Enzymatic Adaptation of Podospora anserina to Different Plant Biomass Provides Leads to Optimized Commercial Enzyme Cocktails

Benocci, Tiziano; Daly, Paul; Aguilar‐Pontes, Maria Victoria; Lail, Kathleen; Wang, Mei; Lipzen, Anna; Ng, Vivian; Grigoriev, Igor V.; de Vries, Ronald P.

doi:10.1002/biot.201800185

Title: Enzymatic Adaptation of Podospora anserina to Different Plant Biomass Provides Leads to Optimized Commercial Enzyme Cocktails

Journal Article · Mon Oct 08 00:00:00 EDT 2018 · Biotechnology Journal

DOI:https://doi.org/10.1002/biot.201800185· OSTI ID:1476667

Benocci, Tiziano ^[1]; Daly, Paul ^[1]; Aguilar‐Pontes, Maria Victoria ^[1]; Lail, Kathleen ^[2]; Wang, Mei ^[2]; Lipzen, Anna ^[2]; Ng, Vivian ^[2]; Grigoriev, Igor V. ^[3]; de Vries, Ronald P. ^[1]

Fungal Physiology Westerdijk Fungal Biodiversity Institute &, Fungal Molecular Physiology Utrecht University Uppsalalaan 8 3584 CT Utrecht The Netherlands
US Department of Energy Joint Genome Institute 2800 Mitchell Drive Walnut Creek CA 94598 USA
US Department of Energy Joint Genome Institute 2800 Mitchell Drive Walnut Creek CA 94598 USA, Department of Plant and Microbial Biology University of California Berkeley Berkeley CA 94598 USA

As a late colonizer of herbivore dung, Podospora anserina has evolved an enzymatic machinery to degrade the more recalcitrant fraction of plant biomass, suggesting a great potential for biotechnology applications. The authors investigated its transcriptome during growth on two industrial feedstocks, soybean hulls (SBH) and corn stover (CS). Initially, CS and SBH results in the expression of hemicellulolytic and amylolytic genes, respectively, while at later time points a more diverse gene set is induced, especially for SBH. Substrate adaptation is also observed for carbon catabolism. Overall, SBH resulted in a larger diversity of expressed genes, confirming previous proteomics studies. The results not only provide an in depth view on the transcriptomic adaptation of P. anserina to substrate composition, but also point out strategies to improve saccharification of plant biomass at the industrial level.

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Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States); USDOE Joint Genome Institute (JGI), Walnut Creek, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC); NWO

Grant/Contract Number:: DE‐AC02‐05CH11231; AC02-05CH11231

OSTI ID:: 1476667

Alternate ID(s):: OSTI ID: 1476668; OSTI ID: 1559199

Journal Information:: Biotechnology Journal, Journal Name: Biotechnology Journal Vol. 14 Journal Issue: 4; ISSN 1860-6768

Publisher:: Wiley Blackwell (John Wiley & Sons)Copyright Statement

Country of Publication:: Germany

Language:: English

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Cited by: 10 works

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