of the critical serine ₁₄₆ of chloramphenicol acetyltransferase. These results indicate that the naturally evolved Ser RS is capable of further evolution for increased recognition of a specific tRNA isoacceptor.

View Accepted Manuscript (Publisher)

Sponsoring Organization:: USDOE

Grant/Contract Number:: NONE; FG02-98ER20311

OSTI ID:: 1478680

Alternate ID(s):: OSTI ID: 1610393

Journal Information:: FEBS Letters, Journal Name: FEBS Letters Journal Issue: 22 Vol. 592; ISSN 0014-5793

Publisher:: Wiley Blackwell (John Wiley & Sons)Copyright Statement

Country of Publication:: Netherlands

Language:: English

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