Characterization of natural organic matter in low-carbon sediments: Extraction and analytical approaches

Fox, Patricia M.; Nico, Peter S.; Tfaily, Malak M.; Heckman, Katherine; Davis, James A.

doi:10.1016/j.orggeochem.2017.08.009

Characterization of natural organic matter in low-carbon sediments: Extraction and analytical approaches

Journal Article · Sat Aug 26 04:00:00 EDT 2017 · Organic Geochemistry

DOI:https://doi.org/10.1016/j.orggeochem.2017.08.009· OSTI ID:1476582

Fox, Patricia M. ^[1]; Nico, Peter S. ^[1]; Tfaily, Malak M. ^[2]; Heckman, Katherine ^[3]; Davis, James A. ^[1]

Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
Environmental Molecular Sciences Laboratory (EMSL), Richland, WA (United States)
USDA-Forest Service, Washington, D.C. (United States)

Here, organic carbon (OC) concentrations in subsurface sediments are typically 10 to 200 times lower than in surface soils, posing a distinct challenge for characterization. In this study, a range of chemical extractions were evaluated for extraction of natural organic matter (NOM) from two low-carbon (< 0.2%) alluvial sediments. The OC extraction efficiency followed the order pyrophosphate (PP) > NaOH > HCl, hydroxylamine hydrochloride > dithionite, water. A NOM extraction and purification scheme was developed using sequential extraction with water (MQ) and sodium pyrophosphate at pH 10 (PP), combined with purification by dialysis and solid phase extraction in order to isolate different fractions of sediment-associated NOM. Characterization of these pools of NOM for metal content and by Fourier transform infrared spectroscopy (FITR) showed that the water soluble fraction (MQ-SPE) had a higher fraction of aliphatic and carboxylic groups, while the PP-extractable NOM (PP-SPE and PP > 1kD) had higher fractions of CC groups and higher residual metals. This trend from aliphatic to more aromatic is also supported by the specific UV absorbance at 254 nm (SUVA₂₅₄) (3.5 vs 5.4 for MQ-SPE and PP-SPE, respectively) and electrospray ionization Fourier transform ion cyclotron resonance spectrometry (ESI-FTICR-MS) data which showed a greater abundance of peaks in the low O/C and high H/C region (0–0.4 O/C, 0.8–2.0 H/C) for the MQ-SPE fraction of NOM. Radiocarbon measurements yielded standard radiocarbon ages of 1020, 3095, and 9360 years BP for PP-SPE, PP > 1kD, and residual (non-extractable) OC fractions, indicating an increase in NOM stability correlated with greater metal complexation, apparent molecular weight, and aromaticity.

View Accepted Manuscript (DOE)

Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 1476582

Alternate ID(s):: OSTI ID: 1549917
OSTI ID: 1439700

Journal Information:: Organic Geochemistry, Journal Name: Organic Geochemistry Journal Issue: C Vol. 114; ISSN 0146-6380

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

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Data for: Characterization of Natural Organic Matter in Low-Carbon Sediments: Extraction and Analytical Approaches Fox, Patricia https://doi.org/10.17632/5f3hbm69w6.1 The current record is supplemented by this dataset	dataset	January 2018
Data for: Characterization of Natural Organic Matter in Low-Carbon Sediments: Extraction and Analytical Approaches Fox, Patricia https://doi.org/10.17632/5f3hbm69w6.3 The current record is supplemented by this dataset	dataset	January 2018

Characterization of natural organic matter in low-carbon sediments: Extraction and analytical approaches

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