Clay Flocculation Effect on Microbial Community Composition in Water and Sediment

Chen, Chunyi; Pan, Gang; Shi, Wenqing; Xu, Feng; Techtmann, Stephen M.; Pfiffner, Susan M.; Hazen, Terry C.

doi:10.3389/fenvs.2018.00060

Clay Flocculation Effect on Microbial Community Composition in Water and Sediment

Journal Article · Mon Jun 25 00:00:00 EDT 2018 · Frontiers in Environmental Science

DOI:https://doi.org/10.3389/fenvs.2018.00060· OSTI ID:1474524

Chen, Chunyi ^[1]; Pan, Gang ^[2]; Shi, Wenqing ^[2]; Xu, Feng ^[2]; Techtmann, Stephen M. ^[3]; Pfiffner, Susan M. ^[4]; Hazen, Terry C. ^[4]

Univ. of Tennessee, Knoxville, TN (United States)
Chinese Academy of Sciences (CAS), Beijing (China)
Univ. of Tennessee, Knoxville, TN (United States); Michigan Technological Univ., Houghton, MI (United States)
Univ. of Tennessee, Knoxville, TN (United States); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)

Clay-based flocculation techniques have been developed to mitigate harmful algal blooms; however, the potential ecological impacts on the microbial community are poorly understood. In this study, chemical measurements were combined with 16S rRNA sequencing to characterize the microbial community response to different flocculation techniques, including controls, clay flocculation, clay flocculation with zeolite, and clay flocculation with O₂ added zeolite capping. Sediment bacterial biomass measured by PLFA were not significantly altered by the various flocculation techniques used. However, 16S rRNA sequencing revealed differences in water microbial community structure between treatments with and without zeolite capping. The differences were related to significant reductions of total nitrogen (TN), total phosphorus (TP) and ammonia (NH₄₊) concentration and increase of nitrate (NO_3-) concentration in zeolite and O₂ loaded zeolite capping. The relative abundance of ammonia oxidizing bacteria increased four-fold in zeolite capping microcosms, suggesting zeolite promoted absorbed ammonia removal in the benthic zone. Zeolite-capping promoted bacteria nitrogen cycling activities at the water-sediment interface. Potential pathogens that are usually adapted to eutrophic water bodies were reduced after clay flocculation. This study demonstrated clay flocculation did not decrease bacterial populations overall and may reduce regulatory indicators and pathogenic contaminants in water. Zeolite capping may also help prevent nutrients from being released back into the water thus preventing additional algal blooms.

View Accepted Manuscript (DOE)

Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE

Grant/Contract Number:: AC05-00OR22725

OSTI ID:: 1474524

Journal Information:: Frontiers in Environmental Science, Journal Name: Frontiers in Environmental Science Journal Issue: n/a Vol. 6; ISSN 2296-665X

Publisher:: Frontiers Research FoundationCopyright Statement

Country of Publication:: United States

Language:: English

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