Unique aspects of fiber degradation by the ruminal ethanologen Ruminococcus albus 7 revealed by physiological and transcriptomic analysis

Christopherson, Melissa R.; Dawson, John A.; Stevenson, David M.; Cunningham, Andrew C.; Bramhacharya, Shanti; Weimer, Paul J.; Kendziorski, Christina; Suen, Garret

doi:10.1186/1471-2164-15-1066

Title: Unique aspects of fiber degradation by the ruminal ethanologen Ruminococcus albus 7 revealed by physiological and transcriptomic analysis

Journal Article · Thu Dec 04 00:00:00 EST 2014 · BMC Genomics

DOI:https://doi.org/10.1186/1471-2164-15-1066· OSTI ID:1454420

Christopherson, Melissa R. ^[1]; Dawson, John A. ^[2]; Stevenson, David M. ^[3]; Cunningham, Andrew C. ^[1]; Bramhacharya, Shanti ^[1]; Weimer, Paul J. ^[4]; Kendziorski, Christina ^[2]; Suen, Garret ^[1]

Univ. of Wisconsin, Madison, WI (United States). Dept. of Bacteriology
Univ. of Wisconsin, Madison, WI (United States). Dept. of Biostatistics and Medical Informatics
US Dept. of Agriculture-Agricultural Research Services (USDA-ARS), Madison, WI (United States). US Dairy Forage Research Center
Univ. of Wisconsin, Madison, WI (United States). Dept. of Bacteriology; US Dept. of Agriculture-Agricultural Research Services (USDA-ARS), Madison, WI (United States). US Dairy Forage Research Center

Bacteria in the genus Ruminococcus are ubiquitous members of the mammalian gastrointestinal tract. In particular, they are important in ruminants where they digest a wide range of plant cell wall polysaccharides. For example, Ruminococcus albus 7 is a primary cellulose degrader that produces acetate usable by its bovine host. Moreover, it is one of the few organisms that ferments cellulose to form ethanol at mesophilic temperatures in vitro. The mechanism of cellulose degradation by R. albus 7 is not well-defined and is thought to involve pilin-like proteins, unique carbohydrate-binding domains, a glycocalyx, and cellulosomes. We used a combination of comparative genomics, fermentation analyses, and transcriptomics to further clarify the cellulolytic and fermentative potential of R. albus 7. A comparison of the R. albus 7 genome sequence against the genome sequences of related bacteria that either encode or do not encode cellulosomes revealed that R. albus 7 does not encode for most canonical cellulosomal components. Fermentation analysis of R. albus 7 revealed the ability to produce ethanol and acetate on a wide range of fibrous substrates in vitro. Global transcriptomic analysis of R. albus 7 grown at identical dilution rates on cellulose and cellobiose in a chemostat showed that this bacterium, when growing on cellulose, utilizes a carbohydrate-degrading strategy that involves increased transcription of the rare carbohydrate-binding module (CBM) family 37 domain and the tryptophan biosynthetic operon. Our data suggest that R. albus 7 does not use canonical cellulosomal components to degrade cellulose, but rather up-regulates the expression of CBM37-containing enzymes and tryptophan biosynthesis. This study contributes to a revised model of carbohydrate degradation by this key member of the rumen ecosystem.

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Research Organization:: Univ. of Wisconsin, Madison, WI (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER); National Institutes of Health (NIH); USDA

Grant/Contract Number:: SC0008104; GM102756

OSTI ID:: 1454420

Journal Information:: BMC Genomics, Vol. 15, Issue 1; ISSN 1471-2164

Publisher:: SpringerCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 61 works

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Title: Unique aspects of fiber degradation by the ruminal ethanologen Ruminococcus albus 7 revealed by physiological and transcriptomic analysis

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