DNA-Encoded Protein Janus Nanoparticles
- Northwestern Univ., Evanston, IL (United States). Dept. of Chemistry, and International Inst. for Nanotechnology
- Argonne National Lab. (ANL), Argonne, IL (United States). X-ray Science Division
Asymmetric functionality and directional interactions, which are characteristic of noncentrosymmetric particles, such as Janus particles, present an opportunity to encode particles with properties, but also a great synthetic challenge. Here, we exploit the chemical anisotropy of proteins, and the versatile chemistry of DNA to synthesize a protein-based Janus nanoparticle comprised of two proteins encoded with sequence-specific nucleic acid domains, tethered together by an interprotein “DNA bond”. We use these novel nanoparticles to realize a new class of three-dimensional superlattice, only possible when two sides of the particle are modified with orthogonal oligonucleotide sequences. The low symmetry, intrinsic to Janus particles, enables the realization of unprecedented multicomponent nanoparticle superlattices with unique, hexagonal layered architectures. In addition, the interprotein “DNA bond” can be modulated to selectively expand the lattice in a single direction. The results presented herein not only emphasize the power of rationally designing nanoscale building blocks to create highly engineered colloidal crystals, but also establish a precedent for applications of multidomain DNA-encoded nanoparticles, especially in the field of colloidal crystallization.
- Research Organization:
- Argonne National Lab. (ANL), Argonne, IL (United States)
- Sponsoring Organization:
- National Science and Engineering Research Council of Canada; US Department of the Navy, Office of Naval Research (ONR); USDOE
- Grant/Contract Number:
- AC02-06CH11357; N00014-15-1-0043
- OSTI ID:
- 1475554
- Journal Information:
- Journal of the American Chemical Society, Vol. 140, Issue 29; ISSN 0002-7863
- Publisher:
- American Chemical Society (ACS)Copyright Statement
- Country of Publication:
- United States
- Language:
- English
Web of Science
Similar Records
Multivalent Cation-Induced Actuation of DNA-Mediated Colloidal Superlattices
DNA-mediated engineering of multicomponent enzyme crystals