DNA-Encoded Protein Janus Nanoparticles

Hayes, Oliver G.; McMillan, Janet R.; Lee, Byeongdu; Mirkin, Chad A.

doi:10.1021/jacs.8b05640

DNA-Encoded Protein Janus Nanoparticles

Journal Article · Sun Jun 10 00:00:00 EDT 2018 · Journal of the American Chemical Society

DOI:https://doi.org/10.1021/jacs.8b05640· OSTI ID:1475554

Hayes, Oliver G. ^[1]; McMillan, Janet R. ^[1]; Lee, Byeongdu ^[2]; Mirkin, Chad A. ^[1]

Northwestern Univ., Evanston, IL (United States). Dept. of Chemistry, and International Inst. for Nanotechnology
Argonne National Lab. (ANL), Argonne, IL (United States). X-ray Science Division

Asymmetric functionality and directional interactions, which are characteristic of noncentrosymmetric particles, such as Janus particles, present an opportunity to encode particles with properties, but also a great synthetic challenge. Here, we exploit the chemical anisotropy of proteins, and the versatile chemistry of DNA to synthesize a protein-based Janus nanoparticle comprised of two proteins encoded with sequence-specific nucleic acid domains, tethered together by an interprotein “DNA bond”. We use these novel nanoparticles to realize a new class of three-dimensional superlattice, only possible when two sides of the particle are modified with orthogonal oligonucleotide sequences. The low symmetry, intrinsic to Janus particles, enables the realization of unprecedented multicomponent nanoparticle superlattices with unique, hexagonal layered architectures. In addition, the interprotein “DNA bond” can be modulated to selectively expand the lattice in a single direction. The results presented herein not only emphasize the power of rationally designing nanoscale building blocks to create highly engineered colloidal crystals, but also establish a precedent for applications of multidomain DNA-encoded nanoparticles, especially in the field of colloidal crystallization.

View Accepted Manuscript (DOE)

Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States)

Sponsoring Organization:: National Science and Engineering Research Council of Canada; US Department of the Navy, Office of Naval Research (ONR); USDOE

Grant/Contract Number:: AC02-06CH11357

OSTI ID:: 1475554

Journal Information:: Journal of the American Chemical Society, Journal Name: Journal of the American Chemical Society Journal Issue: 29 Vol. 140; ISSN 0002-7863

Publisher:: American Chemical Society (ACS)Copyright Statement

Country of Publication:: United States

Language:: English

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