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Title: Glucan Binding Protein C of Streptococcus mutans Mediates both Sucrose-Independent and Sucrose-Dependent Adherence

Abstract

The high-resolution structure of glucan binding protein C (GbpC) at 1.14 Å, a sucrose-dependent virulence factor of the dental caries pathogenStreptococcus mutans, has been determined. GbpC shares not only structural similarities with the V regions of AgI/II and SspB but also functional adherence to salivary agglutinin (SAG) and its scavenger receptor cysteine-rich domains (SRCRs). This is not only a newly identified function for GbpC but also an additional fail-safe binding mechanism forS. mutans. Despite the structural similarities withS. mutansantigen I/II (AgI/II) and SspB ofStreptococcus gordonii, GbpC remains unique among these surface proteins in its propensity to adhere to dextran/glucans. The complex crystal structure of GbpC with dextrose (β-d-glucose; Protein Data Bank ligand BGC) highlights exclusive structural features that facilitate this interaction with dextran. Targeted deletion mutant studies on GbpC's divergent loop region in the vicinity of a highly conserved calcium binding site confirm its role in biofilm formation. Finally, we present a model for adherence to dextran. The structure of GbpC highlights how artfully microbes have engineered the lectin-like folds to broaden their functional adherence repertoire.

Authors:
; ; ; ; ; ; ; ;
Publication Date:
Research Org.:
Argonne National Lab. (ANL), Argonne, IL (United States). Advanced Photon Source (APS)
Sponsoring Org.:
National Institutes of Health (NIH)
OSTI Identifier:
1460844
Resource Type:
Journal Article
Journal Name:
Infection and Immunity
Additional Journal Information:
Journal Volume: 86; Journal Issue: 7; Journal ID: ISSN 0019-9567
Publisher:
American Society for Microbiology
Country of Publication:
United States
Language:
ENGLISH

Citation Formats

Mieher, Joshua L., Larson, Matthew R., Schormann, Norbert, Purushotham, Sangeetha, Wu, Ren, Rajashankar, Kanagalaghatta R., Wu, Hui, Deivanayagam, Champion, and Freitag, Nancy E. Glucan Binding Protein C of Streptococcus mutans Mediates both Sucrose-Independent and Sucrose-Dependent Adherence. United States: N. p., 2018. Web. doi:10.1128/IAI.00146-18.
Mieher, Joshua L., Larson, Matthew R., Schormann, Norbert, Purushotham, Sangeetha, Wu, Ren, Rajashankar, Kanagalaghatta R., Wu, Hui, Deivanayagam, Champion, & Freitag, Nancy E. Glucan Binding Protein C of Streptococcus mutans Mediates both Sucrose-Independent and Sucrose-Dependent Adherence. United States. doi:10.1128/IAI.00146-18.
Mieher, Joshua L., Larson, Matthew R., Schormann, Norbert, Purushotham, Sangeetha, Wu, Ren, Rajashankar, Kanagalaghatta R., Wu, Hui, Deivanayagam, Champion, and Freitag, Nancy E. Mon . "Glucan Binding Protein C of Streptococcus mutans Mediates both Sucrose-Independent and Sucrose-Dependent Adherence". United States. doi:10.1128/IAI.00146-18.
@article{osti_1460844,
title = {Glucan Binding Protein C of Streptococcus mutans Mediates both Sucrose-Independent and Sucrose-Dependent Adherence},
author = {Mieher, Joshua L. and Larson, Matthew R. and Schormann, Norbert and Purushotham, Sangeetha and Wu, Ren and Rajashankar, Kanagalaghatta R. and Wu, Hui and Deivanayagam, Champion and Freitag, Nancy E.},
abstractNote = {The high-resolution structure of glucan binding protein C (GbpC) at 1.14 Å, a sucrose-dependent virulence factor of the dental caries pathogenStreptococcus mutans, has been determined. GbpC shares not only structural similarities with the V regions of AgI/II and SspB but also functional adherence to salivary agglutinin (SAG) and its scavenger receptor cysteine-rich domains (SRCRs). This is not only a newly identified function for GbpC but also an additional fail-safe binding mechanism forS. mutans. Despite the structural similarities withS. mutansantigen I/II (AgI/II) and SspB ofStreptococcus gordonii, GbpC remains unique among these surface proteins in its propensity to adhere to dextran/glucans. The complex crystal structure of GbpC with dextrose (β-d-glucose; Protein Data Bank ligand BGC) highlights exclusive structural features that facilitate this interaction with dextran. Targeted deletion mutant studies on GbpC's divergent loop region in the vicinity of a highly conserved calcium binding site confirm its role in biofilm formation. Finally, we present a model for adherence to dextran. The structure of GbpC highlights how artfully microbes have engineered the lectin-like folds to broaden their functional adherence repertoire.},
doi = {10.1128/IAI.00146-18},
journal = {Infection and Immunity},
issn = {0019-9567},
number = 7,
volume = 86,
place = {United States},
year = {2018},
month = {4}
}

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