Structure of a pentameric virion-associated fiber with a potential role in Orsay virus entry to host cells

Fan, Yanlin; Guo, Yusong R.; Yuan, Wang; Zhou, Ying; Holt, Matthew V.; Wang, Tao; Demeler, Borries; Young, Nicolas L.; Zhong, Weiwei; Tao, Yizhi J.

doi:10.1371/journal.ppat.1006231

Title: Structure of a pentameric virion-associated fiber with a potential role in Orsay virus entry to host cells

Journal Article · Mon Feb 27 00:00:00 EST 2017 · PLoS Pathogens

DOI:https://doi.org/10.1371/journal.ppat.1006231· OSTI ID:1438871

Fan, Yanlin ^[1]; Guo, Yusong R. ^[1]; Yuan, Wang ^[1]; Zhou, Ying ^[1];

^[2]; Wang, Tao ^[2]; Demeler, Borries ^[3];

^[2]; Zhong, Weiwei ^[1]; Tao, Yizhi J. ^[1]

Rice Univ., Houston, TX (United States)
Baylor College of Medicine, Houston, TX (United States)
Univ. of Texas Health Science Center at San Antonio, TX (United States)

Despite the wide use of Caenorhabditis elegans as a model organism, the first virus naturally infecting this organism was not discovered until six years ago. The Orsay virus and its related nematode viruses have a positive-sense RNA genome, encoding three proteins: CP, RdRP, and a novel δ protein that shares no homology with any other proteins. δ can be expressed either as a free δ or a CP-δ fusion protein by ribosomal frameshift, but the structure and function of both δ and CP-δ remain unknown. Using a combination of electron microscopy, X-ray crystallography, computational and biophysical analyses, here we show that the Orsay δ protein forms a ~420-Å long, pentameric fiber with an N-terminal α-helical bundle, a β-stranded filament in the middle, and a C-terminal head domain. The pentameric nature of the δ fiber has been independently confirmed by both mass spectrometry and analytical ultracentrifugation. Recombinant Orsay capsid containing CP-δ shows protruding long fibers with globular heads at the distal end. Mutant viruses with disrupted CP-δ fibers were generated by organism-based reverse genetics. These viruses were found to be either non-viable or with poor infectivity according to phenotypic and qRT-PCR analyses. Furthermore, addition of purified δ proteins to worm culture greatly reduced Orsay infectivity in a sequence-specific manner. Based on the structure resemblance between the Orsay CP-δ fiber and the fibers from reovirus and adenovirus, we propose that CP-δ functions as a cell attachment protein to mediate Orsay entry into worm intestine cells.

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Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States). Advanced Photon Source (APS)

Sponsoring Organization:: Robert A. Welch Foundation; Hamill Foundation; Kresge Science Initiative Endowment Fund at Rice University; National Institutes of Health (NIH); National Science Foundation (NSF)

Grant/Contract Number:: C-1565; R01-AI122356; P01-GM085354-08; R01-GM120600; NSF-ACI-1339649

OSTI ID:: 1438871

Journal Information:: PLoS Pathogens, Vol. 13, Issue 2; ISSN 1553-7374

Publisher:: Public Library of ScienceCopyright Statement

Country of Publication:: United States

Language:: ENGLISH

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Cited by: 10 works

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