Building a genome engineering toolbox in nonmodel prokaryotic microbes

Freed, Emily; Fenster, Jacob; Smolinski, Sharon L.; Walker, Julie; Henard, Calvin A.; Gill, Ryan; Eckert, Carrie A.

doi:10.1002/bit.26727

Title: Building a genome engineering toolbox in nonmodel prokaryotic microbes

Journal Article · Fri May 11 00:00:00 EDT 2018 · Biotechnology and Bioengineering

DOI:https://doi.org/10.1002/bit.26727· OSTI ID:1439175

Freed, Emily ^[1]; Fenster, Jacob ^[2]; Smolinski, Sharon L. ^[3]; Walker, Julie ^[2]; Henard, Calvin A. ^[3];

^[1];

^[1]

National Renewable Energy Lab. (NREL), Golden, CO (United States); Univ. of Colorado, Boulder, CO (United States)
Univ. of Colorado, Boulder, CO (United States)
National Renewable Energy Lab. (NREL), Golden, CO (United States)

The realization of a sustainable bioeconomy requires our ability to understand and engineer complex design principles for the development of platform organisms capable of efficient conversion of cheap and sustainable feedstocks (e.g. sunlight, CO2, non-food biomass) to biofuels and bioproducts at sufficient titers and costs. For model microbes such as E. coli, advances in DNA reading and writing technologies are driving adoption of new paradigms for engineering biological systems. Unfortunately, microbes with properties of interest for the utilization of cheap and renewable feedstocks such as photosynthesis, autotrophic growth, and cellulose degradation have very few, if any, genetic tools for metabolic engineering. Therefore, it is important to begin to develop “design rules” for building a genetic toolbox for novel microbes. Here, we present an overview of our current understanding of these rules for the genetic manipulation of prokaryotic microbes and available genetic tools to expand our ability to genetically engineer non-model systems.

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Research Organization:: National Renewable Energy Laboratory (NREL), Golden, CO (United States); Univ. of Colorado, Boulder, CO (United States)

Sponsoring Organization:: USDOE Office of Energy Efficiency and Renewable Energy (EERE); USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: AC36-08GO28308; SC0018368

OSTI ID:: 1439175

Alternate ID(s):: OSTI ID: 1439401; OSTI ID: 1558846

Report Number(s):: NREL/JA-2700-71612

Journal Information:: Biotechnology and Bioengineering, Vol. 115, Issue 9; ISSN 0006-3592

Publisher:: WileyCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 16 works

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