The chemical fingerprint of hair melanosomes by infrared nano-spectroscopy

Stanic, Vesna; Maia, Francisco Carlos Barbosa; Freitas, Raul de Oliveira; Montoro, Fabiano Emmanuel; Evans-Lutterodt, Kenneth

doi:10.1039/C8NR03146K

The chemical fingerprint of hair melanosomes by infrared nano-spectroscopy

Journal Article · Mon Jul 16 00:00:00 EDT 2018 · Nanoscale

DOI:https://doi.org/10.1039/C8NR03146K· OSTI ID:1477957

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Brazilian Synchrotron Light Lab., Campinas (Brazil)
Brazilian National Nanotechnology Lab., Campinas (Brazil)
Brookhaven National Lab. (BNL), Upton, NY (United States)

In situ characterization of the chemical and structural properties of black and white sheep hair was performed with a spatial resolution of 25 nm using infrared nano-spectroscopy. Comparing data sets from two types of hair allowed us to isolate the keratin FTIR fingerprint and so mark off chemical properties of the hair's melanosomes. From a polarization sensitive analysis of the nano-FTIR spectra, we showed that keratin intermediate filaments (IFs) present anisotropic molecular ordering. In stark contrast with white hair which does not contain melanosomes, in black hair, we spatially resolved single melanosomes and achieved unprecedented assignment of the vibrational modes of pheomelanin and eumelanin. The in situ experiment presented here avoids harsh chemical extractive methods used in previous studies. Lastly, our findings offer a basis for a better understanding of the keratin chemical and structural packing in different hair phenotypes as well as the involvement of melanosomes in hair color and biological functionality.

View Accepted Manuscript (DOE)

Research Organization:: Brookhaven National Laboratory (BNL), Upton, NY (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)

Grant/Contract Number:: SC0012704

OSTI ID:: 1477957

Report Number(s):: BNL--209180-2018-JAAM

Journal Information:: Nanoscale, Journal Name: Nanoscale Journal Issue: 29 Vol. 10; ISSN NANOHL; ISSN 2040-3364

Publisher:: Royal Society of ChemistryCopyright Statement

Country of Publication:: United States

Language:: English

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