Mechanistic insight into TRIP13-catalyzed Mad2 structural transition and spindle checkpoint silencing

Brulotte, Melissa L.; Jeong, Byung-Cheon; Li, Faxiang; Li, Bing; Yu, Eric B.; Wu, Qiong; Brautigam, Chad A.; Yu, Hongtao; Luo, Xuelian

doi:10.1038/s41467-017-02012-2

Title: Mechanistic insight into TRIP13-catalyzed Mad2 structural transition and spindle checkpoint silencing

Journal Article · Tue Dec 05 00:00:00 EST 2017 · Nature Communications

DOI:https://doi.org/10.1038/s41467-017-02012-2· OSTI ID:1440608

^[1]; Jeong, Byung-Cheon ^[1]; Li, Faxiang ^[1]; Li, Bing ^[1]; Yu, Eric B. ^[1]; Wu, Qiong ^[1]; Brautigam, Chad A. ^[1];

^[1]; Luo, Xuelian ^[1]

Univ. of Texas Southwestern Medical Center, Dallas, TX (United States)

The spindle checkpoint maintains genomic stability and prevents aneuploidy. Unattached kinetochores convert the latent open conformer of the checkpoint protein Mad2 (O-Mad2) to the active closed conformer (C-Mad2), bound to Cdc20. C-Mad2–Cdc20 is incorporated into the mitotic checkpoint complex (MCC), which inhibits the anaphase-promoting complex/cyclosome (APC/C). The C-Mad2-binding protein p31^comet and the ATPase TRIP13 promote MCC disassembly and checkpoint silencing. Here, using nuclear magnetic resonance (NMR) spectroscopy, we show that TRIP13 and p31^comet catalyze the conversion of C-Mad2 to O-Mad2, without disrupting its stably folded core. We determine the crystal structure of human TRIP13, and identify functional TRIP13 residues that mediate p31^comet–Mad2 binding and couple ATP hydrolysis to local unfolding of Mad2. TRIP13 and p31^cometprevent APC/C inhibition by MCC components, but cannot reactivate APC/C already bound to MCC. Therefore, TRIP13–p31^comet intercepts and disassembles free MCC not bound to APC/C through mediating the local unfolding of the Mad2 C-terminal region.

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Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States)

Sponsoring Organization:: USDOE; National Inst. of Health; Cancer Prevention and Research Inst. of Texas; Welch Foundation

Grant/Contract Number:: AC02-06CH11357; GM107415; RP160255; I-1932; I-1441

OSTI ID:: 1440608

Journal Information:: Nature Communications, Vol. 8, Issue 1; ISSN 2041-1723

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: ENGLISH

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Cited by: 30 works

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