Multiplexed targeted mass spectrometry assays for prostate cancer-associated urinary proteins

Journal Article · Tue Oct 10 00:00:00 EDT 2017 · Oncotarget

DOI:https://doi.org/10.18632/oncotarget.21710· OSTI ID:1439709

Shi, Tujin; Quek, Sue-Ing; Gao, Yuqian; Nicora, Carrie D.; Nie, Song; Fillmore, Thomas L.; Liu, Tao; Rodland, Karin D.; Smith, Richard D.; Leach, Robin J.; Thompson, Ian M.; Vitello, Elizabeth A.; Ellis, William J.; Liu, Alvin Y.; Qian, Wei-Jun

Biomarkers for effective early diagnosis and prognosis of prostate cancer are still lacking. Multiplexed assays for cancer-associated proteins could be useful for identifying biomarkers for cancer detection and stratification. Herein, we report the development of sensitive targeted mass spectrometry assays for simultaneous quantification of 10 prostate cancer-associated proteins in urine. The diagnostic utility of these markers was evaluated with an initial cohort of 20 clinical urine samples. Individual marker concentration was normalized against the measured urinary prostate-specific antigen level as a reference of prostate-specific secretion. The areas under the receiver-operating characteristic curves for the 10 proteins ranged from 0.75 for CXCL14 to 0.87 for CEACAM5. Furthermore, MMP9 level was found to be significantly higher in patients with high Gleason scores, suggesting a potential of MMP9 as a marker for risk level assessment. Taken together, our work illustrated the feasibility of accurate multiplexed measurements of low-abundance cancer-associated proteins in urine and provided a viable path forward for preclinical verification of candidate biomarkers for prostate cancer.

• Research Organization: Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Environmental Molecular Sciences Lab. (EMSL)
• Sponsoring Organization: USDOE
• DOE Contract Number: AC05-76RL01830
• OSTI ID: 1439709
• Report Number(s): PNNL-SA-128413; 48135; 453040075
• Journal Information: Oncotarget, Vol. 8, Issue 60; ISSN 1949-2553
• Publisher: Impact Journals
• Country of Publication: United States
• Language: English

Related Subjects

Environmental Molecular Sciences Laboratory