A new symbiotic nanoarchaeote (Candidatus Nanoclepta minutus) and its host (Zestosphaera tikiterensis gen. nov., sp. nov.) from a New Zealand hot spring

St. John, Emily; Liu, Yitai; Podar, Mircea; Stott, Matthew B.; Meneghin, Jennifer; Chen, Zhiqiang; Lagutin, Kirill; Mitchell, Kevin; Reysenbach, Anna-Louise

doi:10.1016/j.syapm.2018.08.005

A new symbiotic nanoarchaeote (Candidatus Nanoclepta minutus) and its host (Zestosphaera tikiterensis gen. nov., sp. nov.) from a New Zealand hot spring

Journal Article · Thu Aug 16 00:00:00 EDT 2018 · Systematic and Applied Microbiology

DOI:https://doi.org/10.1016/j.syapm.2018.08.005· OSTI ID:1470848

St. John, Emily ^[1]; Liu, Yitai ^[1]; ^[2]; ^[3]; Meneghin, Jennifer ^[1]; Chen, Zhiqiang ^[1]; Lagutin, Kirill ^[4]; Mitchell, Kevin ^[4]; Reysenbach, Anna-Louise ^[1]

Portland State Univ., Portland, OR (United States)
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Univ. of Tennessee, Knoxville, TN (United States)
Univ. of Canterbury, Christchurch (New Zealand)
Callaghan Innovation, Lower Hutt (New Zealand)

Three thermophilic Nanoarchaeota-Crenarchaeota symbiotic systems have been described. We obtained another stable anaerobic enrichment culture at 80 °C, pH 6.0 from a New Zealand hot spring. The nanoarchaeote (Ncl-1) and its host (NZ3^T) were isolated in co-culture and their genomes assembled. The small (~200 nm) flagellated cocci were often attached to larger cocci. Based on 16S rRNA gene similarity (88.4%) and average amino acid identity (52%), Ncl-1 is closely related to Candidatus Nanopusillus acidilobi. Their genomes both encode for archaeal flagella and partial glycolysis and gluconeogenesis pathways, but lack ATP synthase genes. Like Nanoarchaeum equitans, Ncl-1 has a CRISPR-Cas system. Ncl-1 also relies on its crenarchaeotal host for most of its biosynthetic needs. The host NZ3^T was isolated and grows on proteinaceous substrates but not on sugars, alcohols, or fatty acids. NZ3^T requires thiosulfate and grows best at 82 °C, pH 6.0. NZ3^T is most closely related to the Desulfurococcaceae, Ignisphaera aggregans (~92% 16S rRNA gene sequence similarity, 45% AAI). Based on phylogenetic, physiological and genomic data, Ncl-1 and NZ3T represent novel genera in the Nanoarchaeota and the Desulfurococcaceae, respectively, with the proposed names Candidatus Nanoclepta minutus and Zestosphaera tikiterensis gen. nov., sp. nov., type strain NZ3^T (=DSMZ 107634^T = OCM 1213^T).

View Accepted Manuscript (DOE)

Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE

Grant/Contract Number:: AC05-00OR22725

OSTI ID:: 1470848

Journal Information:: Systematic and Applied Microbiology, Journal Name: Systematic and Applied Microbiology Journal Issue: 1 Vol. 42; ISSN 0723-2020

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

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