Plant‐made E2 glycoprotein single‐dose vaccine protects pigs against classical swine fever
- Department of Biological and Health Sciences Texas A&,M University Kingsville Kingsville TX USA
- Department of Anatomy and Physiology Kansas State University Manhattan KS USA
- iBio CDMO Bryan TX USA
- U.S. Department of Homeland Security Science and Technology Directorate Plum Island Animal Disease Center Greenport New York USA
- Oak Ridge Institute for Science and Education Plum Island Animal Disease Center Research Participation Program Oak Ridge TN USA
- BioQuest Associates LLC Plum Island Animal Disease Center Greenport New York USA
- Department of Veterinary Pathobiology Texas A&,M University College Station TX USA
Abstract Classical Swine Fever Virus ( CSFV ) causes classical swine fever, a highly contagious hemorrhagic fever affecting both feral and domesticated pigs. Outbreaks of CSF in Europe, Asia, Africa and South America had significant adverse impacts on animal health, food security and the pig industry. The disease is generally contained by prevention of exposure through import restrictions ( e.g . banning import of live pigs and pork products), localized vaccination programmes and culling of infected or at‐risk animals, often at very high cost. Current CSFV ‐modified live virus vaccines are protective, but do not allow differentiation of infected from vaccinated animals ( DIVA ), a critical aspect of disease surveillance programmes. Alternatively, first‐generation subunit vaccines using the viral protein E2 allow for use of DIVA diagnostic tests, but are slow to induce a protective response, provide limited prevention of vertical transmission and may fail to block viral shedding. CSFV E2 subunit vaccines from a baculovirus/insect cell system have been developed for several vaccination campaigns in Europe and Asia. However, this expression system is considered expensive for a veterinary vaccine and is not ideal for wide‐spread deployment. To address the issues of scalability, cost of production and immunogenicity, we have employed an Agrobacterium ‐mediated transient expression platform in Nicotiana benthamiana and formulated the purified antigen in novel oil‐in‐water emulsion adjuvants. We report the manufacturing of adjuvanted, plant‐made CSFV E2 subunit vaccine. The vaccine provided complete protection in challenged pigs, even after single‐dose vaccination, which was accompanied by strong virus neutralization antibody responses.
- Research Organization:
- Oak Ridge Institute for Science and Education (ORISE), Oak Ridge, TN (United States)
- Sponsoring Organization:
- USDOE
- Grant/Contract Number:
- 59-5430-001-23S, NP-103; 2010-ST-061-AG0002; GS-23F-8006H; AC05‐06OR23100
- OSTI ID:
- 1463922
- Alternate ID(s):
- OSTI ID: 1463924; OSTI ID: 1580727
- Journal Information:
- Plant Biotechnology Journal, Journal Name: Plant Biotechnology Journal Vol. 17 Journal Issue: 2; ISSN 1467-7644
- Publisher:
- Wiley-BlackwellCopyright Statement
- Country of Publication:
- United Kingdom
- Language:
- English
Web of Science
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