Zika virus detection using antibody-immobilized disposable cover glass and AlGaN/GaN high electron mobility transistors

Yang, Jiancheng; Carey, IV, Patrick; Ren, Fan; Mastro, Michael A.; Beers, Kimberly; Pearton, S. J.; Kravchenko, Ivan I.

doi:10.1063/1.5029902

Title: Zika virus detection using antibody-immobilized disposable cover glass and AlGaN/GaN high electron mobility transistors

Journal Article · Mon Jul 16 00:00:00 EDT 2018 · Applied Physics Letters

DOI:https://doi.org/10.1063/1.5029902· OSTI ID:1461056

Yang, Jiancheng ^[1];

^[1];

^[1]; Mastro, Michael A. ^[2]; Beers, Kimberly ^[1];

^[1];

^[3]

Univ. of Florida, Gainesville, FL (United States)
U.S. Naval Research Lab, Washington, D.C. (United States)
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)

Zika virus detection was demonstrated using antibody-functionalized cover glasses externally connected to the gate electrode of an AlGaN/GaN high electron mobility transistor (HEMT). A pulsed bias voltage of 0.5 V was applied to an electrode on the region of the cover glass region functionalized with antibody, and the resulting changes of drain current of the HEMT were employed to determine the presence of Zika virus antigen concentration ranging from 0.1 to 100 ng/ml. The dynamic and static drain current changes as a function of Zika virus concentration were modeled with a spring-like elastic relaxation model and the Langmuir extension model, respectively. Excellent fits to the data were found with relaxation time constants of antibody and antigen molecules in the range of 11 μs and 0.66–24.4 μs, respectively, for the concentration range investigated. The ratio of antibody bound with antigen to the total available antibody on the functionalized contact window was in the range of 0.013–0.84 for the Zika antigen concentration range of 0.1–100 ng/ml. Since the HEMT is not exposed to the bio-solution, it can be used repeatedly. The functionalized glass is the only disposable part in the detection system, showing the potential of this approach for hand-held, low cost sensor packages for point-of-care applications.

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Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE

Grant/Contract Number:: AC05-00OR22725

OSTI ID:: 1461056

Alternate ID(s):: OSTI ID: 1460502

Journal Information:: Applied Physics Letters, Vol. 113, Issue 3; ISSN 0003-6951

Publisher:: American Institute of Physics (AIP)Copyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 21 works

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