Structure of the Fc fragment of the NIST reference antibody RM8671

Gallagher, D. Travis; Galvin, Connor V.; Karageorgos, Ioannis

doi:10.1107/S2053230X18009834

Structure of the Fc fragment of the NIST reference antibody RM8671

Journal Article · Wed Aug 29 00:00:00 EDT 2018 · Acta Crystallographica. Section F, Structural Biology Communications

DOI:https://doi.org/10.1107/S2053230X18009834· OSTI ID:1467531

Gallagher, D. Travis; Galvin, Connor V.; Karageorgos, Ioannis

As the link between antigen binding and immune activation, the antibody Fc region has received extensive structural study. In this report, the structure of the Fc fragment of the NIST IgG1 mAb (reference material 8671) is described at 2.1 Å resolution in space group P 2 ₁ 2 ₁ 2 ₁ , with approximate unit-cell parameters a = 50, b = 80, c = 138 Å. Prior Fc structures with a wide variety of modifications are also surveyed, focusing on those in the same crystal form. To facilitate the analysis of conformations, a reference frame and a two-parameter metric are proposed, considering the CH2 domains as mobile with respect to a fixed dimeric CH3 core. Over several human Fc structures, a significant variation in Fc elbow conformations is observed, which may serve to facilitate the regulation of Fc effector signaling.

Sponsoring Organization:: USDOE

Grant/Contract Number:: AC02-06CH11357

OSTI ID:: 1467531

Alternate ID(s):: OSTI ID: 1511726

Journal Information:: Acta Crystallographica. Section F, Structural Biology Communications, Journal Name: Acta Crystallographica. Section F, Structural Biology Communications Journal Issue: 9 Vol. 74; ISSN ACSFEN; ISSN 2053-230X

Publisher:: International Union of Crystallography (IUCr)Copyright Statement

Country of Publication:: United Kingdom

Language:: English

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