skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Unlocking the photobiological conversion of CO2 to (R)-3-hydroxybutyrate in cyanobacteria

Journal Article · · Green Chemistry
DOI:https://doi.org/10.1039/C8GC01208C· OSTI ID:1461075

Mitigation of a bottleneck significantly improved ( R )-3HB productivity, and metabolic flux analysis delineated dramatic metabolic flux changes in cyanobacterium Synechocystis .

Research Organization:
National Renewable Energy Lab. (NREL), Golden, CO (United States)
Sponsoring Organization:
USDOE Office of Energy Efficiency and Renewable Energy (EERE)
Grant/Contract Number:
AC36-08GO28308
OSTI ID:
1461075
Alternate ID(s):
OSTI ID: 1460895
Report Number(s):
NREL/JA-2700-71741; GRCHFJ
Journal Information:
Green Chemistry, Vol. 20, Issue 16; ISSN 1463-9262
Publisher:
Royal Society of ChemistryCopyright Statement
Country of Publication:
United States
Language:
English
Citation Metrics:
Cited by: 25 works
Citation information provided by
Web of Science

References (55)

Tailoring cyanobacterial cell factory for improved industrial properties journal March 2018
Fatty acid production in genetically modified cyanobacteria journal April 2011
Positive Regulation of psbA Gene Expression by cis-Encoded Antisense RNAs in Synechocystis sp. PCC 6803 journal August 2012
Microbial production and applications of chiral hydroxyalkanoates journal February 2005
INCA: a computational platform for isotopically non-stationary metabolic flux analysis journal January 2014
Metabolic engineering of cyanobacteria for the synthesis of commodity products journal June 2015
The γ-aminobutyric acid shunt contributes to closing the tricarboxylic acid cycle in S ynechocystis sp. PCC 6803 : The γ-aminobutyric acid shunt in journal July 2014
Metabolic engineering of Synechococcus sp. PCC 7002 to produce poly-3-hydroxybutyrate and poly-3-hydroxybutyrate-co-4-hydroxybutyrate journal November 2015
Biodegradable Polymers- A Review on Recent Trends and Emerging Perspectives journal June 2011
A Genetic Toolbox for Modulating the Expression of Heterologous Genes in the Cyanobacterium Synechocystis sp. PCC 6803 journal August 2017
Reconstruction and Comparison of the Metabolic Potential of Cyanobacteria Cyanothece sp. ATCC 51142 and Synechocystis sp. PCC 6803 journal October 2012
Biotechnological production of (R)-3-hydroxybutyric acid monomer journal November 2007
An AU-box motif upstream of the SD sequence of light-dependent psbA transcripts confers mRNA instability in darkness in cyanobacteria journal May 2001
13 C-Tracer and Gas Chromatography-Mass Spectrometry Analyses Reveal Metabolic Flux Distribution in the Oleaginous Microalga Chlorella protothecoides journal August 2010
13 C-MFA delineates the photomixotrophic metabolism of Synechocystis sp. PCC 6803 under light- and carbon-sufficient conditions journal April 2014
Biosynthesis of poly(glycolate-co-lactate-co-3-hydroxybutyrate) from glucose by metabolically engineered Escherichia coli journal May 2016
Engineering cyanobacteria for photosynthetic production of 3-hydroxybutyrate directly from CO2 journal March 2013
A photoautotrophic platform for the sustainable production of valuable plant natural products from CO 2 journal January 2016
Phosphoketolase pathway contributes to carbon metabolism in cyanobacteria journal December 2015
Dark-induced mRNA instability involves RNase E/G-type endoribonuclease cleavage at the AU-box and SD sequences in cyanobacteria journal July 2007
Enhanced poly-?-hydroxybutyrate accumulation in a unicellular cyanobacterium, Synechocystis sp. PCC 6803 journal February 2007
Evaluation of promoters and ribosome binding sites for biotechnological applications in the unicellular cyanobacterium Synechocystis sp. PCC 6803 journal November 2016
Antibiotics-free stable polyhydroxyalkanoate (PHA) production from carbon dioxide by recombinant cyanobacteria journal December 2011
Lactate fraction dependent mechanical properties of semitransparent poly(lactate-co-3-hydroxybutyrate)s produced by control of lactyl-CoA monomer fluxes in recombinant Escherichia coli journal July 2011
Polyhydroxyalkanoates, challenges and opportunities journal December 2014
Engineering the methylerythritol phosphate pathway in cyanobacteria for photosynthetic isoprene production from CO 2 journal January 2016
Evaluation of methods to extract and quantify lipids from Synechocystis PCC 6803 journal January 2011
Engineering microbial chemical factories to produce renewable “biomonomers” journal January 2012
Bacterial synthesis of biodegradable polyhydroxyalkanoates journal June 2007
The plasticity of cyanobacterial carbon metabolism journal December 2017
Microbial production of R-3-hydroxybutyric acid by recombinant E. coli harboring genes of phbA, phbB, and tesB journal July 2007
Quantitative target analysis and kinetic profiling of acyl-CoAs reveal the rate-limiting step in cyanobacterial 1-butanol production journal January 2016
Comonomer Unit Composition and Thermal Properties of Poly(3-hydroxybutyrate- c o -4-hydroxybutyrate)s Biosynthesized by Ralstonia e utropha journal December 2001
Methods for enhancing cyanobacterial stress tolerance to enable improved production of biofuels and industrially relevant chemicals journal January 2018
Cyanobacterial PHA Production—Review of Recent Advances and a Summary of Three Years’ Working Experience Running a Pilot Plant journal March 2017
Biofuel toxicity and mechanisms of biofuel tolerance in three model cyanobacteria journal July 2014
Cyanobacteria: Promising biocatalysts for sustainable chemical production journal October 2017
Microbial production of polyhydroxyalkanoates (PHAs) and its copolymers: A review of recent advancements journal August 2016
A Novel Paradigm of Fatty Acid β-Oxidation Exemplified by the Thioesterase-Dependent Partial Degradation of Conjugated Linoleic Acid That Fully Supports Growth of Escherichia coli journal September 2008
Analytics for Metabolic Engineering journal September 2015
A balanced ATP driving force module for enhancing photosynthetic biosynthesis of 3-hydroxybutyrate from CO 2 journal March 2018
One-step fermentative production of poly(lactate-co-glycolate) from carbohydrates in Escherichia coli journal March 2016
Oxygen-tolerant coenzyme A-acylating aldehyde dehydrogenase facilitates efficient photosynthetic n-butanol biosynthesis in cyanobacteria journal January 2013
Progress and challenges in producing polyhydroxyalkanoate biopolymers from cyanobacteria journal November 2016
Premethylation of Foreign DNA Improves Integrative Transformation Efficiency in Synechocystis sp. Strain PCC 6803 journal October 2015
Asymmetric hydrogenation of .beta.-keto carboxylic esters. A practical, purely chemical access to .beta.-hydroxy esters in high enantiomeric purity journal September 1987
Metabolic Engineering of Escherichia coli for Enhanced Production of (R)- and (S)-3-Hydroxybutyrate journal March 2009
Metabolic Engineering of Escherichia coli for Production of Enantiomerically Pure (R)-(-)-Hydroxycarboxylic Acids journal June 2003
Synthetic Biology Toolbox for Controlling Gene Expression in the Cyanobacterium Synechococcus sp. strain PCC 7002 journal September 2014
13C-based metabolic flux analysis journal May 2009
Cyanobacterial carbon metabolism: Fluxome plasticity and oxygen dependence: Cyanobacterial Carbon Metabolism journal March 2017
Enatiomerically pure hydroxycarboxylic acids: current approaches and future perspectives journal April 2010
The Tricarboxylic Acid Cycle in Cyanobacteria journal December 2011
Strategies for achieving high-level expression of genes in Escherichia coli. journal January 1996
Enatiomerically pure hydroxycarboxylic acids: Current approaches and future perspectives text January 2010

Cited By (3)

Applications of stable isotope-based metabolomics and fluxomics toward synthetic biology of cyanobacteria journal December 2019
Scale-up challenges and requirement of technology-transfer for cyanobacterial poly (3-hydroxybutyrate) production in industrial scale journal January 2019
Bioprocess Engineering Aspects of Sustainable Polyhydroxyalkanoate Production in Cyanobacteria journal December 2018