Protein hyperproduction in fungi by design

Baker, Scott E.

doi:10.1007/s00253-018-9265-1

Title: Protein hyperproduction in fungi by design

Journal Article · Sat Aug 04 00:00:00 EDT 2018 · Applied Microbiology and Biotechnology

DOI:https://doi.org/10.1007/s00253-018-9265-1· OSTI ID:1463032

The secretion of enzymes used by fungi to digest their environment has been exploited by humans for centuries for food and beverage production. More than a century after the first biotechnology patent, we know that the enzyme cocktails secreted by these amazing organisms have tremendous use across a number of industrial processes. Secreting the maximum titer of enzymes is critical to the economic feasibility of these processes. Traditional mutagenesis and screening approaches have generated the vast majority of strains used by industry for the production of enzymes. Until the emergence of economical next generation DNA sequencing platforms, the majority of the genes mutated in these screens remained uncharacterized at the sequence level. In addition, mutagenesis comes with a cost to an organism’s fitness, making tractable rational strain design approaches an attractive alternative. As an alternative to traditional utagenesis and screening, controlled manipulation of multiple genes involved in processes that impact the ability of a fungus to sense its environment, regulate transcription of enzyme-encoding genes, and efficiently secrete these proteins will allow for rational design of improved fungal protein production strains.

View Journal Article

Cite

Export

Save

Research Organization:: Pacific Northwest National Laboratory (PNNL), Richland, WA (United States); Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: AC05-76RL01830; AC02-05CH11231; AC0576RL01830

OSTI ID:: 1463032

Alternate ID(s):: OSTI ID: 1468613; OSTI ID: 1513534

Report Number(s):: PNNL-SA-137094; PII: 9265

Journal Information:: Applied Microbiology and Biotechnology, Journal Name: Applied Microbiology and Biotechnology; ISSN 0175-7598

Publisher:: SpringerCopyright Statement

Country of Publication:: Germany

Language:: English

Citation Metrics:

Cited by: 8 works

Citation information provided by
Web of Science

References (96)

Enhanced Cellulase Production by a Mutant of Trichoderma viride Mandels, Mary; Weber, James; Parizek, Richard Applied Microbiology, Vol. 21, Issue 1 https://doi.org/10.1128/AEM.21.1.152-154.1971	journal	January 1971
Global Carbon Utilization Profiles of Wild-Type, Mutant, and Transformant Strains of Hypocrea jecorina Druzhinina, I. S.; Schmoll, M.; Seiboth, B. Applied and Environmental Microbiology, Vol. 72, Issue 3 https://doi.org/10.1128/AEM.72.3.2126-2133.2006	journal	March 2006
Cell wall synthesis and central carbohydrate metabolism are interconnected by the SNF1/Mig1 pathway in Kluyveromyces lactis Rippert, Dorthe; Backhaus, Katja; Rodicio, Rosaura European Journal of Cell Biology, Vol. 96, Issue 1 https://doi.org/10.1016/j.ejcb.2016.12.004	journal	January 2017
On the safety of Aspergillus niger - a review Schuster, E.; Dunn-Coleman, N.; Frisvad, J. Applied Microbiology and Biotechnology, Vol. 59, Issue 4-5, p. 426-435 https://doi.org/10.1007/s00253-002-1032-6	journal	January 2002
Regions in the promoter of the yeast FBP1 gene implicated in catabolite repression may bind the product of the regulatory gene MIG1 Mercado, Juan J.; Vincent, Olivier; Gancedo, Juana M. FEBS Letters, Vol. 291, Issue 1 https://doi.org/10.1016/0014-5793(91)81112-L	journal	October 1991
The unfolded protein response: the dawn of a new field Mori, Kazutoshi Proceedings of the Japan Academy, Series B, Vol. 91, Issue 9 https://doi.org/10.2183/pjab.91.469	journal	January 2015
Carbon Regulation of Ribosomal Genes in Neurospora crassa Occurs by a Mechanism Which Does Not Require Cre-1, the Homologue of the Aspergillus Carbon Catabolite Repressor, CreA de la Serna, Ivana; Ng, Dean; Tyler, Brett M. Fungal Genetics and Biology, Vol. 26, Issue 3 https://doi.org/10.1006/fgbi.1999.1121	journal	April 1999
Mating type genes and cryptic sexuality as tools for genetically manipulating industrial molds Kück, Ulrich; Böhm, Julia Applied Microbiology and Biotechnology, Vol. 97, Issue 22 https://doi.org/10.1007/s00253-013-5268-0	journal	October 2013
Genomics of Aspergillus oryzae: Learning from the History of Koji Mold and Exploration of Its Future Machida, M.; Yamada, O.; Gomi, K. DNA Research, Vol. 15, Issue 4 https://doi.org/10.1093/dnares/dsn020	journal	May 2008
Nitrogen metabolite repression in Aspergillus nidulans Arst, Herbert N.; Cove, David J. MGG Molecular & General Genetics, Vol. 126, Issue 2 https://doi.org/10.1007/BF00330988	journal	January 1973
Involvement of the adaptor protein 3 complex in lignocellulase secretion in Neurospora crassa revealed by comparative genomic screening Pei, Xue; Fan, Feiyu; Lin, Liangcai Biotechnology for Biofuels, Vol. 8, Issue 1 https://doi.org/10.1186/s13068-015-0302-3	journal	August 2015
Synergistic and Dose-Controlled Regulation of Cellulase Gene Expression in Penicillium oxalicum Li, Zhonghai; Yao, Guangshan; Wu, Ruimei PLOS Genetics, Vol. 11, Issue 9 https://doi.org/10.1371/journal.pgen.1005509	journal	September 2015
Identification and characterization of the glucose dual-affinity transport system in Neurospora crassa: pleiotropic roles in nutrient transport, signaling, and carbon catabolite repression Wang, Bang; Li, Jingen; Gao, Jingfang Biotechnology for Biofuels, Vol. 10, Issue 1 https://doi.org/10.1186/s13068-017-0705-4	journal	January 2017
Functional and Genomic Analyses Reveal an Essential Coordination between the Unfolded Protein Response and ER-Associated Degradation Travers, Kevin J.; Patil, Christopher K.; Wodicka, Lisa Cell, Vol. 101, Issue 3 https://doi.org/10.1016/S0092-8674(00)80835-1	journal	April 2000
Direct involvement of the CreA transcription factor in penicillin biosynthesis and expression of the pcbAB gene in Penicillium chrysogenum Cepeda-García, Cristina; Domínguez-Santos, Rebeca; García-Rico, Ramón O. Applied Microbiology and Biotechnology, Vol. 98, Issue 16 https://doi.org/10.1007/s00253-014-5760-1	journal	May 2014
Control of yeast GAL genes by MIG1 repressor: a transcriptional cascade in the glucose response. Nehlin, J. O.; Carlberg, M.; Ronne, H. The EMBO Journal, Vol. 10, Issue 11 https://doi.org/10.1002/j.1460-2075.1991.tb04901.x	journal	November 1991
Improvement of Foreign-Protein Production in Aspergillus niger var. awamori by Constitutive Induction of the Unfolded-Protein Response Valkonen, M.; Ward, M.; Wang, H. Applied and Environmental Microbiology, Vol. 69, Issue 12 https://doi.org/10.1128/AEM.69.12.6979-6986.2003	journal	December 2003
The HAC1 gene from Pichia pastoris: characterization and effect of its overexpression on the production of secreted, surface displayed and membrane proteins Guerfal, Mouna; Ryckaert, Stefan; Jacobs, Pieter P. Microbial Cell Factories, Vol. 9, Issue 1 https://doi.org/10.1186/1475-2859-9-49	journal	January 2010
Genome-wide expression analysis upon constitutive activation of the HacA bZIP transcription factor in Aspergillus niger reveals a coordinated cellular response to counteract ER stress Carvalho, Neuza DSP; Jørgensen, Thomas R.; Arentshorst, Mark BMC Genomics, Vol. 13, Issue 1 https://doi.org/10.1186/1471-2164-13-350	journal	January 2012
Carbon Catabolite Repression of Gene Expression and Conidiation inNeurospora crassa Ebbole, Daniel J. Fungal Genetics and Biology, Vol. 25, Issue 1 https://doi.org/10.1006/fgbi.1998.1088	journal	October 1998
Activation mechanisms of the HACI-mediated unfolded protein response in filamentous fungi: M. Saloheimo, M. Valkonen and M. Penttillä Saloheimo, Markku; Valkonen, Mari; Penttilä, Merja Molecular Microbiology, Vol. 47, Issue 4 https://doi.org/10.1046/j.1365-2958.2003.03363.x	journal	February 2003
Array comparative genomic hybridization analysis of Trichoderma reesei strains with enhanced cellulase production properties Vitikainen, Marika; Arvas, Mikko; Pakula, Tiina BMC Genomics, Vol. 11, Issue 1 https://doi.org/10.1186/1471-2164-11-441	journal	January 2010
Activation of the Transducers of Unfolded Protein Response in Plants Nawkar, Ganesh M.; Lee, Eun Seon; Shelake, Rahul M. Frontiers in Plant Science, Vol. 9 https://doi.org/10.3389/fpls.2018.00214	journal	February 2018
Nutrient Sensing at the Plasma Membrane of Fungal Cells Van Dijck, Patrick; Brown, Neil Andrew; Goldman, Gustavo H. Microbiology Spectrum, Vol. 5, Issue 2 https://doi.org/10.1128/microbiolspec.FUNK-0031-2016	journal	April 2017
Redesigning the regulatory pathway to enhance cellulase production in Penicillium oxalicum Yao, Guangshan; Li, Zhonghai; Gao, Liwei Biotechnology for Biofuels, Vol. 8, Issue 1 https://doi.org/10.1186/s13068-015-0253-8	journal	April 2015
Lipid-dependent regulation of the unfolded protein response Volmer, Romain; Ron, David Current Opinion in Cell Biology, Vol. 33 https://doi.org/10.1016/j.ceb.2014.12.002	journal	April 2015
G-protein coupled receptor-mediated nutrient sensing and developmental control in A spergillus nidulans : G-protein coupled receptor-mediated nutrient sensing and developmental control Brown, Neil Andrew; dos Reis, Thaila Fernanda; Ries, Laure Nicolas Annick Molecular Microbiology, Vol. 98, Issue 3 https://doi.org/10.1111/mmi.13135	journal	August 2015
Changes in transcript levels of starch hydrolysis genes and raising citric acid production via carbon ion irradiation mutagenesis of Aspergillus niger Hu, Wei; Li, Wenjian; Chen, Hao PLOS ONE, Vol. 12, Issue 6 https://doi.org/10.1371/journal.pone.0180120	journal	June 2017
Signalling from endoplasmic reticulum to nucleus: transcription factor with a basic-leucine zipper motif is required for the unfolded protein-response pathway Mori, Kazutoshi; Kawahara, Tetsushi; Yoshida, Hiderou Genes to Cells, Vol. 1, Issue 9 https://doi.org/10.1046/j.1365-2443.1996.d01-274.x	journal	September 1996
Roles of Protein Kinase A and Adenylate Cyclase in Light-Modulated Cellulase Regulation in Trichoderma reesei Schuster, André; Tisch, Doris; Seidl-Seiboth, Verena Applied and Environmental Microbiology, Vol. 78, Issue 7 https://doi.org/10.1128/AEM.06959-11	journal	January 2012
Proximity ligation scaffolding and comparison of two Trichoderma reesei strains genomes Jourdier, Etienne; Baudry, Lyam; Poggi-Parodi, Dante Biotechnology for Biofuels, Vol. 10, Issue 1 https://doi.org/10.1186/s13068-017-0837-6	journal	June 2017
GPR-4 Is a Predicted G-Protein-Coupled Receptor Required for Carbon Source-Dependent Asexual Growth and Development in Neurospora crassa Li, Liande; Borkovich, Katherine A. Eukaryotic Cell, Vol. 5, Issue 8 https://doi.org/10.1128/EC.00109-06	journal	August 2006
Cloning of the creA gene from Aspergillus nidulans: a gene involved in carbon catabolite repression Dowzer, Celia E. A.; Kelly, Joan M. Current Genetics, Vol. 15, Issue 6 https://doi.org/10.1007/BF00376804	journal	June 1989
The importance of connections between the cell wall integrity pathway and the unfolded protein response in filamentous fungi Malavazi, I.; Goldman, G. H.; Brown, N. A. Briefings in Functional Genomics, Vol. 13, Issue 6 https://doi.org/10.1093/bfgp/elu027	journal	July 2014
Effects of Inactivation and Constitutive Expression of the Unfolded- Protein Response Pathway on Protein Production in the Yeast Saccharomyces cerevisiae Valkonen, M.; Penttila, M.; Saloheimo, M. Applied and Environmental Microbiology, Vol. 69, Issue 4 https://doi.org/10.1128/AEM.69.4.2065-2072.2003	journal	April 2003
Preparation of mutants of Trichoderma reesei with enhanced cellulase production. Montenecourt, B. S.; Eveleigh, D. E. Applied and Environmental Microbiology, Vol. 34, Issue 6 https://doi.org/10.1128/AEM.34.6.777-782.1977	journal	January 1977
Trichoderma reesei RUT-C30 - thirty years of strain improvement Peterson, R.; Nevalainen, H. Microbiology, Vol. 158, Issue 1, p. 58-68 https://doi.org/10.1099/mic.0.054031-0	journal	October 2011
The Effects of Drugs Inhibiting Protein Secretion in the Filamentous Fungus Trichoderma reesei : EVIDENCE FOR DOWN-REGULATION OF GENES THAT ENCODE SECRETED PROTEINS IN THE STRESSED CELLS Pakula, Tiina M.; Laxell, Marjukka; Huuskonen, Anne Journal of Biological Chemistry, Vol. 278, Issue 45 https://doi.org/10.1074/jbc.M302372200	journal	August 2003
Yeast MIG1 repressor is related to the mammalian early growth response and Wilms' tumour finger proteins. Nehlin, J. O.; Ronne, H. The EMBO Journal, Vol. 9, Issue 9 https://doi.org/10.1002/j.1460-2075.1990.tb07479.x	journal	September 1990
Trichoderma reesei : genetic approaches to improving strain efficiency Seidl, Verena; Seiboth, Bernhard Biofuels, Vol. 1, Issue 2 https://doi.org/10.4155/bfs.10.1	journal	March 2010
Tracking the roots of cellulase hyperproduction by the fungus Trichoderma reesei using massively parallel DNA sequencing Le Crom, S.; Schackwitz, W.; Pennacchio, L. Proceedings of the National Academy of Sciences, Vol. 106, Issue 38 https://doi.org/10.1073/pnas.0905848106	journal	September 2009
Regulators of plant biomass degradation in ascomycetous fungi Benocci, Tiziano; Aguilar-Pontes, Maria Victoria; Zhou, Miaomiao Biotechnology for Biofuels, Vol. 10, Issue 1 https://doi.org/10.1186/s13068-017-0841-x	journal	June 2017
Loss of glucose repression in an Acremonium chrysogenum β-lactam producer strain and its restoration by multiple copies of the cre1 gene Jekosch, K.; Kück, U. Applied Microbiology and Biotechnology, Vol. 54, Issue 4 https://doi.org/10.1007/s002530000422	journal	October 2000
Endoplasmic Reticulum Stress-induced mRNA Splicing Permits Synthesis of Transcription Factor Hac1p/Ern4p That Activates the Unfolded Protein Response Kawahara, Tetsushi; Yanagi, Hideki; Yura, Takashi Molecular Biology of the Cell, Vol. 8, Issue 10 https://doi.org/10.1091/mbc.8.10.1845	journal	October 1997
Induction, isolation, and characterization of aspergillus niger mutant strains producing elevated levels of beta-galactosidase. Nevalainen, K. M. Applied and Environmental Microbiology, Vol. 41, Issue 3 https://doi.org/10.1128/AEM.41.3.593-596.1981	journal	January 1981
Molecular characterization of the Aspergillus nidulans fbxA encoding an F-box protein involved in xylanase induction Colabardini, Ana Cristina; Humanes, Ana Carolina; Gouvea, Paula Fagundes Fungal Genetics and Biology, Vol. 49, Issue 2 https://doi.org/10.1016/j.fgb.2011.11.004	journal	February 2012
Intensification of glucose oxidase synthesis by multistage mutagenesis ofAspergillus niger: Scientific note Tahoun, M. K. Applied Biochemistry and Biotechnology, Vol. 39-40, Issue 1 https://doi.org/10.1007/BF02918997	journal	September 1993
Selection to sequence: opportunities in fungal genomics Baker, Scott E. Environmental Microbiology, Vol. 11, Issue 12 https://doi.org/10.1111/j.1462-2920.2009.02112.x	journal	December 2009
Snf1-Like Protein Kinase Ssp2 Regulates Glucose Derepression in Schizosaccharomyces pombe Matsuzawa, Tomohiko; Fujita, Yasuko; Tohda, Hideki Eukaryotic Cell, Vol. 11, Issue 2 https://doi.org/10.1128/EC.05268-11	journal	December 2011
Mutants of Trichoderma reesei are defective in cellulose induction, but not basal expression of cellulase-encoding genes Torigoi, E.; Henrique-Silva, F.; Escobar-Vera, J. Gene, Vol. 173, Issue 2 https://doi.org/10.1016/0378-1119(96)00219-3	journal	September 1996
Genome sequencing and transcriptome analysis of Trichoderma reesei QM9978 strain reveals a distal chromosome translocation to be responsible for loss of vib1 expression and loss of cellulase induction Ivanova, Christa; Ramoni, Jonas; Aouam, Thiziri Biotechnology for Biofuels, Vol. 10, Issue 1 https://doi.org/10.1186/s13068-017-0897-7	journal	September 2017
The glucose repressor CRE1 from Sclerotinia sclerotiorum is functionally related to CREA from Aspergillus nidulans but not to the Mig proteins from Saccharomyces cerevisiae Vautard, Géraldine; Cotton, Pascale; Fèvre, Michel FEBS Letters, Vol. 453, Issue 1-2 https://doi.org/10.1016/S0014-5793(99)00691-2	journal	June 1999
The bZIP Transcription Factor HAC-1 Is Involved in the Unfolded Protein Response and Is Necessary for Growth on Cellulose in Neurospora crassa Montenegro-Montero, Alejandro; Goity, Alejandra; Larrondo, Luis F. PLOS ONE, Vol. 10, Issue 7 https://doi.org/10.1371/journal.pone.0131415	journal	July 2015
Activation of the unfolded protein response in Pichia pastoris requires splicing of a HAC1 mRNA intron and retention of the C-terminal tail of Hac1p Whyteside, Graham; Nor, Rohana Mat; Alcocer, Marcos J. C. FEBS Letters, Vol. 585, Issue 7 https://doi.org/10.1016/j.febslet.2011.02.036	journal	March 2011
Glucose uptake in germinating Aspergillus nidulans conidia: involvement of the creA and sorA genes MacCabe, A. P. Microbiology, Vol. 149, Issue 8 https://doi.org/10.1099/mic.0.26349-0	journal	August 2003
The transcription factor HACA mediates the unfolded protein response in Aspergillus niger , and up-regulates its own transcription Mulder, H. J.; Saloheimo, M.; Penttil�, M. Molecular Genetics and Genomics, Vol. 271, Issue 2 https://doi.org/10.1007/s00438-003-0965-5	journal	March 2004
The challenge of enzyme cost in the production of lignocellulosic biofuels Klein-Marcuschamer, Daniel; Oleskowicz-Popiel, Piotr; Simmons, Blake A. Biotechnology and Bioengineering, Vol. 109, Issue 4 https://doi.org/10.1002/bit.24370	journal	November 2011
Genome-wide analysis of the endoplasmic reticulum stress response during lignocellulase production in Neurospora crassa Fan, Feiyu; Ma, Guoli; Li, Jingen Biotechnology for Biofuels, Vol. 8, Issue 1 https://doi.org/10.1186/s13068-015-0248-5	journal	April 2015
Combining manipulation of transcription factors and overexpression of the target genes to enhance lignocellulolytic enzyme production in Penicillium oxalicum Gao, Liwei; Li, Zhonghai; Xia, Chengqiang Biotechnology for Biofuels, Vol. 10, Issue 1 https://doi.org/10.1186/s13068-017-0783-3	journal	April 2017
Constitutive Expression of Chimeric Transcription Factors Enables Cellulase Synthesis under Non-Inducing Conditions in Penicillium oxalicum Gao, Liwei; Xia, Chengqiang; Xu, Jiadi Biotechnology Journal, Vol. 12, Issue 11 https://doi.org/10.1002/biot.201700119	journal	September 2017
Synthetic Gene Network with Positive Feedback Loop Amplifies Cellulase Gene Expression in Neurospora crassa Matsu-ura, Toru; Dovzhenok, Andrey A.; Coradetti, Samuel T. ACS Synthetic Biology, Vol. 7, Issue 5 https://doi.org/10.1021/acssynbio.8b00011	journal	April 2018
F-Box Proteins Are Receptors that Recruit Phosphorylated Substrates to the SCF Ubiquitin-Ligase Complex Skowyra, Dorota; Craig, Karen L.; Tyers, Mike Cell, Vol. 91, Issue 2 https://doi.org/10.1016/S0092-8674(00)80403-1	journal	October 1997
Phosphorylation Positively Regulates DNA Binding of the Carbon Catabolite Repressor Cre1 of Hypocrea jecorina ( Trichoderma reesei ) Cziferszky, Angela; Mach, Robert L.; Kubicek, Christian P. Journal of Biological Chemistry, Vol. 277, Issue 17 https://doi.org/10.1074/jbc.M200744200	journal	February 2002
The fungus Aspergillus niger consumes sugars in a sequential manner that is not mediated by the carbon catabolite repressor CreA Mäkelä, Miia R.; Aguilar-Pontes, María Victoria; van Rossen-Uffink, Diana Scientific Reports, Vol. 8, Issue 1 https://doi.org/10.1038/s41598-018-25152-x	journal	April 2018
Development of a genome-editing CRISPR/Cas9 system in thermophilic fungal Myceliophthora species and its application to hyper-cellulase production strain engineering Liu, Qian; Gao, Ranran; Li, Jingen Biotechnology for Biofuels, Vol. 10, Issue 1 https://doi.org/10.1186/s13068-016-0693-9	journal	January 2017
Magnificent seven: roles of G protein-coupled receptors in extracellular sensing in fungi Xue, Chaoyang; Hsueh, Yen-Ping; Heitman, Joseph FEMS Microbiology Reviews, Vol. 32, Issue 6 https://doi.org/10.1111/j.1574-6976.2008.00131.x	journal	November 2008
Snf1 Protein Kinase Regulates Phosphorylation of the Mig1 Repressor in Saccharomyces cerevisiae Treitel, Michelle A.; Kuchin, Sergei; Carlson, Marian Molecular and Cellular Biology, Vol. 18, Issue 11 https://doi.org/10.1128/MCB.18.11.6273	journal	November 1998
Mutation of the Xylanase regulator 1 causes a glucose blind hydrolase expressing phenotype in industrially used Trichoderma strains Derntl, Christian; Gudynaite-Savitch, Loreta; Calixte, Sophie Biotechnology for Biofuels, Vol. 6, Issue 1 https://doi.org/10.1186/1754-6834-6-62	journal	January 2013
Similar is not the same: Differences in the function of the (hemi-)cellulolytic regulator XlnR (Xlr1/Xyr1) in filamentous fungi Klaubauf, Sylvia; Narang, Hari Mander; Post, Harm Fungal Genetics and Biology, Vol. 72 https://doi.org/10.1016/j.fgb.2014.07.007	journal	November 2014
The Aspergillus niger carbon catabolite repressor encoding gene, creA Drysdale, Mary R.; Kolze, Sharon E.; Kelly, Joan M. Gene, Vol. 130, Issue 2 https://doi.org/10.1016/0378-1119(93)90425-3	journal	August 1993
Sugar Transport in Neurospora crassa Scarborough, Gene A. Journal of Biological Chemistry, Vol. 245, Issue 7 https://doi.org/10.1016/S0021-9258(19)77148-8	journal	April 1970
Long-term strain improvements accumulate mutations in regulatory elements responsible for hyper-production of cellulolytic enzymes Liu, Guodong; Zhang, Lei; Qin, Yuqi Scientific Reports, Vol. 3, Issue 1 https://doi.org/10.1038/srep01569	journal	March 2013
Characterization of Cellulase Secretion and Cre1-Mediated Carbon Source Repression in the Potential Lignocellulose-Degrading Strain Trichoderma asperellum T-1 Wang, Qun; Lin, Hui; Shen, Qi PLOS ONE, Vol. 10, Issue 3 https://doi.org/10.1371/journal.pone.0119237	journal	March 2015
Improvement of cellulolytic enzyme production and performance by rational designing expression regulatory network and enzyme system composition Li, Zhonghai; Liu, Guodong; Qu, Yinbo Bioresource Technology, Vol. 245 https://doi.org/10.1016/j.biortech.2017.06.120	journal	December 2017
Deletion of homologs of the SREBP pathway results in hyper-production of cellulases in Neurospora crassa and Trichoderma reesei Reilly, Morgann C.; Qin, Lina; Craig, James P. Biotechnology for Biofuels, Vol. 8, Issue 1 https://doi.org/10.1186/s13068-015-0297-9	journal	August 2015
Forward genetics screen coupled with whole-genome resequencing identifies novel gene targets for improving heterologous enzyme production in Aspergillus niger Reilly, Morgann C.; Kim, Joonhoon; Lynn, Jed Applied Microbiology and Biotechnology, Vol. 102, Issue 4 https://doi.org/10.1007/s00253-017-8717-3	journal	January 2018
Restoration of female fertility in Trichoderma reesei QM6a provides the basis for inbreeding in this industrial cellulase producing fungus Linke, Rita; Thallinger, Gerhard G.; Haarmann, Thomas Biotechnology for Biofuels, Vol. 8, Issue 1 https://doi.org/10.1186/s13068-015-0311-2	journal	September 2015
Methods for genetic transformation of filamentous fungi Li, Dandan; Tang, Yu; Lin, Jun Microbial Cell Factories, Vol. 16, Issue 1 https://doi.org/10.1186/s12934-017-0785-7	journal	October 2017
Carbon catabolite repression in plant pathogenic fungi: isolation and characterization of the Gibberella fujikuroi and Botrytis cinerea creA genes Tudzynski, Bettina; Liu, Songjie; Kelly, Joan M. FEMS Microbiology Letters, Vol. 184, Issue 1 https://doi.org/10.1111/j.1574-6968.2000.tb08982.x	journal	March 2000
The low affinity glucose transporter HxtB is also involved in glucose signalling and metabolism in Aspergillus nidulans dos Reis, Thaila Fernanda; Nitsche, Benjamin M.; de Lima, Pollyne Borborema Almeida Scientific Reports, Vol. 7, Issue 1 https://doi.org/10.1038/srep45073	journal	March 2017
ER homeostasis and autophagy Smith, Matthew; Wilkinson, Simon Essays in Biochemistry, Vol. 61, Issue 6 https://doi.org/10.1042/EBC20170092	journal	December 2017
Mutation of CRE1 in Fusarium oxysporum reverts the pathogenicity defects of the FRP1 deletion mutant Jonkers, Wilfried; Rep, Martijn Molecular Microbiology, Vol. 74, Issue 5 https://doi.org/10.1111/j.1365-2958.2009.06922.x	journal	December 2009
Genetic Modification of Carbon Catabolite Repression in Trichoderma reesei for Improved Protein Production Nakari-Setala, T.; Paloheimo, M.; Kallio, J. Applied and Environmental Microbiology, Vol. 75, Issue 14 https://doi.org/10.1128/AEM.00282-09	journal	May 2009
Conserved RNA structures in the non-canonical Hac1/Xbp1 intron Hooks, Katarzyna B.; Griffiths-Jones, Sam RNA Biology, Vol. 8, Issue 4 https://doi.org/10.4161/rna.8.4.15396	journal	July 2011
Overexpressing key component genes of the secretion pathway for enhanced secretion of an Aspergillus niger glucose oxidase in Trichoderma reesei Wu, Yilan; Sun, Xianhua; Xue, Xianli Enzyme and Microbial Technology, Vol. 106 https://doi.org/10.1016/j.enzmictec.2017.07.007	journal	November 2017
Kinetic Characteristics of the Two Glucose Transport Systems in Neurospora crassa Schneider, R. P.; Wiley, W. R. Journal of Bacteriology, Vol. 106, Issue 2 https://doi.org/10.1128/JB.106.2.479-486.1971	journal	January 1971
A Novel Mechanism for Regulating Activity of a Transcription Factor That Controls the Unfolded Protein Response Cox, Jeffery S.; Walter, Peter Cell, Vol. 87, Issue 3 https://doi.org/10.1016/S0092-8674(00)81360-4	journal	November 1996
rco-3 , a Gene Involved in Glucose Transport and Conidiation in Neurospora crassa Madi, Lea; McBride, Sheila A.; Bailey, Lori A. Genetics, Vol. 146, Issue 2 https://doi.org/10.1093/genetics/146.2.499	journal	June 1997
Properties of the hexose transport systems of Aspergillus nidulans Mark, Cynthia G.; Romano, Antonio H. Biochimica et Biophysica Acta (BBA) - Biomembranes, Vol. 249, Issue 1 https://doi.org/10.1016/0005-2736(71)90098-8	journal	October 1971
Genome sequencing of the Trichoderma reesei QM9136 mutant identifies a truncation of the transcriptional regulator XYR1 as the cause for its cellulase-negative phenotype Lichius, Alexander; Bidard, Frédérique; Buchholz, Franziska BMC Genomics, Vol. 16, Issue 1 https://doi.org/10.1186/s12864-015-1526-0	journal	April 2015
Omics Analyses of Trichoderma reesei CBS999.97 and QM6a Indicate the Relevance of Female Fertility to Carbohydrate-Active Enzyme and Transporter Levels Tisch, Doris; Pomraning, Kyle R.; Collett, James R. Applied and Environmental Microbiology, Vol. 83, Issue 22 https://doi.org/10.1128/AEM.01578-17	journal	September 2017
Deciphering the Regulatory Network between the SREBP Pathway and Protein Secretion in Neurospora crassa Qin, Lina; Wu, Vincent W.; Glass, N. Louise mBio, Vol. 8, Issue 2 https://doi.org/10.1128/mBio.00233-17	journal	April 2017
Mutation of a putative AMPK phosphorylation site abolishes the repressor activity but not the nuclear targeting of the fungal glucose regulator CRE1 Vautard-Mey, Géraldine; Fèvre, Michel Current Genetics, Vol. 37, Issue 5 https://doi.org/10.1007/s002940050535	journal	May 2000
Comparative Genomics Analysis of Trichoderma reesei Strains Koike, Hideaki; Aerts, Andrea; LaButti, Kurt Industrial Biotechnology, Vol. 9, Issue 6 https://doi.org/10.1089/ind.2013.0015	journal	December 2013
A new Zn(II)2Cys6-type transcription factor BglR regulates β-glucosidase expression in Trichoderma reesei Nitta, Mikiko; Furukawa, Takanori; Shida, Yosuke Fungal Genetics and Biology, Vol. 49, Issue 5 https://doi.org/10.1016/j.fgb.2012.02.009	journal	May 2012
Aspergillus nidulans protein kinase A plays an important role in cellulase production de Assis, Leandro José; Ries, Laure Nicolas Annick; Savoldi, Marcela Biotechnology for Biofuels, Vol. 8, Issue 1 https://doi.org/10.1186/s13068-015-0401-1	journal	December 2015

Figures / Tables (1)

Fig. 1(p. 3)

Similar Records

Engineering Anaerobic Gut Fungi for Lignocellulose Breakdown

Technical Report · Mon Dec 10 00:00:00 EST 2018 · OSTI ID:1463032

O'Malley, Michelle Ann

Activity-based protein profiling of secreted cellulolytic enzyme activity dynamics in Trichoderma reesei QM6a, NG14, and RUT-C30

Journal Article · Sun Dec 01 00:00:00 EST 2013 · Molecular Biosystems, 9(12):2992-3000 · OSTI ID:1463032

Anderson, Lindsey N.; Culley, David E.; Hofstad, Beth A.; +7 more

Tracking the roots of cellulase hyperproduction by the fungus Trichoderma reesei using massively parallel DNA sequencing

Journal Article · Tue Sep 22 00:00:00 EDT 2009 · Proceedings of the National Academy of Sciences of the United States of America, 106(38):16151-16156 · OSTI ID:1463032

Le Crom, Stphane; Schackwitz, Wendy; Pennacchiod, Len; +14 more

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
60 APPLIED LIFE SCIENCES
Enzyme
Protein
Hyperproduction
Secretion
Biodesign
Fungi
Biotechnology
Enzyme
Protein
Hyperproduction
Secretion
Biodesign
Fungi
Biotechnology

Title: Protein hyperproduction in fungi by design

Citation Formats

References (96)

Figures / Tables (1)

Similar Records

Related Subjects