Method and apparatus for measuring biofilm thickness and topology
An apparatus and method of measuring biofilm and biological activity on a surface is disclosed. The apparatus includes a biofilm, which includes one or more microorganisms, grown on a substrate. A viewing window is placed on a surface of the biofilm and a gas bubble is introduced between the viewing window and the surface of the biofilm. The space between the substrate and the viewing window may be enclosed in a casing that has an inlet and an outlet, forming a flow cell. A microscope system, such as a white light interferometer, captures data of the biofilm in situ and non-destructively. The 3D images of biofilm surface have high resolution while maintaining a large field of view. The apparatuses and methods will be useful for fundamental studies of biofilms, biomedical and environmental screening, and many other applications in biology and the life sciences.
- Research Organization:
- Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)
- Sponsoring Organization:
- USDOE
- DOE Contract Number:
- AC0576RL01830
- Assignee:
- BATTELLE MEMORIAL INSTITUTE (Richland, WA)
- Patent Number(s):
- 10,005,999
- Application Number:
- 15/071,921
- OSTI ID:
- 1460555
- Resource Relation:
- Patent File Date: 2016 Mar 16
- Country of Publication:
- United States
- Language:
- English
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