Cyclotron Production of High–Specific Activity 55Co and In Vivo Evaluation of the Stability of 55Co Metal-Chelate-Peptide Complexes

Mastren, Tara; Marquez, Bernadette V.; Sultan, Deborah E.; Bollinger, Elizabeth; Eisenbeis, Paul; Voller, Tom; Lapi, Suzanne E.

doi:10.2310/7290.2015.00025

Cyclotron Production of High–Specific Activity ⁵⁵Co and In Vivo Evaluation of the Stability of ⁵⁵Co Metal-Chelate-Peptide Complexes

Journal Article · Thu Oct 01 00:00:00 EDT 2015 · Molecular Imaging

DOI:https://doi.org/10.2310/7290.2015.00025· OSTI ID:1439276

Mastren, Tara ^[1]; Marquez, Bernadette V. ^[2]; Sultan, Deborah E. ^[2]; Bollinger, Elizabeth ^[2]; Eisenbeis, Paul ^[2]; Voller, Tom ^[2]; Lapi, Suzanne E. ^[2]

Washington Univ., St. Louis, MO (United States); Washington Univ, Dept Radiol, Campus Box 8225,510 S Kingshighway Blvd, St Louis, MO 63110 USA
Washington Univ., St. Louis, MO (United States)

This work describes the production of high–specific activity ⁵⁵Co and the evaluation of the stability of ⁵⁵Co-metal-chelate-peptide complexes in vivo. ⁵⁵Co was produced via the ⁵⁸Ni(p,α)⁵⁵Co reaction and purified using anion exchange chromatography with an average recovery of 92% and an average specific activity of 1.96 GBq/μmol. ⁵⁵Co-DO3A and ⁵⁵Co-NO2A peptide complexes were radiolabeled at 3.7 MBq/μg and injected into HCT-116 tumor xenografted mice. Positron emission tomography (PET) and biodistribution studies were performed at 24 and 48 hours postinjection and compared to those of ⁵⁵CoCl². Both ⁵⁵Co-metal-chelate complexes demonstrated good in vivo stability by reducing the radiotracers’ uptake in the liver by sixfold at 24 hours with ˜ 1% ID/g and at 48 hours with ˜ 0.5% ID/g and reducing uptake in the heart by fourfold at 24 hours with ˜ 0.7% ID/g and sevenfold at 48 hours with ˜ 0.35% ID/g. Furthermore, these results support the use of ⁵⁵Co as a promising new radiotracer for PET imaging of cancer and other diseases.

View Accepted Manuscript (DOE)

Research Organization:: Washington Univ., St. Louis, MO (United States)

Sponsoring Organization:: USDOE Office of Science (SC)

Grant/Contract Number:: SC0007352

OSTI ID:: 1439276

Journal Information:: Molecular Imaging, Journal Name: Molecular Imaging Journal Issue: 10 Vol. 14; ISSN 1536-0121

Publisher:: SAGECopyright Statement

Country of Publication:: United States

Language:: English

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Radiometals for imaging and theranostics, current production, and future perspectives Mikolajczak, Renata; van der Meulen, Nicholas P.; Lapi, Suzanne E. Journal of Labelled Compounds and Radiopharmaceuticals, Vol. 62, Issue 10 https://doi.org/10.1002/jlcr.3770	journal	July 2019
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New 55Co-labeled Albumin-Binding Folate Derivatives as Potential PET Agents for Folate Receptor Imaging Radford, Lauren L.; Fernandez, Solana; Beacham, Rebecca Pharmaceuticals, Vol. 12, Issue 4 https://doi.org/10.3390/ph12040166	journal	November 2019
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