Mitochondrial protein functions elucidated by multi-omic mass spectrometry profiling

Stefely, Jonathan A.; Kwiecien, Nicholas W.; Freiberger, Elyse C.; Richards, Alicia L.; Jochem, Adam; Rush, Matthew J. P.; Ulbrich, Arne; Robinson, Kyle P.; Hutchins, Paul D.; Veling, Mike T.; Guo, Xiao; Kemmerer, Zachary A.; Connors, Kyle J.; Trujillo, Edna A.; Sokol, Jacob; Marx, Harald; Westphall, Michael S.; Hebert, Alexander S.; Pagliarini, David J.; Coon, Joshua J.

doi:10.1038/nbt.3683

Mitochondrial protein functions elucidated by multi-omic mass spectrometry profiling

Journal Article · Mon Sep 26 00:00:00 UTC 2016 · Nature Biotechnology

DOI:https://doi.org/10.1038/nbt.3683· OSTI ID:1463133

Stefely, Jonathan A. ^[1]; Kwiecien, Nicholas W. ^[2]; Freiberger, Elyse C. ^[2]; Richards, Alicia L. ^[2]; Jochem, Adam ^[3]; Rush, Matthew J. P. ^[2]; Ulbrich, Arne ^[2]; Robinson, Kyle P. ^[4]; Hutchins, Paul D. ^[2]; Veling, Mike T. ^[4]; Guo, Xiao ^[4]; Kemmerer, Zachary A. ^[4]; Connors, Kyle J. ^[2]; Trujillo, Edna A. ^[2]; Sokol, Jacob ^[4]; Marx, Harald ^[5]; Westphall, Michael S. ^[5]; Hebert, Alexander S. ^[5]; Pagliarini, David J. ^[4]; Coon, Joshua J. ^[2]

Univ. of Wisconsin, Madison, WI (United States); Morgridge Inst. for Research, Madison, WI (United States); OSTI
Univ. of Wisconsin, Madison, WI (United States); Genome Center of Wisconsin, Madison, WI (United States)
Morgridge Inst. for Research, Madison, WI (United States)
Univ. of Wisconsin, Madison, WI (United States); Morgridge Inst. for Research, Madison, WI (United States)
Genome Center of Wisconsin, Madison, WI (United States)

Mitochondrial dysfunction is associated with many human diseases, including cancer and neurodegeneration, that are often linked to proteins and pathways that are not well-characterized. To begin defining the functions of such poorly characterized proteins, we used mass spectrometry to map the proteomes, lipidomes and metabolomes of 174 yeast strains, each lacking a single gene related to mitochondrial biology. 144 of these genes have human homologs, 60 of which are associated with disease and 39 of which are uncharacterized. We present a multi-omic data analysis and visualization tool that we use to find covariance networks that can predict molecular functions, correlations between profiles of related gene deletions, gene-specific perturbations that reflect protein functions, and a global respiration deficiency response. Using this multi-omic approach, we link seven proteins including Hfd1p and its human homolog ALDH3A1 to mitochondrial coenzyme Q (CoQ) biosynthesis, an essential pathway disrupted in many human diseases. This Resource should provide broad molecular insights into mitochondrial protein functions.

View Accepted Manuscript (DOE)

Research Organization:: Univ. of Wisconsin, Madison, WI (United States)

Sponsoring Organization:: USDOE

Grant/Contract Number:: FC02-07ER64494

OSTI ID:: 1463133

Journal Information:: Nature Biotechnology, Journal Name: Nature Biotechnology Journal Issue: 11 Vol. 34; ISSN 1087-0156

Publisher:: Springer NatureCopyright Statement

Country of Publication:: United States

Language:: English

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