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MtSWEET11, a Nodule-Specific Sucrose Transporter of Medicago truncatula

Journal Article · · Plant Physiology (Bethesda)
DOI:https://doi.org/10.1104/pp.15.01910· OSTI ID:1438839
 [1];  [2];  [2];  [3];  [2];  [4];  [4];  [5];  [3];  [2]
  1. The Samuel Roberts Foundation, Ardmore, OK (United States); Noble
  2. The Samuel Roberts Foundation, Ardmore, OK (United States)
  3. Carnegie Inst. of Science, Stanford, CA (United States)
  4. John Innes Centre, Norwich (United Kingdom)
  5. West Virginia Univ., Morgantown, WV (United States)
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Optimization of nitrogen fixation by rhizobia in legumes is a key area of research for sustainable agriculture. Symbiotic nitrogen fixation (SNF) occurs in specialized organs called nodules and depends on a steady supply of carbon to both plant and bacterial cells. Here we report the functional characterization of a nodule-specific Suc transporter, MtSWEET11 from Medicago truncatula. MtSWEET11 belongs to a clade of plant SWEET proteins that are capable of transporting Suc and play critical roles in pathogen susceptibility. When expressed in mammalian cells, MtSWEET11 transported sucrose (Suc) but not glucose (Glc). The MtSWEET11 gene was found to be expressed in infected root hair cells, and in the meristem, invasion zone, and vasculature of nodules. Expression of an MtSWEET11-GFP fusion protein in nodules resulted in green fluorescence associated with the plasma membrane of uninfected cells and infection thread and symbiosome membranes of infected cells. Two independent Tnt1-insertion sweet11 mutants were uncompromised in SNF. Furthermore, although MtSWEET11 appears to be involved in Suc distribution within nodules, it is not crucial for SNF, probably because other Suc transporters can fulfill its role(s).
Research Organization:
Carnegie Inst. of Science, Stanford, CA (United States)
Sponsoring Organization:
USDOE
Grant/Contract Number:
FG02-04ER15542
OSTI ID:
1438839
Journal Information:
Plant Physiology (Bethesda), Journal Name: Plant Physiology (Bethesda) Journal Issue: 1 Vol. 171; ISSN 0032-0889
Publisher:
American Society of Plant BiologistsCopyright Statement
Country of Publication:
United States
Language:
English

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NIN Acts as a Network Hub Controlling a Growth Module Required for Rhizobial Infection journal February 2019
Tea plant SWEET transporters: expression profiling, sugar transport, and the involvement of CsSWEET16 in modifying cold tolerance in Arabidopsis journal April 2018
Structure, evolution and diverse physiological roles of SWEET sugar transporters in plants journal April 2019
The relationship between root exudation properties and root morphological traits of cucumber grown under different nitrogen supplies and atmospheric CO2 concentrations journal February 2018
A first glimpse at genes important to the Azolla–Nostoc symbiosis journal January 2019
Molecular Characterization of LjSWEET3, a Sugar Transporter in Nodules of Lotus japonicus journal December 2016
Role of Cytosolic, Tyrosine-Insensitive Prephenate Dehydrogenase in Medicago truncatula journal September 2019
Exocytic trafficking pathways in plants: why and how they are redirected journal May 2017
Nitric oxide and phytoglobin PHYTOGB1 are regulatory elements in the Solanum lycopersicum – Rhizophagus irregularis mycorrhizal symbiosis journal May 2019
A Medicago truncatula SWEET transporter implicated in arbuscule maintenance during arbuscular mycorrhizal symbiosis journal June 2019
Auxin signalling of Arachis hypogaea activated by colonization of mutualistic fungus Phomopsis liquidambari enhances nodulation and N 2 -fixation: Auxin in fungus-legume-rhizobium interactions journal April 2018
Nutrient demand and fungal access to resources control the carbon allocation to the symbiotic partners in tripartite interactions of Medicago truncatula: Carbon allocation in tripartite interactions journal August 2018
Magnesium promotes root nodulation through facilitation of carbohydrate allocation in soybean journal July 2018
Sugars en route to the roots. Transport, metabolism and storage within plant roots and towards microorganisms of the rhizosphere journal September 2018
An improved method for Agrobacterium rhizogenes-mediated transformation of tomato suitable for the study of arbuscular mycorrhizal symbiosis journal May 2018
Sugar transporters in Fabaceae, featuring SUT MST and SWEET families of the model plant Medicago truncatula and the agricultural crop Pisum sativum journal September 2019
The Symbiosome: Legume and Rhizobia Co-evolution toward a Nitrogen-Fixing Organelle? journal January 2018
The Expanded SWEET Gene Family Following Whole Genome Triplication in Brassica rapa journal September 2019
Regulation of Symbiotic Nitrogen Fixation in Legume Root Nodules journal September 2019
SWEET Gene Family in Medicago truncatula: Genome-Wide Identification, Expression and Substrate Specificity Analysis journal September 2019

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