Observed metabolic asymmetry within soybean root nodules reflects unexpected complexity in rhizobacteria-legume metabolite exchange

Veličković, Dušan; Agtuca, Beverly J.; Stopka, Sylwia A.; Vertes, Akos; Koppenaal, David W.; Paša-Tolić, Ljiljana; Stacey, Gary; Anderton, Christopher R.

doi:10.1038/s41396-018-0188-8

Observed metabolic asymmetry within soybean root nodules reflects unexpected complexity in rhizobacteria-legume metabolite exchange

Journal Article · Wed Jun 13 00:00:00 EDT 2018 · The ISME Journal

DOI:https://doi.org/10.1038/s41396-018-0188-8· OSTI ID:1454346

Veličković, Dušan; Agtuca, Beverly J.; Stopka, Sylwia A.; Vertes, Akos; Koppenaal, David W.; Paša-Tolić, Ljiljana; Stacey, Gary;

Abstract

In this study, the three-dimensional spatial distributions of a number of metabolites involved in regulating symbiosis and biological nitrogen fixation (BNF) within soybean root nodules were revealed using matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI). While many metabolites exhibited distinct spatial compartmentalization, some metabolites were asymmetrically distributed throughout the nodule (e.g., S-adenosylmethionine). These results establish a more complex metabolic view of plant–bacteria symbiosis (and BNF) within soybean nodules than previously hypothesized. Collectively these findings suggest that spatial perspectives in metabolic regulation should be considered to unravel the overall complexity of interacting organisms, like those relating to associations of nitrogen-fixing bacteria with host plants.

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Sponsoring Organization:: USDOE

OSTI ID:: 1454346

Alternate ID(s):: OSTI ID: 1513206
OSTI ID: 1458774

Journal Information:: The ISME Journal, Journal Name: The ISME Journal Journal Issue: 9 Vol. 12; ISSN 1751-7362

Publisher:: Oxford University PressCopyright Statement

Country of Publication:: United Kingdom

Language:: English

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