Gut bacteria are rarely shared by co-hospitalized premature infants, regardless of necrotizing enterocolitis development

Raveh-Sadka, Tali; Thomas, Brian C.; Singh, Andrea; Firek, Brian; Brooks, Brandon; Castelle, Cindy J.; Sharon, Itai; Baker, Robyn; Good, Misty; Morowitz, Michael J.; Banfield, Jillian F.

doi:10.7554/eLife.05477

Gut bacteria are rarely shared by co-hospitalized premature infants, regardless of necrotizing enterocolitis development

Journal Article · Mon Mar 02 23:00:00 EST 2015 · eLife

DOI:https://doi.org/10.7554/eLife.05477· OSTI ID:1171761

Raveh-Sadka, Tali ^[1]; Thomas, Brian C. ^[1]; Singh, Andrea ^[1]; Firek, Brian ^[2]; ^[1]; Castelle, Cindy J. ^[1]; Sharon, Itai ^[1]; Baker, Robyn ^[3]; Good, Misty ^[4]; Morowitz, Michael J. ^[2]; Banfield, Jillian F. ^[1]

Department of Earth and Planetary Science, University of California, Berkeley, Berkeley, United States
Department of Surgery, University of Pittsburgh School of Medicine, Pittsburgh, United States
Division of Newborn Medicine, Children's Hospital of Pittsburgh and Magee-Womens Hospital of UPMC, Pittsburgh, United States
Division of Newborn Medicine, Children's Hospital of Pittsburgh and Magee-Womens Hospital of UPMC, Pittsburgh, United States, Department of Pediatrics, University of Pittsburgh School of Medicine, Pittsburgh, United States

Premature infants are highly vulnerable to aberrant gastrointestinal tract colonization, a process that may lead to diseases like necrotizing enterocolitis. Thus, spread of potential pathogens among hospitalized infants is of great concern. Here, we reconstructed hundreds of high-quality genomes of microorganisms that colonized co-hospitalized premature infants, assessed their metabolic potential, and tracked them over time to evaluate bacterial strain dispersal among infants. We compared microbial communities in infants who did and did not develop necrotizing enterocolitis. Surprisingly, while potentially pathogenic bacteria of the same species colonized many infants, our genome-resolved analysis revealed that strains colonizing each baby were typically distinct. In particular, no strain was common to all infants who developed necrotizing enterocolitis. The paucity of shared gut colonizers suggests the existence of significant barriers to the spread of bacteria among infants. Importantly, we demonstrate that strain-resolved comprehensive community analysis can be accomplished on potentially medically relevant time scales.

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Sponsoring Organization:: USDOE

Grant/Contract Number:: SC0004918

OSTI ID:: 1171761

Alternate ID(s):: OSTI ID: 1243127
OSTI ID: 1455039

Journal Information:: eLife, Journal Name: eLife Vol. 4; ISSN 2050-084X

Publisher:: eLife Sciences Publications, Ltd.Copyright Statement

Country of Publication:: United States

Language:: English

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