Urine and serum biomonitoring of exposure to environmental estrogens II: Soy isoflavones and zearalenone in pregnant women

Fleck, Stefanie C.; Churchwell, Mona I.; Doerge, Daniel R.; Teeguarden, Justin G.

doi:10.1016/j.fct.2016.05.021

Title: Urine and serum biomonitoring of exposure to environmental estrogens II: Soy isoflavones and zearalenone in pregnant women

Journal Article · Mon May 30 00:00:00 EDT 2016 · Food and Chemical Toxicology

DOI:https://doi.org/10.1016/j.fct.2016.05.021· OSTI ID:1988267

Fleck, Stefanie C. ^[1]; Churchwell, Mona I. ^[1]; Doerge, Daniel R. ^[1];

^[2]

U.S. Food and Drug Administration (FDA), Jefferson, AR (United States)
Pacific Northwest National Laboratory (PNNL), Richland, WA (United States); Oregon State Univ., Corvallis, OR (United States)

Urine and serum biomonitoring was used to measure internal exposure to selected dietary estrogens in a cohort of 30 pregnant women. Herein exposure was measured over a period comprising one-half day in the field (6 h) and one day in a clinic (24 h). Biomonitoring of the dietary phytoestrogens genistein (GEN), daidzein (DDZ) and equol (EQ), as well as the mycoestrogen, zearalenone (ZEN) and its congeners, was conducted using UPLC-MS/MS. Biomonitoring revealed evidence of internal exposure to naturally occurring dietary estrogens during pregnancy. Urinary concentrations of total GEN, DDZ and EQ were similar to levels reported for general adult U.S. population. Measurable concentrations of total (parent and metabolites) GEN, DDZ and EQ were present in 240, 207 and 2 of 270 serum samples, respectively. Six out of 30 subjects had measurable concentrations of unconjugated GEN and/or DDZ in serum between 0.6 and 7.1 nM. Urine to serum total isoflavone ratios for GEN, DDZ and EQ were 13, 47, and 180, respectively. ZEN and its reductive metabolite, a-zearalenol (a-ZEL), were present in pregnant women (11 out of 30 subjects) as conjugates at levels near the limit of quantification. The average total urinary concentration was 0.10 mg/L for ZEN and 0.11 mg/L for a-ZEL.

View Accepted Manuscript (DOE)

View Accepted Manuscript (Publisher)

Cite

Export

Save

Research Organization:: Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)

Sponsoring Organization:: USDOE; USDA; American Chemistry Council

Grant/Contract Number:: AC05-76RL01830

OSTI ID:: 1988267

Alternate ID(s):: OSTI ID: 1467737

Report Number(s):: PNNL-ACT-SA-10583

Journal Information:: Food and Chemical Toxicology, Vol. 95; ISSN 0278-6915

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

Citation Metrics:

Cited by: 31 works

Citation information provided by
Web of Science

References (39)

Mass Spectrometric Determination of Genistein Tissue Distribution in Diet-Exposed Sprague-Dawley Rats Churchwell, Mona I.; Delclos, K. Barry; Chang, Hebron C. The Journal of Nutrition, Vol. 130, Issue 8 https://doi.org/10.1093/jn/130.8.1963	journal	August 2000
Preeclampsia and Breast Cancer Risk Innes, Kim E.; Byers, Tim E. Epidemiology, Vol. 10, Issue 6 https://doi.org/10.1097/00001648-199911000-00013	journal	November 1999
Phytoestrogens in Human Pregnancy Jarrell, John; Foster, Warren G.; Kinniburgh, David W. Obstetrics and Gynecology International, Vol. 2012 https://doi.org/10.1155/2012/850313	journal	January 2012
Endocrine-Disrupting Compounds and Mammary Gland Development: Early Exposure and Later Life Consequences Fenton, Suzanne E. Endocrinology, Vol. 147, Issue 6 https://doi.org/10.1210/en.2005-1131	journal	June 2006
Early Intake Appears to Be the Key to the Proposed Protective Effects of Soy Intake Against Breast Cancer Messina, Mark; Hilakivi-Clarke, Leena Nutrition and Cancer, Vol. 61, Issue 6 https://doi.org/10.1080/01635580903285015	journal	November 2009
Zearalenone and its metabolites as endocrine disrupting chemicals Metzler, M.; Pfeiffer, E.; Hildebrand, A. World Mycotoxin Journal, Vol. 3, Issue 4 https://doi.org/10.3920/WMJ2010.1244	journal	November 2010
Toxic Effects of Maternal Zearalenone Exposure on Uterine Capacity and Fetal Development in Gestation Rats Zhang, Yuanyuan; Jia, Zhiqiang; Yin, Shutong Reproductive Sciences, Vol. 21, Issue 6 https://doi.org/10.1177/1933719113512533	journal	June 2014
Urine and serum biomonitoring of exposure to environmental estrogens I: Bisphenol A in pregnant women Teeguarden, Justin G.; Twaddle, Nathan C.; Churchwell, Mona I. Food and Chemical Toxicology, Vol. 92 https://doi.org/10.1016/j.fct.2016.03.023	journal	June 2016
Equol, a natural estrogenic metabolite from soy isoflavones Muthyala, Rajeev S.; Ju, Young H.; Sheng, Shubin Bioorganic & Medicinal Chemistry, Vol. 12, Issue 6 https://doi.org/10.1016/j.bmc.2003.11.035	journal	March 2004
Isoflavones: estrogenic activity, biological effect and bioavailability Vitale, Daniela Cristina; Piazza, Cateno; Melilli, Barbara European Journal of Drug Metabolism and Pharmacokinetics, Vol. 38, Issue 1 https://doi.org/10.1007/s13318-012-0112-y	journal	November 2012
Early Telarche Symptoms in Children and their Relations to Zearalenon Contamination in Foodstuffs Szuets, P.; Mesterhazy, A.; Falkay, G. Y. Cereal Research Communications, Vol. 25, Issue 3 https://doi.org/10.1007/BF03543747	journal	September 1997
Competitive Binding of Xenobiotic Oestrogens to Rat Alpha-Fetoprotein and to Sex Steroid Binding Proteins in Human and Rainbow Trout (Oncorhynchus mykiss) Plasma Milligan, Stuart R.; Khan, Omar; Nash, Marnie General and Comparative Endocrinology, Vol. 112, Issue 1 https://doi.org/10.1006/gcen.1998.7146	journal	October 1998
Phytoestrogen Concentrations in Serum from Japanese Men and Women over Forty Years of Age Morton, Michael S.; Arisaka, Osamu; Miyake, Noriko The Journal of Nutrition, Vol. 132, Issue 10 https://doi.org/10.1093/jn/131.10.3168	journal	October 2002
Equol: History, Chemistry, and Formation Setchell, Kenneth D. R.; Clerici, Carlo The Journal of Nutrition, Vol. 140, Issue 7 https://doi.org/10.3945/jn.109.119776	journal	July 2010
Placental transfer of the estrogenic mycotoxin zearalenone in rats Bernhoft, Aksel; Behrens, Gerd H. G.; Ingebrigtsen, Kristian Reproductive Toxicology, Vol. 15, Issue 5 https://doi.org/10.1016/S0890-6238(01)00159-9	journal	September 2001
Urinary and serum concentrations of seven phytoestrogens in a human reference population subset Valentín-Blasini, Liza; Blount, Benjamin C.; Caudill, Samuel P. Journal of Exposure Science & Environmental Epidemiology, Vol. 13, Issue 4 https://doi.org/10.1038/sj.jea.7500278	journal	July 2003
Isolation of an Anabolic, Uterotrophic Compound from Corn infected with Gibberella zeae Stob, Martin; Baldwin, R. S.; Tuite, John Nature, Vol. 196, Issue 4861 https://doi.org/10.1038/1961318a0	journal	December 1962
Transgenerational toxicity of Zearalenone in pigs Schoevers, Eric J.; Santos, Regiane R.; Colenbrander, Ben Reproductive Toxicology, Vol. 34, Issue 1 https://doi.org/10.1016/j.reprotox.2012.03.004	journal	August 2012
Comparative metabolism of zearalenone and transmission into bovine milk Mirocha, C. J.; Pathre, S. V.; Robison, T. S. Food and Cosmetics Toxicology, Vol. 19 https://doi.org/10.1016/0015-6264(81)90299-6	journal	January 1981
Premature Thelarche in Puerto Rico Freni-Titulaer, Lambertina W. American Journal of Diseases of Children, Vol. 140, Issue 12 https://doi.org/10.1001/archpedi.1986.02140260065028	journal	December 1986
In Utero Exposures and Breast Cancer: a Study of Opposite-Sexed Twins Kaijser, M.; Lichtenstein, P.; Granath, F. JNCI Journal of the National Cancer Institute, Vol. 93, Issue 1 https://doi.org/10.1093/jnci/93.1.60	journal	January 2001
Reproductive consequences of developmental phytoestrogen exposure Jefferson, Wendy N.; Patisaul, Heather B.; Williams, Carmen J. REPRODUCTION, Vol. 143, Issue 3 https://doi.org/10.1530/REP-11-0369	journal	March 2012
Maternal and neonatal phytoestrogens in Japanese women during birth Adlercreutz, Herman; Yamada, Teiichi; Wähälä, Kristiina American Journal of Obstetrics and Gynecology, Vol. 180, Issue 3 https://doi.org/10.1016/S0002-9378(99)70281-4	journal	March 1999
Effects of dietary daidzein and its metabolite, equol, at physiological concentrations on the growth of estrogen-dependent human breast cancer (MCF-7) tumors implanted in ovariectomized athymic mice Ju, Young H.; Fultz, Jodi; Allred, Kimberly F. Carcinogenesis, Vol. 27, Issue 4 https://doi.org/10.1093/carcin/bgi320	journal	January 2006
Rapid yeast estrogen bioassays stably expressing human estrogen receptors α and β, and green fluorescent protein: a comparison of different compounds with both receptor types Bovee, Toine F. H.; Helsdingen, Richard J. R.; Rietjens, Ivonne M. C. M. The Journal of Steroid Biochemistry and Molecular Biology, Vol. 91, Issue 3 https://doi.org/10.1016/j.jsbmb.2004.03.118	journal	July 2004
An epidemic of precocious development in Puerto Rican children Sáenz de Rodriguez, Carmen A.; Bongiovanni, Alfred M.; Borrego, Lillian Conde de The Journal of Pediatrics, Vol. 107, Issue 3 https://doi.org/10.1016/S0022-3476(85)80513-8	journal	September 1985
High Growth Rate of Girls with Precocious Puberty Exposed to Estrogenic Mycotoxins Massart, Francesco; Meucci, Valentina; Saggese, Giuseppe The Journal of Pediatrics, Vol. 152, Issue 5 https://doi.org/10.1016/j.jpeds.2007.10.020	journal	May 2008
Assessment of Multi-Mycotoxin Exposure in Southern Italy by Urinary Multi-Biomarker Determination Solfrizzo, Michele; Gambacorta, Lucia; Visconti, Angelo Toxins, Vol. 6, Issue 2 https://doi.org/10.3390/toxins6020523	journal	January 2014
Comparing the pharmacokinetics of daidzein and genistein with the use of 13C-labeled tracers in premenopausal women Setchell, Kenneth DR; Faughnan, Marian S.; Avades, Tony The American Journal of Clinical Nutrition, Vol. 77, Issue 2 https://doi.org/10.1093/ajcn/77.2.411	journal	February 2003
Quantitative assessment of foetal exposure to trenbolone acetate, zeranol and melengestrol acetate, following maternal dosing in rabbits Lange, I. G.; Daxenberger, A.; Meyer, H. H. D. Xenobiotica, Vol. 32, Issue 8 https://doi.org/10.1080/00498250210143010	journal	January 2002
Bioavailability of phyto-oestrogens Rowland, Ian; Faughnan, Marian; Hoey, Leane British Journal of Nutrition, Vol. 89, Issue S1 https://doi.org/10.1079/BJN2002796	journal	June 2003
Biomonitoring of concurrent mycotoxin exposure among adults in Sweden through urinary multi-biomarker analysis Wallin, S.; Gambacorta, L.; Kotova, N. Food and Chemical Toxicology, Vol. 83 https://doi.org/10.1016/j.fct.2015.05.023	journal	September 2015
Safety and pharmacokinetics of purified soy isoflavones: single-dose administration to postmenopausal women,, Bloedon, LeAnne T.; Jeffcoat, A. Robert; Lopaczynski, Wlodek The American Journal of Clinical Nutrition, Vol. 76, Issue 5 https://doi.org/10.1093/ajcn/76.5.1126	journal	November 2002
Exposures to Synthetic Estrogens at Different Times During the Life, and Their Effect on Breast Cancer Risk Hilakivi-Clarke, Leena; de Assis, Sonia; Warri, Anni Journal of Mammary Gland Biology and Neoplasia, Vol. 18, Issue 1 https://doi.org/10.1007/s10911-013-9274-8	journal	February 2013
New insights into the human metabolism of the Fusarium mycotoxins deoxynivalenol and zearalenone Warth, Benedikt; Sulyok, Michael; Berthiller, Franz Toxicology Letters, Vol. 220, Issue 1 https://doi.org/10.1016/j.toxlet.2013.04.012	journal	June 2013
Comparison of Isoflavones Among Dietary Intake, Plasma Concentration and Urinary Excretion for Accurate Estimation of Phytoestrogen Intake Arai, Yusuke; Uehara, Mariko; Sato, Yuho Journal of Epidemiology, Vol. 10, Issue 2 https://doi.org/10.2188/jea.10.127	journal	January 2000
Physiological Concentrations of Dietary Genistein Dose-Dependently Stimulate Growth of Estrogen-Dependent Human Breast Cancer (MCF-7) Tumors Implanted in Athymic Nude Mice Ju, Young H.; Allred, Clinton D.; Allred, Kimberly F. The Journal of Nutrition, Vol. 131, Issue 11 https://doi.org/10.1093/jn/131.11.2957	journal	November 2001
Plasma Levels of Unconjugated Estrone, Estradiol and Estriol and of HCS Throughout Pregnancy in Normal Women De Hertogh, R.; Thomas, K.; Bietlot, Y. The Journal of Clinical Endocrinology & Metabolism, Vol. 40, Issue 1 https://doi.org/10.1210/jcem-40-1-93	journal	January 1975
Placental transfer of the soy isoflavone genistein following dietary and gavage administration to Sprague Dawley rats Doerge, Daniel R.; Churchwell, Mona I.; Chang, Hebron C. Reproductive Toxicology, Vol. 15, Issue 2 https://doi.org/10.1016/S0890-6238(01)00108-3	journal	March 2001

Cited By (2)

Towards A New Approach for the Description of Cyclo–2,4-Dihydroxybenzoate, A Substance Which Effectively Mimics Zearalenone in Imprinted Polymers Designed for Analyzing Selected Mycotoxins in Urine Gadzała-Kopciuch, Renata; Kwaśniewska, Katarzyna; Ludwiczak, Agnieszka International Journal of Molecular Sciences, Vol. 20, Issue 7 https://doi.org/10.3390/ijms20071588	journal	March 2019
Oestrogenic Endocrine Disruptors in the Placenta and the Fetus Tang, Zi-Run; Xu, Xue-Ling; Deng, Shou-Long International Journal of Molecular Sciences, Vol. 21, Issue 4 https://doi.org/10.3390/ijms21041519	journal	February 2020

Similar Records

Comparative estrogenicity of endogenous, environmental and dietary estrogens in pregnant women I: Serum levels, variability and the basis for urinary biomonitoring of serum estrogenicity

Journal Article · Tue May 01 00:00:00 EDT 2018 · Food and Chemical Toxicology · OSTI ID:1988267

Fleck, Stefanie C.; Twaddle, Nathan C.; Churchwell, Mona I.; +3 more

Comparative estrogenicity of endogenous, environmental and dietary estrogens in pregnant women II: Total estrogenicity calculations accounting for competitive protein and receptor binding and potency

Journal Article · Thu Dec 13 00:00:00 EST 2018 · Food and Chemical Toxicology · OSTI ID:1988267

Pande, Paritosh; Fleck, Stefanie C.; Twaddle, Nathan C.; +3 more

Characterization and therapeutic alteration of the biliary excretion and enterohepatic cycling of zearalenone in sexually immature swine

Miscellaneous · Sun Jan 01 00:00:00 EST 1989 · OSTI ID:1988267

Biehl, M L

Related Subjects

60 APPLIED LIFE SCIENCES
soy isoflavones
zearalenone
exposure
pregnancy
biomonitoring
endocrine disruptors

Title: Urine and serum biomonitoring of exposure to environmental estrogens II: Soy isoflavones and zearalenone in pregnant women

Citation Formats

References (39)

Cited By (2)

Similar Records

Related Subjects