Using sound pulses to solve the crystal-harvesting bottleneck

Samara, Yasmin N.; Brennan, Haley M.; McCarthy, Liam; Bollard, Mary T.; Laspina, Denise; Wlodek, Jakub M.; Campos, Stefanie L.; Natarajan, Ramya; Gofron, Kazimierz; McSweeney, Sean; Soares, Alexei S.; Leroy, Ludmila

doi:10.1107/S2059798318011506

Title: Using sound pulses to solve the crystal-harvesting bottleneck

Journal Article · Tue Oct 02 00:00:00 EDT 2018 · Acta Crystallographica. Section D. Structural Biology

DOI:https://doi.org/10.1107/S2059798318011506· OSTI ID:1475235

Samara, Yasmin N.; Brennan, Haley M.; McCarthy, Liam; Bollard, Mary T.; Laspina, Denise; Wlodek, Jakub M.; Campos, Stefanie L.; Natarajan, Ramya; Gofron, Kazimierz; McSweeney, Sean; Soares, Alexei S.;

Crystal harvesting has proven to be difficult to automate and remains the rate-limiting step for many structure-determination and high-throughput screening projects. This has resulted in crystals being prepared more rapidly than they can be harvested for X-ray data collection. Fourth-generation synchrotrons will support extraordinarily rapid rates of data acquisition, putting further pressure on the crystal-harvesting bottleneck. Here, a simple solution is reported in which crystals can be acoustically harvested from slightly modified MiTeGen In Situ -1 crystallization plates. This technique uses an acoustic pulse to eject each crystal out of its crystallization well, through a short air column and onto a micro-mesh (improving on previous work, which required separately grown crystals to be transferred before harvesting). Crystals can be individually harvested or can be serially combined with a chemical library such as a fragment library.

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Research Organization:: Brookhaven National Laboratory (BNL), Upton, NY (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES)

Grant/Contract Number:: KP1605010; SC0012704

OSTI ID:: 1475235

Alternate ID(s):: OSTI ID: 1483750

Report Number(s):: BNL-209517-2018-JAAM; ACSDAD; PII: S2059798318011506

Journal Information:: Acta Crystallographica. Section D. Structural Biology, Journal Name: Acta Crystallographica. Section D. Structural Biology Vol. 74 Journal Issue: 10; ISSN 2059-7983

Publisher:: International Union of Crystallography (IUCr)Copyright Statement

Country of Publication:: United Kingdom

Language:: English

Citation Metrics:

Cited by: 6 works

Citation information provided by
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Title: Using sound pulses to solve the crystal-harvesting bottleneck

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