Structural visualization of RNA polymerase III transcription machineries

Han, Yan; Yan, Chunli; Fishbain, Susan; Ivanov, Ivaylo; He, Yuan

doi:10.1038/s41421-018-0044-z

Structural visualization of RNA polymerase III transcription machineries

Journal Article · Tue Jul 31 04:00:00 EDT 2018 · Cell Discovery

DOI:https://doi.org/10.1038/s41421-018-0044-z· OSTI ID:1459999

; Yan, Chunli; Fishbain, Susan; ;

Abstract

RNA polymerase III (Pol III) transcription initiation requires the action of the transcription factor IIIB (TFIIIB) and is highly regulated. Here, we determine the structures of Pol III pre-initiation complexes (PICs) using single particle cryo-electron microscopy (cryo-EM). We observe stable Pol III–TFIIIB complexes using nucleic acid scaffolds mimicking various functional states, in which TFIIIB tightly encircles the upstream promoter DNA. There is an intricate interaction between TFIIIB and Pol III, which stabilizes the winged-helix domains of the C34 subunit of Pol III over the active site cleft. The architecture of Pol III PIC more resembles that of the Pol II PIC than the Pol I PIC. In addition, we also obtain a 3D reconstruction of Pol III in complex with TFIIIB using the elongation complex (EC) scaffold, shedding light on the mechanism of facilitated recycling of Pol III prior to transcription re-initiation.

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Sponsoring Organization:: USDOE

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 1459999

Alternate ID(s):: OSTI ID: 1543739

Journal Information:: Cell Discovery, Journal Name: Cell Discovery Journal Issue: 1 Vol. 4; ISSN 2056-5968

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United Kingdom

Language:: English

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