Biopolymer analysis by laser ablation and mass spectrometry
- Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831-6378 (United States)
Various matrix materials were used for laser desorption of biological molecules, which include proteins and oligonucleotides. Both matrix-assisted laser desorption ionization (MALDI) and matrix-assisted laser desorption with post-ionization (MALPI) have been tried. Detection sensitivity of a few hundred femtomoles has been achieved by MALDI for oligomers and proteins. Both positive and negative ions were observed with comparative sizes of signals. No significant fragmentation was observed.
- Research Organization:
- Oak Ridge National Laboratory
- DOE Contract Number:
- AC05-84OR21400
- OSTI ID:
- 144882
- Report Number(s):
- CONF-9304144--
- Journal Information:
- AIP Conference Proceedings, Journal Name: AIP Conference Proceedings Journal Issue: 1 Vol. 288; ISSN APCPCS; ISSN 0094-243X
- Country of Publication:
- United States
- Language:
- English
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