Skip to main content
OSTI.GOVU.S. Department of Energy Office of Scientific and Technical Information
U.S. Department of Energy
Office of Scientific and Technical Information
 

Transcriptional Responses of the Bdtf1-Deletion Mutant to the Phytoalexin Brassinin in the Necrotrophic Fungus Alternaria brassicicola

Journal Article · · Molecules
 [1];  [2];  [1];  [3];  [2];  [1];  [1]
  1. Korea Research Institute of Bioscience and Biotechnology, Chungbuk (Korea)
  2. USDOE Joint Genome Institute (JGI), Walnut Creek, CA (United States)
  3. Chonnam National Univ., Gwangju (Korea)
+ Show Author Affiliations
Brassica species produce the antifungal indolyl compounds brassinin and its derivatives, during microbial infection. The fungal pathogen Alternaria brassicicola detoxifies brassinin and possibly its derivatives. This ability is an important property for the successful infection of brassicaceous plants. Previously, we identified a transcription factor, Bdtf1, essential for the detoxification of brassinin and full virulence. To discover genes that encode putative brassinin-digesting enzymes, we compared gene expression profiles between a mutant strain of the transcription factor and wild-type A. brassicicola under two different experimental conditions. A total of 170 and 388 genes were expressed at higher levels in the mutants than the wild type during the infection of host plants and saprophytic growth in the presence of brassinin, respectively. In contrast, 93 and 560 genes were expressed, respectively, at lower levels in the mutant than the wild type under the two conditions. Fifteen of these genes were expressed at lower levels in the mutant than in the wild type under both conditions. These genes were assumed to be important for the detoxification of brassinin and included Bdtf1 and 10 putative enzymes. In conclusion, this list of genes provides a resource for the discovery of enzyme-coding genes important in the chemical modification of brassinin.
Research Organization:
Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
Sponsoring Organization:
Genomics Division; USDOE
OSTI ID:
1469146
Report Number(s):
LBNL--177995; ir:177995
Journal Information:
Molecules, Journal Name: Molecules Journal Issue: 8 Vol. 19; ISSN MOLEFW; ISSN 1420-3049
Publisher:
MDPICopyright Statement
Country of Publication:
United States
Language:
English

References (34)

Detoxification of the cruciferous phytoalexin brassinin in Sclerotinia sclerotiorum requires an inducible glucosyltransferase journal October 2004
Metabolism and detoxification of phytoalexins and analogs by phytopathogenic fungi journal February 2005
Detoxification of cruciferous phytoalexins in Botrytis cinerea: Spontaneous dimerization of a camalexin metabolite journal February 2011
Synthetic Inhibitors of the Fungal Detoxifying Enzyme Brassinin Oxidase Based on the Phytoalexin Camalexin Scaffold journal March 2009
The phytoalexins from cultivated and wild crucifers: Chemistry and biology journal January 2011
Isolation of three novel sulphur-containing phytoalexins from the chinese cabbage Brassica campestris L. ssp. pekinensis(cruciferae) journal January 1986
TopHat: discovering splice junctions with RNA-Seq journal March 2009
The Genome Portal of the Department of Energy Joint Genome Institute journal November 2011
Characterization of the Gene Encoding Pisatin Demethylase ( FoPDA 1) in Fusarium oxysporum journal December 2011
The Bdtf1 Gene in Alternaria brassicicola Is Important in Detoxifying Brassinin and Maintaining Virulence on Brassica Species journal December 2013
A Zinc-Finger-Family Transcription Factor, AbVf19 , Is Required for the Induction of a Gene Subset Important for Virulence in Alternaria brassicicola journal April 2012
A High Throughput Targeted Gene Disruption Method for Alternaria brassicicola Functional Genomics Using Linear Minimal Element (LME) Constructs journal January 2006
Mode of Action of the Arabidopsis thaliana Phytoalexin Camalexin and Its Role in Arabidopsis-Pathogen Interactions journal January 1996
Transcriptional responses to exposure to the brassicaceous defence metabolites camalexin and allyl-isothiocyanate in the necrotrophic fungus Alternaria brassicicola journal March 2007
Differential protein expression in response to the phytoalexin brassinin allows the identification of molecular targets in the phytopathogenic fungus Alternaria brassicicola: Identification of phytoalexin molecular targets journal November 2011
Identification of novel virulence factors associated with signal transduction pathways in Alternaria brassicicola journal June 2009
In vitro antifungal activity of brassinin, camalexin and two isothiocyanates against the crucifer pathogens Alternaria brassicicola and Alternaria brassicae journal April 2007
Substrate specificity and inhibition of brassinin hydrolases, detoxifying enzymes from the plant pathogens Leptosphaeria maculans and Alternaria brassicicola: Brassinin hydrolases journal November 2009
Fungal-specific transcription factor AbPf2 activates pathogenicity in Alternaria brassicicola journal May 2013
One Enzyme Makes a Fungal Pathogen, But Not a Saprophyte, Virulent on a New Host Plant journal October 1989
Improving RNA-Seq expression estimates by correcting for fragment bias journal January 2011
Diverse Lifestyles and Strategies of Plant Pathogenesis Encoded in the Genomes of Eighteen Dothideomycetes Fungi journal December 2012
The phytopathogenic fungus Alternaria brassicicola: Phytotoxin production and phytoalexin elicitation journal February 2009
Infection of Arabidopsis with a necrotrophic pathogen, Botrytis cinerea, elicits various defense responses but does not induce systemic acquired resistance (SAR) journal January 2002
Transcript assembly and quantification by RNA-Seq reveals unannotated transcripts and isoform switching during cell differentiation journal May 2010
Characterization of the Gene Encoding Pisatin Demethylase ( FoPDA 1) in Fusarium oxysporum journal December 2011
The Bdtf1 Gene in Alternaria brassicicola Is Important in Detoxifying Brassinin and Maintaining Virulence on Brassica Species journal December 2013
A Zinc-Finger-Family Transcription Factor, AbVf19 , Is Required for the Induction of a Gene Subset Important for Virulence in Alternaria brassicicola journal April 2012
A High Throughput Targeted Gene Disruption Method for Alternaria brassicicola Functional Genomics Using Linear Minimal Element (LME) Constructs journal January 2006
Mode of Action of the Arabidopsis thaliana Phytoalexin Camalexin and Its Role in Arabidopsis-Pathogen Interactions journal January 1996
Cell wall integrity and high osmolarity glycerol pathways are required for adaptation of Alternaria brassicicola to cell wall stress caused by brassicaceous indolic phytoalexins: Camalexin activation of HOG and CWI pathways journal September 2010
Brassinin oxidase, a fungal detoxifying enzyme to overcome a plant defense - purification, characterization and inhibition: Brassinin oxidase, a fungal detoxifying enzyme journal June 2008
Ultrafast and memory-efficient alignment of short DNA sequences to the human genome journal January 2009
Transcription Factor Amr1 Induces Melanin Biosynthesis and Suppresses Virulence in Alternaria brassicicola journal October 2012

Cited By (6)

Validation of reference genes for normalization of gene expression by qRT-PCR in a resveratrol-producing entophytic fungus (Alternaria sp. MG1) journal November 2016
A Pectate Lyase-Coding Gene Abundantly Expressed during Early Stages of Infection Is Required for Full Virulence in Alternaria brassicicola journal May 2015
Responses of the Necrotrophic Fungus Alternaria brassisicola to the Indolic Phytoalexin Brassinin journal January 2021
Deciphering the Role of Phytoalexins in Plant-Microorganism Interactions and Human Health journal November 2014
Phytoalexins: Current Progress and Future Prospects journal February 2015
Phytoalexins: Current Progress and Future Prospects book March 2015

Figures / Tables (6)

Figure 1(p. 3)figure Figure 1
Table 1(p. 5)table Table 1
Table 2(p. 6)table Table 2
Table 3(p. 6)table Table 3
Figure 2(p. 8)figure Figure 2
Table 4(p. 9)table Table 4

Similar Records

Transcription Factor Amr1 Induces Melanin Biosynthesis and Suppresses Virulence in Alternaria brassicicola
Journal Article · Tue May 01 00:00:00 EDT 2012 · PLoS Pathogens · OSTI ID:1165992
Fungal-specific transcription factor AbPf2 activates pathogenicity in Alternaria brassicicola
Journal Article · Fri May 24 00:00:00 EDT 2013 · The Plant Journal · OSTI ID:1241206

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
GEO Series Accession No. GSE59195
RNA-seq
brassinin
gene expression profiles
necrotrophic fungus
phytoalexin
phytoalexin detoxification