Dual Role of Humic Substances As Electron Donor and Shuttle for Dissimilatory Iron Reduction

Stern, Noah; Mejia, Jacqueline; He, Shaomei; Yang, Yu; Ginder-Vogel, Matthew; Roden, Eric E.

doi:10.1021/acs.est.7b06574

Dual Role of Humic Substances As Electron Donor and Shuttle for Dissimilatory Iron Reduction

Journal Article · Mon Apr 16 00:00:00 EDT 2018 · Environmental Science and Technology

DOI:https://doi.org/10.1021/acs.est.7b06574· OSTI ID:1469803

Stern, Noah ^[1]; Mejia, Jacqueline ^[2]; He, Shaomei ^[3]; Yang, Yu ^[4]; ^[2]; ^[5]

Univ. of Wisconsin, Madison, WI (United States). Dept. of Civil and Environmental Engineering, Environmental Chemistry and Technology Program; Unniversity of Wisconsin Department of Geoscience
Univ. of Wisconsin, Madison, WI (United States). Dept. of Civil and Environmental Engineering, Environmental Chemistry and Technology Program
Univ. of Wisconsin, Madison, WI (United States). Dept. of Geoscience, Dept. of Bacteriology
Univ. of Nevada, Reno, NV (United States). Dept. of Civil and Environmental Engineering
Univ. of Wisconsin, Madison, WI (United States). Dept. of Geoscience

Dissimilatory iron-reducing bacteria (DIRB) are known to use humic substances (HS) as electron shuttles for dissimilatory iron reduction (DIR) by transferring electrons to HS-quinone moieties, which in turn rapidly reduce Fe(III) oxides. However, the potential for HS to serve as a source of organic carbon (OC) that can donate electrons for DIR is unknown. We studied whether humic acids (HA) and humins (HM) recovered from peat soil by sodium pyrophosphate extraction could serve as both electron shuttles and electron donors for DIR by freshwater sediment microorganisms. Both HA and HM served as electron shuttles in cultures amended with glucose. However, only HA served as an electron donor for DIR. Metagenomes from HA-containing cultures had an overrepresentation of genes involved in polysaccharide and to a lesser extent aromatic compound degradation, suggesting complex OC metabolism. Genomic searches for the porin-cytochrome complex involved in DIR resulted in matches to Ignavibacterium/Melioribacter, DIRB capable of polymeric OC metabolism. These results indicate that such taxa may have played a role in both DIR and decomposition of complex OC. Our results suggest that decomposition of HS coupled to DIR and other anaerobic pathways could play an important role in soil and sediment OC metabolism.

View Accepted Manuscript (DOE)

Research Organization:: Univ. of Wisconsin, Madison, WI (United States); Univ. of Wisconsin-Madison, Madison, WI (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)

Contributing Organization:: SBR Scientific Focus Area at the Pacific Northwest National Laboratory (PNNL)

Grant/Contract Number:: SC0016217

OSTI ID:: 1469803

Alternate ID(s):: OSTI ID: 1480447

Journal Information:: Environmental Science and Technology, Journal Name: Environmental Science and Technology Journal Issue: 10 Vol. 52; ISSN 0013-936X

Publisher:: American Chemical Society (ACS)Copyright Statement

Country of Publication:: United States

Language:: English

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