A guanine-flipping and sequestration mechanism for G-quadruplex unwinding by RecQ helicases

Voter, Andrew F.; Qiu, Yupeng; Tippana, Ramreddy; Myong, Sua; Keck, James L.

doi:10.1038/s41467-018-06751-8

A guanine-flipping and sequestration mechanism for G-quadruplex unwinding by RecQ helicases

Journal Article · Wed Oct 10 00:00:00 EDT 2018 · Nature Communications

DOI:https://doi.org/10.1038/s41467-018-06751-8· OSTI ID:1478097

^[1]; Qiu, Yupeng ^[2]; Tippana, Ramreddy ^[2]; Myong, Sua ^[2]; ^[3]

Univ. of Wisconsin School of Medicine and Public Health, Madison, WI (United States); DOE/OSTI
Johns Hopkins Univ., Baltimore, MD (United States)
Univ. of Wisconsin School of Medicine and Public Health, Madison, WI (United States)

Homeostatic regulation of G-quadruplexes (G4s), four-stranded structures that can form in guanine-rich nucleic acids, requires G4 unwinding helicases. The mechanisms that mediate G4 unwinding remain unknown. We report the structure of a bacterial RecQ DNA helicase bound to resolved G4 DNA. Unexpectedly, a guanine base from the unwound G4 is sequestered within a guanine-specific binding pocket. Disruption of the pocket in RecQ blocks G4 unwinding, but not G4 binding or duplex DNA unwinding, indicating its essential role in structure-specific G4 resolution. A novel guanine-flipping and sequestration model that may be applicable to other G4-resolving helicases emerges from these studies.

View Accepted Manuscript (DOE)

Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States). Advanced Photon Source (APS)

Sponsoring Organization:: NIH; USDOE Office of Science (SC), Basic Energy Sciences (BES). Scientific User Facilities Division

Grant/Contract Number:: AC02-06CH11357

OSTI ID:: 1478097

Journal Information:: Nature Communications, Journal Name: Nature Communications Journal Issue: 1 Vol. 9; ISSN 2041-1723

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: ENGLISH

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RNA G-Quadruplex is Resolved by Repetitive and ATP Dependent Mechanism of DHX36 Tippana, Ramreddy; Chen, Michael C.; Demeshkina, Natalia A. Biophysical Journal, Vol. 116, Issue 3 https://doi.org/10.1016/j.bpj.2018.11.2713	journal	February 2019
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