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Title: Lipid accumulation from glucose and xylose in an engineered, naturally oleaginous strain of Saccharomyces cerevisiae

Abstract

Saccharomyces cerevisiae, a well-known industrial yeast for alcoholic fermentation, is not historically known to accumulate lipids. Four S. cerevisiae strains used in industrial applications were screened for their ability to accumulate neutral lipids. Only one, D5A, was found to accumulate up to 20% dry cell weight (dcw) lipids. This strain was further engineered by knocking out ADP-activated serine/threonine kinase (SNF1) which increased lipid accumulation to 35% dcw lipids. In addition, we engineered D5A to utilize xylose and found that D5A accumulates up to 37% dcw lipids from xylose as the sole carbon source. Further we over-expressed different diacylglycerol acyltransferase (DGA1) genes and boosted lipid accumulation to 50%. Fatty acid speciation showed that 94% of the extracted lipids consisted of 5 fatty acid species, C16:0 (palmitic), C16:1n7 (palmitoleic), C18:0 (stearic), C18:1n7 (vaccenic), and C18:1n9 (oleic), while the relative distributions changed depending on growth conditions. In addition, this strain accumulated lipids concurrently with ethanol production.

Authors:
; ; ;
Publication Date:
Research Org.:
National Renewable Energy Lab. (NREL), Golden, CO (United States)
Sponsoring Org.:
USDOE Office of Energy Efficiency and Renewable Energy (EERE), Bioenergy Technologies Office (EE-3B)
OSTI Identifier:
1454909
Report Number(s):
NREL/JA-5100-71759
Journal ID: ISSN 2292-8782
DOE Contract Number:  
AC36-08GO28308
Resource Type:
Journal Article
Journal Name:
Biofuel Research Journal
Additional Journal Information:
Journal Volume: 5; Journal Issue: 2; Journal ID: ISSN 2292-8782
Publisher:
BRTeam
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; Saccharomyces cerevisiae; xylose; lipid; biofuels; ethanol; genetic engineering

Citation Formats

Knoshaug, Eric P., Van Wychen, Stefanie, Singh, Arjun, and Zhang, Min. Lipid accumulation from glucose and xylose in an engineered, naturally oleaginous strain of Saccharomyces cerevisiae. United States: N. p., 2018. Web. doi:10.18331/BRJ2018.5.2.3.
Knoshaug, Eric P., Van Wychen, Stefanie, Singh, Arjun, & Zhang, Min. Lipid accumulation from glucose and xylose in an engineered, naturally oleaginous strain of Saccharomyces cerevisiae. United States. doi:10.18331/BRJ2018.5.2.3.
Knoshaug, Eric P., Van Wychen, Stefanie, Singh, Arjun, and Zhang, Min. Fri . "Lipid accumulation from glucose and xylose in an engineered, naturally oleaginous strain of Saccharomyces cerevisiae". United States. doi:10.18331/BRJ2018.5.2.3.
@article{osti_1454909,
title = {Lipid accumulation from glucose and xylose in an engineered, naturally oleaginous strain of Saccharomyces cerevisiae},
author = {Knoshaug, Eric P. and Van Wychen, Stefanie and Singh, Arjun and Zhang, Min},
abstractNote = {Saccharomyces cerevisiae, a well-known industrial yeast for alcoholic fermentation, is not historically known to accumulate lipids. Four S. cerevisiae strains used in industrial applications were screened for their ability to accumulate neutral lipids. Only one, D5A, was found to accumulate up to 20% dry cell weight (dcw) lipids. This strain was further engineered by knocking out ADP-activated serine/threonine kinase (SNF1) which increased lipid accumulation to 35% dcw lipids. In addition, we engineered D5A to utilize xylose and found that D5A accumulates up to 37% dcw lipids from xylose as the sole carbon source. Further we over-expressed different diacylglycerol acyltransferase (DGA1) genes and boosted lipid accumulation to 50%. Fatty acid speciation showed that 94% of the extracted lipids consisted of 5 fatty acid species, C16:0 (palmitic), C16:1n7 (palmitoleic), C18:0 (stearic), C18:1n7 (vaccenic), and C18:1n9 (oleic), while the relative distributions changed depending on growth conditions. In addition, this strain accumulated lipids concurrently with ethanol production.},
doi = {10.18331/BRJ2018.5.2.3},
journal = {Biofuel Research Journal},
issn = {2292-8782},
number = 2,
volume = 5,
place = {United States},
year = {2018},
month = {6}
}