A Versatile Click-Compatible Monolignol Probe to Study Lignin Deposition in Plant Cell Walls

Pandey, Jyotsna L.; Wang, Bo; Diehl, Brett G.; Richard, Tom L.; Chen, Gong; Anderson, Charles T.; Zabotina, Olga A.

doi:10.1371/journal.pone.0121334

A Versatile Click-Compatible Monolignol Probe to Study Lignin Deposition in Plant Cell Walls

Journal Article · Fri Apr 17 00:00:00 EDT 2015 · PLoS ONE

DOI:https://doi.org/10.1371/journal.pone.0121334· OSTI ID:1459346

Pandey, Jyotsna L. ^[1]; Wang, Bo ^[2]; Diehl, Brett G. ^[2]; Richard, Tom L. ^[2]; Chen, Gong ^[2]; Anderson, Charles T. ^[2]; Zabotina, Olga A. ^[3]

The Pennsylvania State Univ., University Park, PA (United States); The Pennsylvania State University, University Park, Pennsylvania, USA
The Pennsylvania State Univ., University Park, PA (United States)
Iowa State Univ., Ames, IA (United States)

Here, lignin plays important structural and functional roles in plants by forming a hydrophobic matrix in secondary cell walls that enhances mechanical strength and resists microbial decay. While the importance of the lignin matrix is well documented and the biosynthetic pathways for monolignols are known, the process by which lignin precursors or monolignols are transported and polymerized to form this matrix remains a subject of considerable debate. In this study, we have synthesized and tested an analog of coniferyl alcohol that has been modified to contain an ethynyl group at the C-3 position. This modification enables fluorescent tagging and imaging of this molecule after its incorporation into plant tissue by click chemistry-assisted covalent labeling with a fluorescent azide dye, and confers a distinct Raman signature that could be used for Raman imaging. We found that this monolignol analog is incorporated into in vitro-polymerized dehydrogenation polymer (DHP) lignin and into root epidermal cell walls of 4-day-old Arabidopsis seedlings. Incorporation of the analog in stem sections of 6-week-old Arabidopsis thaliana plants and labeling with an Alexa-594 azide dye revealed the precise locations of new lignin polymerization. Results from this study indicate that this molecule can provide high-resolution localization of lignification during plant cell wall maturation and lignin matrix assembly.

View Accepted Manuscript (DOE)

Research Organization:: Energy Frontier Research Centers (EFRC) (United States). Center for Lignocellulose Structure and Formation (CLSF); The Pennsylvania State Univ., University Park, PA (United States)

Sponsoring Organization:: USDOE

Grant/Contract Number:: SC0001090

OSTI ID:: 1459346

Alternate ID(s):: OSTI ID: 1210586

Journal Information:: PLoS ONE, Journal Name: PLoS ONE Journal Issue: 4 Vol. 10; ISSN 1932-6203

Publisher:: Public Library of ScienceCopyright Statement

Country of Publication:: United States

Language:: English

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59 BASIC BIOLOGICAL SCIENCES
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A Versatile Click-Compatible Monolignol Probe to Study Lignin Deposition in Plant Cell Walls

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