Evaluation of non-invasive biomonitoring of 2,4-Dichlorophenoxyacetic acid (2,4-D) in saliva

Carver, Zana A.; Han, Alice A.; Timchalk, Charles; Weber, Thomas J.; Tyrrell, Kimberly J.; Sontag, Ryan L.; Luders, Teresa; Chrisler, William B.; Weitz, Karl K.; Smith, Jordan N.

doi:10.1016/j.tox.2018.08.003

Evaluation of non-invasive biomonitoring of 2,4-Dichlorophenoxyacetic acid (2,4-D) in saliva

Journal Article · Thu Aug 16 00:00:00 EDT 2018 · Toxicology

DOI:https://doi.org/10.1016/j.tox.2018.08.003· OSTI ID:1468952

Carver, Zana A. ^[1]; Han, Alice A. ^[2]; Timchalk, Charles ^[2]; Weber, Thomas J. ^[2]; Tyrrell, Kimberly J. ^[2]; ^[2]; Luders, Teresa ^[2]; ^[2]; Weitz, Karl K. ^[2]; ^[2]

Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Columbia Basin College, Pasco, WA (United States)
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)

Here, the objective of this study was to evaluate the potential for non-invasive biomonitoring of 2,4-Dichlorophenoxyacetic acid (2,4-D) in saliva. Using an in vitro rat salivary gland epithelial cell (SGEC) system, a collection of experiments investigating chemical protein binding, temporal and directional transport, as well as competitive transport with para-aminohippuric acid (PAH), a substrate for renal organic anion transporters, was conducted to identify cellular transport parameters required to computationally model salivary transport of 2,4-D. Additionally, a physiological protein gradient was implemented to mimic physiologically relevant concentrations of protein in rat plasma and saliva, and under these conditions the transfer of 2,4-D was markedly slower, driven by increased protein binding (i.e. reduced free 2,4-D species available to cross salivary barrier).

View Accepted Manuscript (DOE)

Research Organization:: Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)

Sponsoring Organization:: USDOE

Grant/Contract Number:: AC05-76RL01830

OSTI ID:: 1468952

Alternate ID(s):: OSTI ID: 1503498

Report Number(s):: PNNL-SA--135310; PII: S0300483X18302324

Journal Information:: Toxicology, Journal Name: Toxicology Journal Issue: 0 Vol. 0; ISSN 0300-483X

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

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2
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60 APPLIED LIFE SCIENCES
Biological modeling
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In vitro and alternatives

Evaluation of non-invasive biomonitoring of 2,4-Dichlorophenoxyacetic acid (2,4-D) in saliva

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