Structure-specific DNA replication-fork recognition directs helicase and replication restart activities of the PriA helicase

Windgassen, Tricia A.; Leroux, Maxime; Satyshur, Kenneth A.; Sandler, Steven J.; Keck, James L.

doi:10.1073/pnas.1809842115

Structure-specific DNA replication-fork recognition directs helicase and replication restart activities of the PriA helicase

Journal Article · Mon Sep 10 00:00:00 EDT 2018 · Proceedings of the National Academy of Sciences of the United States of America

DOI:https://doi.org/10.1073/pnas.1809842115· OSTI ID:1477117

Windgassen, Tricia A. ^[1]; Leroux, Maxime ^[2]; Satyshur, Kenneth A. ^[1]; Sandler, Steven J. ^[2]; ^[1]

Univ. of Wisconsin School of Medicine and Public Health, Madison, WI (United States)
Univ. of Massachusetts, Amherst, MA (United States)

DNA replication restart, the essential process that reinitiates prematurely terminated genome replication reactions, relies on exquisitely specific recognition of abandoned DNA replication-fork structures. The PriA DNA helicase mediates this process in bacteria through mechanisms that remain poorly defined. We report the crystal structure of a PriA/replication-fork complex, which resolves leading-strand duplex DNA bound to the protein. Interaction with PriA unpairs one end of the DNA and sequesters the 3'-most nucleotide from the nascent leading strand into a conserved protein pocket. Cross-linking studies reveal a surface on the winged-helix domain of PriA that binds to parental duplex DNA. Deleting the winged-helix domain alters PriA’s structure-specific DNA unwinding properties and impairs its activity in vivo. Here, our observations lead to a model in which coordinated parental-, leading-, and lagging-strand DNA binding provide PriA with the structural specificity needed to act on abandoned DNA replication forks.

View Accepted Manuscript (DOE)

Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States)

Sponsoring Organization:: National Inst. of Health; National Science Foundation

OSTI ID:: 1477117

Journal Information:: Proceedings of the National Academy of Sciences of the United States of America, Journal Name: Proceedings of the National Academy of Sciences of the United States of America Journal Issue: 39 Vol. 115; ISSN 0027-8424

Publisher:: National Academy of SciencesCopyright Statement

Country of Publication:: United States

Language:: ENGLISH

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59 BASIC BIOLOGICAL SCIENCES
DNA repair
DNA replication restart
X-ray crystallography
cross-link mapping
protein–DNA complex

Structure-specific DNA replication-fork recognition directs helicase and replication restart activities of the PriA helicase

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