Evaporation-induced monolayer compression improves droplet interface bilayer formation using unsaturated lipids

Venkatesan, Guru A.; Taylor, Graham J.; Basham, Colin M.; Brady, Nathan G.; Collier, C. Patrick; Sarles, Stephen A.

doi:10.1063/1.5016523

Evaporation-induced monolayer compression improves droplet interface bilayer formation using unsaturated lipids

Journal Article · Wed Feb 28 23:00:00 EST 2018 · Biomicrofluidics

DOI:https://doi.org/10.1063/1.5016523· OSTI ID:1468062

Venkatesan, Guru A. ^[1]; ^[2]; Basham, Colin M. ^[1]; ^[3]; ^[4]; Sarles, Stephen A. ^[1]

Univ. of Tennessee, Knoxville, TN (United States). Department of Mechanical, Aerospace, and Biomedical Engineering
Univ. of Tennessee, Knoxville, TN (United States). Department of Mechanical, Aerospace, and Biomedical Engineering and Bredesen Center; Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Joint Institute for Biological Sciences
Univ. of Tennessee, Knoxville, TN (United States). Department of Biochemistry, Cellular and Molecular Biology
Univ. of Tennessee, Knoxville, TN (United States). Department of Mechanical, Aerospace, and Biomedical Engineering; Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Joint Institute for Biological Sciences and Center for Nanophase Materials Sciences

In this paper, we report on a new experimental methodology to enable reliable formation of droplet interface bilayer (DIB) model membranes with two types of unsaturated lipids that have proven difficult for creating stable DIBs. Through the implementation of a simple evaporation technique to condition the spontaneously assembled lipid monolayer around each droplet, we increased the success rates of DIB formation for two distinct unsaturated lipids, namely 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) and 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC), from less than 10% to near 100%. Separately, using a pendant drop tensiometer, we learned that: (a) DOPC and POPC monolayers do not spontaneously assemble into their tightest possible configurations at an oil-water interface, and (b) reducing the surface area of a water droplet coated with a partially packed monolayer leads to a more tightly packed monolayer with an interfacial tension lower than that achieved by spontaneous assembly alone. We also estimated from Langmuir compression isotherms obtained for both lipids that the brief droplet evaporation procedure prior to DIB formation resulted in a 6%–16% reduction in area per lipid for DOPC and POPC, respectively. Lastly, the increased success rates of formation for DOPC and POPC DIBs enabled quantitative characterization of unsaturated lipid membrane properties including electrical resistance, rupture potential, and specific capacitance.

View Accepted Manuscript (DOE)

Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE Office of Science (SC)

Grant/Contract Number:: AC05-00OR22725

OSTI ID:: 1468062

Journal Information:: Biomicrofluidics, Journal Name: Biomicrofluidics Journal Issue: 2 Vol. 12; ISSN 1932-1058

Publisher:: American Institute of Physics (AIP)Copyright Statement

Country of Publication:: United States

Language:: English

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