Nitrous Oxide Reduction Kinetics Distinguish Bacteria Harboring Clade I NosZ from Those Harboring Clade II NosZ

Yoon, Sukhwan; Nissen, Silke; Park, Doyoung; Sanford, Robert A.; Löffler, Frank E.

doi:10.1128/AEM.00409-16

Nitrous Oxide Reduction Kinetics Distinguish Bacteria Harboring Clade I NosZ from Those Harboring Clade II NosZ

Journal Article · Fri Apr 15 00:00:00 EDT 2016 · Applied and Environmental Microbiology

DOI:https://doi.org/10.1128/AEM.00409-16· OSTI ID:1474731

Yoon, Sukhwan ^[1]; Nissen, Silke ^[2]; Park, Doyoung ^[3]; Sanford, Robert A. ^[4]; Löffler, Frank E. ^[5]

Univ. of Tennessee, Knoxville, TN (United States). Center for Environmental Biotechnology. Dept. of Microbiology; Korea Advanced Inst. of Science and Technology (KAIST), Daejeon (Korea, Republic of). Dept. of Civil and Environmental Engineering
Univ. of Tennessee, Knoxville, TN (United States). Dept. of Microbiology; Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). UT-ORNL Joint Inst. for Biological Sciences (JIBS). Biosciences Division
Korea Advanced Inst. of Science and Technology (KAIST), Daejeon (Korea, Republic of). Dept. of Civil and Environmental Engineering
Univ. of Illinois, Urbana, IL (United States). Dept. of Geology
Univ. of Tennessee, Knoxville, TN (United States). Center for Environmental Biotechnology. Dept. of Microbiology. Dept. of Civil and Environmental Engineering; Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). UT-ORNL Joint Inst. for Biological Sciences (JIBS). Biosciences Division

Bacteria capable of reduction of nitrous oxide (N₂O) to N₂ separate into clade I and clade II organisms on the basis of nos operon structures and nosZ sequence features. In this paper, to explore the possible ecological consequences of distinct nos clusters, the growth of bacterial isolates with either clade I (Pseudomonas stutzeri strain DCP-Ps1, Shewanella loihica strain PV-4) or clade II (Dechloromonas aromatica strain RCB, Anaeromyxobacter dehalogenans strain 2CP-C) nosZ with N₂O was examined. Growth curves did not reveal trends distinguishing the clade I and clade II organisms tested; however, the growth yields of clade II organisms exceeded those of clade I organisms by 1.5- to 1.8-fold. Further, whole-cell half-saturation constants (K_s s) for N₂O distinguished clade I from clade II organisms. The apparent K_s values of 0.324 ± 0.078 μM for D. aromatica and 1.34 ± 0.35 μM for A. dehalogenans were significantly lower than the values measured for P. stutzeri (35.5 ± 9.3 μM) and S. loihica (7.07 ± 1.13 μM). Genome sequencing demonstrated that Dechloromonas denitrificans possessed a clade II nosZ gene, and a measured K_s of 1.01 ± 0.18 μM for N₂O was consistent with the values determined for the other clade II organisms tested. Finally, these observations provide a plausible mechanistic basis for why the relative activity of bacteria with clade I nos operons compared to that of bacteria with clade II nos operons may control N₂O emissions and determine a soil's N₂O sink capacity.

View Accepted Manuscript (DOE)

Research Organization:: Korea Advanced Inst. of Science and Technology (KAIST), Daejeon (Korea, Republic of); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Univ. of Tennessee, Knoxville, TN (United States)

Sponsoring Organization:: National Research Foundation of Korea (NRF); USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)

Grant/Contract Number:: AC05-00OR22725; SC0006662

OSTI ID:: 1474731

Journal Information:: Applied and Environmental Microbiology, Journal Name: Applied and Environmental Microbiology Journal Issue: 13 Vol. 82; ISSN 0099-2240

Publisher:: American Society for MicrobiologyCopyright Statement

Country of Publication:: United States

Language:: English

References (58)

Nitrogen limitation on land and in the sea: How can it occur? Vitousek, PeterM.; Howarth, RobertW. Biogeochemistry, Vol. 13, Issue 2 https://doi.org/10.1007/BF00002772	journal	January 1991
Comparison of Soil Bacterial Communities of Pinus patula of Nilgiris, Western Ghats with Other Biogeographically Distant Pine Forest Clone Libraries Rohini-Kumar, M.; Osborne, Jabez W.; Saravanan, V. S. Microbial Ecology, Vol. 66, Issue 1 https://doi.org/10.1007/s00248-012-0167-y	journal	December 2012
Nitrous oxide emissions under different soil and land management conditions Henault, C.; Devis, X.; Page, S. Biology and Fertility of Soils, Vol. 26, Issue 3 https://doi.org/10.1007/s003740050368	journal	February 1998
A record of N2O and CH4 emissions and underlying soil processes of Korean rice paddies as affected by different water management practices Berger, Sina; Jang, Inyoung; Seo, Juyoung Biogeochemistry, Vol. 115, Issue 1-3 https://doi.org/10.1007/s10533-013-9837-1	journal	April 2013
The estimation of gas solubilities in salt solutions Schumpe, Adrian Chemical Engineering Science, Vol. 48, Issue 1 https://doi.org/10.1016/0009-2509(93)80291-W	journal	January 1993
Nitrous oxide and global warming Kroeze, Carolien Science of The Total Environment, Vol. 143, Issue 2-3 https://doi.org/10.1016/0048-9697(94)90457-X	journal	April 1994
An apparently allosteric effect involving N2O with the nitrous oxide reductase from Wolinella succinogenes Zhang, Chunqing; Jones, Alison M.; Hollocher, Thomas C. Biochemical and Biophysical Research Communications, Vol. 187, Issue 1 https://doi.org/10.1016/S0006-291X(05)81469-X	journal	August 1992
First practical assay for soluble nitrous oxide reductase of denitrifying bacteria and a partial kinetic characterization. Kristjansson, J. K.; Hollocher, T. C. Journal of Biological Chemistry, Vol. 255, Issue 2 https://doi.org/10.1016/S0021-9258(19)86236-1	journal	January 1980
Effect of cattle urine addition on the surface emissions and subsurface concentrations of greenhouse gases in a UK peat grassland Boon, A.; Robinson, J. S.; Chadwick, D. R. Agriculture, Ecosystems & Environment, Vol. 186 https://doi.org/10.1016/j.agee.2014.01.008	journal	March 2014
Nitrous oxide reductase Pauleta, Sofia R.; Dell’Acqua, Simone; Moura, Isabel Coordination Chemistry Reviews, Vol. 257, Issue 2 https://doi.org/10.1016/j.ccr.2012.05.026	journal	January 2013
The unprecedented nos gene cluster of Wolinella succinogenes encodes a novel respiratory electron transfer pathway to cytochrome c nitrous oxide reductase Simon, Jörg; Einsle, Oliver; Kroneck, Peter M. H. FEBS Letters, Vol. 569, Issue 1-3 https://doi.org/10.1016/j.febslet.2004.05.060	journal	June 2004
N2O and CH4 emissions from soils under conventional and no-till management practices in Goiânia (Cerrados, Brazil) Metay, Aurélie; Oliver, Robert; Scopel, Eric Geoderma, Vol. 141, Issue 1-2 https://doi.org/10.1016/j.geoderma.2007.05.010	journal	September 2007
Accumulation of nitrous oxide and depletion of oxygen in seasonally frozen soils in northern Japan – Snow cover manipulation experiments Yanai, Yosuke; Hirota, Tomoyoshi; Iwata, Yukiyoshi Soil Biology and Biochemistry, Vol. 43, Issue 9 https://doi.org/10.1016/j.soilbio.2010.06.009	journal	September 2011
N2O production rate of an enriched ammonia-oxidising bacteria culture exponentially correlates to its ammonia oxidation rate Law, Yingyu; Ni, Bing-Jie; Lant, Paul Water Research, Vol. 46, Issue 10 https://doi.org/10.1016/j.watres.2012.03.043	journal	June 2012
Effects of Agriculture upon the Air Quality and Climate: Research, Policy, and Regulations Aneja, Viney P.; Schlesinger, William H.; Erisman, Jan Willem Environmental Science & Technology, Vol. 43, Issue 12 https://doi.org/10.1021/es8024403	journal	June 2009
Sources of nitrous oxide in soils Bremner, John M. Nutrient Cycling in Agroecosystems, Vol. 49, Issue 1/3, p. 7-16 https://doi.org/10.1023/A:1009798022569	journal	January 1997
Relative contributions of greenhouse gas emissions to global warming Lashof, Daniel A.; Ahuja, Dilip R. Nature, Vol. 344, Issue 6266 https://doi.org/10.1038/344529a0	journal	April 1990
Methane and nitrous oxide fluxes in native, fertilized and cultivated grasslands Mosier, A.; Schimel, D.; Valentine, D. Nature, Vol. 350, Issue 6316 https://doi.org/10.1038/350330a0	journal	March 1991
A novel type of catalytic copper cluster in nitrous oxide reductase Cambillau, Christian; Brown, Kieron; Tegoni, Mariella Nature Structural Biology, Vol. 7, Issue 3, p. 191-195 https://doi.org/10.1038/73288	journal	March 2000
Bacterial gene abundances as indicators of greenhouse gas emission in soils Morales, Sergio E.; Cosart, Theodore; Holben, William E. The ISME Journal, Vol. 4, Issue 6 https://doi.org/10.1038/ismej.2010.8	journal	February 2010
The unaccounted yet abundant nitrous oxide-reducing microbial community: a potential nitrous oxide sink Jones, Christopher M.; Graf, Daniel RH; Bru, David The ISME Journal, Vol. 7, Issue 2 https://doi.org/10.1038/ismej.2012.125	journal	November 2012
Denitrification versus respiratory ammonification: environmental controls of two competing dissimilatory NO3−/NO2− reduction pathways in Shewanella loihica strain PV-4 Yoon, Sukhwan; Cruz-García, Claribel; Sanford, Robert The ISME Journal, Vol. 9, Issue 5 https://doi.org/10.1038/ismej.2014.201	journal	October 2014
Recently identified microbial guild mediates soil N2O sink capacity Jones, Christopher M.; Spor, Ayme; Brennan, Fiona P. Nature Climate Change, Vol. 4, Issue 9 https://doi.org/10.1038/nclimate2301	journal	July 2014
Climate change impacts of US reactive nitrogen Pinder, R. W.; Davidson, E. A.; Goodale, C. L. Proceedings of the National Academy of Sciences, Vol. 109, Issue 20 https://doi.org/10.1073/pnas.1114243109	journal	April 2012
Unexpected nondenitrifier nitrous oxide reductase gene diversity and abundance in soils Sanford, R. A.; Wagner, D. D.; Wu, Q. Proceedings of the National Academy of Sciences, Vol. 109, Issue 48 https://doi.org/10.1073/pnas.1211238109	journal	November 2012
Ammonia oxidation pathways and nitrifier denitrification are significant sources of N2O and NO under low oxygen availability Zhu, X.; Burger, M.; Doane, T. A. Proceedings of the National Academy of Sciences, Vol. 110, Issue 16 https://doi.org/10.1073/pnas.1219993110	journal	April 2013
Experimental Tests for an Evolutionary Trade‐Off between Growth Rate and Yield in E. coli Novak, Maja; Pfeiffer, Thomas; Lenski, Richard E. The American Naturalist, Vol. 168, Issue 2 https://doi.org/10.1086/506527	journal	August 2006
Stratospheric ozone depletion due to nitrous oxide: influences of other gases Portmann, R. W.; Daniel, J. S.; Ravishankara, A. R. Philosophical Transactions of the Royal Society B: Biological Sciences, Vol. 367, Issue 1593 https://doi.org/10.1098/rstb.2011.0377	journal	May 2012
Biological sources and sinks of nitrous oxide and strategies to mitigate emissions Thomson, Andrew J.; Giannopoulos, Georgios; Pretty, Jules Philosophical Transactions of the Royal Society B: Biological Sciences, Vol. 367, Issue 1593 https://doi.org/10.1098/rstb.2011.0415	journal	May 2012
Three transcription regulators of the Nss family mediate the adaptive response induced by nitrate, nitric oxide or nitrous oxide in W olinella succinogenes : Role of three Nss-type regulators in Kern, Melanie; Simon, Jörg Environmental Microbiology, Vol. 18, Issue 9 https://doi.org/10.1111/1462-2920.13060	journal	October 2015
Strong pH influence on N ₂ O and CH ₄ fluxes from forested organic soils Weslien, P.; Kasimir Klemedtsson, Å.; Börjesson, G. European Journal of Soil Science, Vol. 60, Issue 3 https://doi.org/10.1111/j.1365-2389.2009.01123.x	journal	June 2009
Soils, a sink for N ₂ O? A review Chapuis-Lardy, Lydie; Wrage, Nicole; Metay, AurÉLie Global Change Biology, Vol. 13, Issue 1 https://doi.org/10.1111/j.1365-2486.2006.01280.x	journal	January 2007
Drought turns a Central European Norway spruce forest soil from an N ₂ O source to a transient N ₂ O sink Goldberg, Stefanie Daniela; Gebauer, Gerhard Global Change Biology, Vol. 15, Issue 4 https://doi.org/10.1111/j.1365-2486.2008.01752.x	journal	April 2009
Importance of denitrifiers lacking the genes encoding the nitrous oxide reductase for N2O emissions from soil: ROLE OF DENITRIFIER DIVERSITY FOR N2O FLUXES Philippot, Laurent; Andert, Janet; Jones, Christopher M. Global Change Biology, Vol. 17, Issue 3 https://doi.org/10.1111/j.1365-2486.2010.02334.x	journal	November 2010
Nitrous oxide reductase from denitrifying. Pseudomonas perfectomarina Purification and properties of a novel multicopper enzyme Coyle, Catherine L.; Zumft, Walter G.; Kroneck, Peter M. H. European Journal of Biochemistry, Vol. 153, Issue 3 https://doi.org/10.1111/j.1432-1033.1985.tb09324.x	journal	December 1985
Response and adaptation of different methanotrophic bacteria to low methane mixing ratios Knief, Claudia; Dunfield, Peter F. Environmental Microbiology, Vol. 7, Issue 9 https://doi.org/10.1111/j.1462-2920.2005.00814.x	journal	September 2005
Denitrification gene pools, transcription and kinetics of NO, N2O and N2 production as affected by soil pH: Gene transcription, gas kinetics as affected by soil pH Liu, Binbin; Mørkved, Pål Tore; Frostegård, Åsa FEMS Microbiology Ecology, Vol. 72, Issue 3 https://doi.org/10.1111/j.1574-6941.2010.00856.x	journal	February 2010
Nitrous oxide production in soil isolates of nitrate-ammonifying bacteria: Nitrous oxide production in nitrate-ammonifying bacteria Stremińska, Marta A.; Felgate, Heather; Rowley, Gary Environmental Microbiology Reports, Vol. 4, Issue 1 https://doi.org/10.1111/j.1758-2229.2011.00302.x	journal	October 2011
Global Consequences of Land Use Foley, J. A. Science, Vol. 309, Issue 5734 https://doi.org/10.1126/science.1111772	journal	July 2005
Nitrous Oxide (N2O): The Dominant Ozone-Depleting Substance Emitted in the 21st Century Ravishankara, A. R.; Daniel, J. S.; Portmann, R. W. Science, Vol. 326, Issue 5949 https://doi.org/10.1126/science.1176985	journal	August 2009
Cell biology and molecular basis of denitrification. Zumft, W. G. Microbiology and molecular biology reviews : MMBR, Vol. 61, Issue 4 https://doi.org/10.1128/.61.4.533-616.1997	journal	January 1997
Nitrite Control over Dissimilatory Nitrate/Nitrite Reduction Pathways in Shewanella loihica Strain PV-4 Yoon, Sukhwan; Sanford, Robert A.; Löffler, Frank E. Applied and Environmental Microbiology, Vol. 81, Issue 10 https://doi.org/10.1128/AEM.00688-15	journal	March 2015
Unique Ecophysiology among U(VI)-Reducing Bacteria as Revealed by Evaluation of Oxygen Metabolism in Anaeromyxobacter dehalogenans Strain 2CP-C Thomas, S. H.; Sanford, R. A.; Amos, B. K. Applied and Environmental Microbiology, Vol. 76, Issue 1 https://doi.org/10.1128/AEM.01854-09	journal	November 2009
Insights into the Effect of Soil pH on N2O and N2 Emissions and Denitrifier Community Size and Activity Cuhel, J.; Simek, M.; Laughlin, R. J. Applied and Environmental Microbiology, Vol. 76, Issue 6 https://doi.org/10.1128/AEM.02484-09	journal	January 2010
Changes in Denitrifier Abundance, Denitrification Gene mRNA Levels, Nitrous Oxide Emissions, and Denitrification in Anoxic Soil Microcosms Amended with Glucose and Plant Residues Henderson, S. L.; Dandie, C. E.; Patten, C. L. Applied and Environmental Microbiology, Vol. 76, Issue 7 https://doi.org/10.1128/AEM.02993-09	journal	February 2010
Shewanella spp. Use Acetate as an Electron Donor for Denitrification but Not Ferric Iron or Fumarate Reduction Yoon, Sukhwan; Sanford, Robert A.; Löffler, Frank E. Applied and Environmental Microbiology, Vol. 79, Issue 8 https://doi.org/10.1128/AEM.03872-12	journal	February 2013
Kinetic Explanation for Accumulation of Nitrite, Nitric Oxide, and Nitrous Oxide During Bacterial Denitrification † Betlach, Michael R.; Tiedje, James M. Applied and Environmental Microbiology, Vol. 42, Issue 6 https://doi.org/10.1128/AEM.42.6.1074-1084.1981	journal	January 1981
Inability of Pseudomonas stutzeri denitrification mutants with the phenotype of Pseudomonas aeruginosa to grow in nitrous oxide. Bryan, B. A.; Jeter, R. M.; Carlson, C. A. Applied and Environmental Microbiology, Vol. 50, Issue 5 https://doi.org/10.1128/AEM.50.5.1301-1303.1985	journal	January 1985
rRNA Operon Copy Number Reflects Ecological Strategies of Bacteria Klappenbach, J. A.; Dunbar, J. M.; Schmidt, T. M. Applied and Environmental Microbiology, Vol. 66, Issue 4 https://doi.org/10.1128/AEM.66.4.1328-1333.2000	journal	April 2000
Nitrous Oxide Reductase Genes (nosZ) of Denitrifying Microbial Populations in Soil and the Earthworm Gut Are Phylogenetically Similar Horn, M. A.; Drake, H. L.; Schramm, A. Applied and Environmental Microbiology, Vol. 72, Issue 2 https://doi.org/10.1128/AEM.72.2.1019-1026.2006	journal	February 2006
Identifying the Dominant Soil Bacterial Taxa in Libraries of 16S rRNA and 16S rRNA Genes Janssen, P. H. Applied and Environmental Microbiology, Vol. 72, Issue 3 https://doi.org/10.1128/AEM.72.3.1719-1728.2006	journal	March 2006
Symbiotic Bradyrhizobium japonicum Reduces N2O Surrounding the Soybean Root System via Nitrous Oxide Reductase Sameshima-Saito, R.; Chiba, K.; Hirayama, J. Applied and Environmental Microbiology, Vol. 72, Issue 4 https://doi.org/10.1128/AEM.72.4.2526-2532.2006	journal	April 2006
Energetics of bacterial growth: balance of anabolic and catabolic reactions. Russell, J. B.; Cook, G. M. Microbiological reviews, Vol. 59, Issue 1 https://doi.org/10.1128/MMBR.59.1.48-62.1995	journal	January 1995
Kinetic Explanation for Accumulation of Nitrite, Nitric Oxide, and Nitrous Oxide During Bacterial Denitrification † Betlach, Michael R.; Tiedje, James M. Applied and Environmental Microbiology, Vol. 42, Issue 6 https://doi.org/10.1128/aem.42.6.1074-1084.1981	journal	January 1981
Inability of Pseudomonas stutzeri denitrification mutants with the phenotype of Pseudomonas aeruginosa to grow in nitrous oxide. Bryan, B. A.; Jeter, R. M.; Carlson, C. A. Applied and Environmental Microbiology, Vol. 50, Issue 5 https://doi.org/10.1128/aem.50.5.1301-1303.1985	journal	January 1985
Detecting Nitrous Oxide Reductase (nosZ) Genes in Soil Metagenomes: Method Development and Implications for the Nitrogen Cycle Orellana, L. H.; Rodriguez-R, L. M.; Higgins, S. mBio, Vol. 5, Issue 3 https://doi.org/10.1128/mBio.01193-14	journal	June 2014
Cell biology and molecular basis of denitrification Zumft, W. G. Microbiology and Molecular Biology Reviews, Vol. 61, Issue 4 https://doi.org/10.1128/mmbr.61.4.533-616.1997	journal	December 1997
Energetics of bacterial growth: balance of anabolic and catabolic reactions. Russell, J. B.; Cook, G. M. Microbiological reviews, Vol. 59, Issue 1 https://doi.org/10.1128/mr.59.1.48-62.1995	journal	January 1995

Cited By (11)

O2 versus N2O respiration in a continuous microbial enrichment Conthe, Monica; Parchen, Camiel; Stouten, Gerben Applied Microbiology and Biotechnology, Vol. 102, Issue 20 https://doi.org/10.1007/s00253-018-9247-3	journal	July 2018
Nitrous oxide emissions from biofilm processes for wastewater treatment Sabba, Fabrizio; Terada, Akihiko; Wells, George Applied Microbiology and Biotechnology, Vol. 102, Issue 22 https://doi.org/10.1007/s00253-018-9332-7	journal	September 2018
Life on N2O: deciphering the ecophysiology of N2O respiring bacterial communities in a continuous culture Conthe, Monica; Wittorf, Lea; Kuenen, J. Gijs The ISME Journal, Vol. 12, Issue 4 https://doi.org/10.1038/s41396-018-0063-7	journal	February 2018
Ecological and physiological implications of nitrogen oxide reduction pathways on greenhouse gas emissions in agroecosystems Yoon, Sukhwan; Song, Bongkeun; Phillips, Rebecca L. FEMS Microbiology Ecology, Vol. 95, Issue 6 https://doi.org/10.1093/femsec/fiz066	journal	May 2019
Resolving N ₂ O respiration pathways: a tale of two NosZ clades : Resolving N Stein, Lisa Y. Environmental Microbiology, Vol. 19, Issue 12 https://doi.org/10.1111/1462-2920.13969	journal	November 2017
Carbon-driven enrichment of the crucial nitrate-reducing bacteria in limed peat soil microcosms Zhu, Y.; Zhang, X.; Wu, X. Letters in Applied Microbiology, Vol. 65, Issue 2 https://doi.org/10.1111/lam.12756	journal	July 2017
Nitrous Oxide Reduction by an Obligate Aerobic Bacterium, Gemmatimonas aurantiaca Strain T-27 Park, Doyoung; Kim, Hayeon; Yoon, Sukhwan Applied and Environmental Microbiology, Vol. 83, Issue 12 https://doi.org/10.1128/aem.00502-17	journal	April 2017
Draft Genome Sequence of Azospira sp. Strain I13, a Nitrous Oxide-Reducing Bacterium Harboring Clade II Type nosZ Suenaga, Toshikazu; Aoyagi, Tomo; Hosomi, Masaaki Genome Announcements, Vol. 6, Issue 20 https://doi.org/10.1128/genomea.00414-18	journal	May 2018
Predominance and high diversity of genes associated to denitrification in metagenomes of subantarctic coastal sediments exposed to urban pollution Calderoli, Priscila A.; Espínola, Fernando J.; Dionisi, Hebe M. PLOS ONE, Vol. 13, Issue 11 https://doi.org/10.1371/journal.pone.0207606	journal	November 2018
Biokinetic Characterization and Activities of N2O-Reducing Bacteria in Response to Various Oxygen Levels Suenaga, Toshikazu; Riya, Shohei; Hosomi, Masaaki Frontiers in Microbiology, Vol. 9 https://doi.org/10.3389/fmicb.2018.00697	journal	April 2018
New Breeding Techniques for Greenhouse Gas (GHG) Mitigation: Plants May Express Nitrous Oxide Reductase Demone, Jordan; Wan, Shen; Nourimand, Maryam Climate, Vol. 6, Issue 4 https://doi.org/10.3390/cli6040080	journal	September 2018

Similar Records

Variable Inhibition of Nitrous Oxide Reduction in Denitrifying Bacteria by Different Forms of Methanobactin

Journal Article · Sun Mar 13 20:00:00 EDT 2022 · Applied and Environmental Microbiology · OSTI ID:1865104

A new primer set for Clade I nosZ that recovers genes from a broader range of taxa

Journal Article · Sat Feb 13 19:00:00 EST 2021 · Biology and Fertility of Soils · OSTI ID:1846010

Related Subjects

54 ENVIRONMENTAL SCIENCES
59 BASIC BIOLOGICAL SCIENCES

Nitrous Oxide Reduction Kinetics Distinguish Bacteria Harboring Clade I NosZ from Those Harboring Clade II NosZ

Citation Formats

References (58)

Cited By (11)

Similar Records

Related Subjects