Molecular basis for the substrate specificity of quorum signal synthases

Dong, Shi-Hui; Frane, Nicole D.; Christensen, Quin H.; Greenberg, E. Peter; Nagarajan, Rajesh; Nair, Satish K.

doi:10.1073/pnas.1705400114

Title: Molecular basis for the substrate specificity of quorum signal synthases

Journal Article · Mon Aug 07 00:00:00 EDT 2017 · Proceedings of the National Academy of Sciences of the United States of America

DOI:https://doi.org/10.1073/pnas.1705400114· OSTI ID:1439614

^[1]; Frane, Nicole D. ^[2]; Christensen, Quin H. ^[3]; Greenberg, E. Peter ^[3]; Nagarajan, Rajesh ^[2]; Nair, Satish K. ^[1]

Univ. of Illinois at Urbana-Champaign, IL (United States)
Boise State Univ., ID (United States)
Univ. of Washington, Seattle, WA (United States)

In several Proteobacteria, LuxI-type enzymes catalyze the biosynthesis of acyl–homoserine lactones (AHL) signals using S-adenosyl–L-methionine and either cellular acyl carrier protein (ACP)-coupled fatty acids or CoA–aryl/acyl moieties as progenitors. Little is known about the molecular mechanism of signal biosynthesis, the basis for substrate specificity, or the rationale for donor specificity for any LuxI member. As such, here, we present several cocrystal structures of BjaI, a CoA-dependent LuxI homolog that represent views of enzyme complexes that exist along the reaction coordinate of signal synthesis. Complementary biophysical, structure–function, and kinetic analysis define the features that facilitate the unusual acyl conjugation with S-adenosylmethionine (SAM). We also identify the determinant that establishes specificity for the acyl donor and identify residues that are critical for acyl/aryl specificity. These results highlight how a prevalent scaffold has evolved to catalyze quorum signal synthesis and provide a framework for the design of small-molecule antagonists of quorum signaling.

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Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States). Advanced Photon Source (APS)

Sponsoring Organization:: National Institutes of Health (NIH)

Grant/Contract Number:: GM59026; GM117323

OSTI ID:: 1439614

Journal Information:: Proceedings of the National Academy of Sciences of the United States of America, Vol. 114, Issue 34; ISSN 0027-8424

Publisher:: National Academy of SciencesCopyright Statement

Country of Publication:: United States

Language:: ENGLISH

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Cited by: 40 works

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Title: Molecular basis for the substrate specificity of quorum signal synthases

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