Blumenols as shoot markers of root symbiosis with arbuscular mycorrhizal fungi

Wang, Ming; Schäfer, Martin; Li, Dapeng; Halitschke, Rayko; Dong, Chuanfu; McGale, Erica; Paetz, Christian; Song, Yuanyuan; Li, Suhua; Dong, Junfu; Heiling, Sven; Groten, Karin; Franken, Philipp; Bitterlich, Michael; Harrison, Maria J.; Paszkowski, Uta; Baldwin, Ian T.

doi:10.7554/eLife.37093

Blumenols as shoot markers of root symbiosis with arbuscular mycorrhizal fungi

Journal Article · Tue Aug 28 00:00:00 EDT 2018 · eLife

DOI:https://doi.org/10.7554/eLife.37093· OSTI ID:1467130

Wang, Ming ^[1]; ^[1]; Li, Dapeng ^[1]; ^[1]; ^[2]; ^[1]; Paetz, Christian ^[3]; Song, Yuanyuan ^[1]; Li, Suhua ^[1]; Dong, Junfu ^[4]; Heiling, Sven ^[1]; Groten, Karin ^[1]; ^[5]; ^[6]; ^[7]; ^[8]; ^[1]

Department of Molecular Ecology, Max Planck Institute for Chemical Ecology, Jena, Germany
Department of Bioorganic Chemistry, Max Planck Institute for Chemical Ecology, Jena, Germany
Research Group Biosynthesis / NMR, Max Planck Institute for Chemical Ecology, Jena, Germany
Department of Molecular Ecology, Max Planck Institute for Chemical Ecology, Jena, Germany, College of Life Sciences, University of Chinese Academy of Sciences, Beijing, China
Leibniz-Institute of Vegetable and Ornamental Crops, Grossbeeren, Germany, Institute of Biology, Humboldt Universität zu Berlin, Berlin, Germany
Leibniz-Institute of Vegetable and Ornamental Crops, Grossbeeren, Germany
Boyce Thompson Institute for Plant Research, Ithaca, United States
Department of Plant Sciences, University of Cambridge, Cambridge, United Kingdom

High-through-put (HTP) screening for functional arbuscular mycorrhizal fungi (AMF)-associations is challenging because roots must be excavated and colonization evaluated by transcript analysis or microscopy. Here we show that specific leaf-metabolites provide broadly applicable accurate proxies of these associations, suitable for HTP-screens. With a combination of untargeted and targeted metabolomics, we show that shoot accumulations of hydroxy- and carboxyblumenol C-glucosides mirror root AMF-colonization in Nicotiana attenuata plants. Genetic/pharmacologic manipulations indicate that these AMF-indicative foliar blumenols are synthesized and transported from roots to shoots. These blumenol-derived foliar markers, found in many di- and monocotyledonous crop and model plants (Solanum lycopersicum, Solanum tuberosum, Hordeum vulgare, Triticum aestivum, Medicago truncatula and Brachypodium distachyon), are not restricted to particular plant-AMF interactions, and are shown to be applicable for field-based QTL mapping of AMF-related genes.

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Sponsoring Organization:: USDOE

Grant/Contract Number:: SC0012460

OSTI ID:: 1467130

Journal Information:: eLife, Journal Name: eLife Vol. 7; ISSN 2050-084X

Publisher:: eLife Sciences Publications, Ltd.Copyright Statement

Country of Publication:: United States

Language:: English

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Blumenols as shoot markers of root symbiosis with arbuscular mycorrhizal fungi

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Blumenols as shoot markers of root symbiosis with arbuscular mycorrhizal fungi

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