Resolution extension by image summing in serial femtosecond crystallography of two-dimensional membrane-protein crystals
Previous proof-of-concept measurements on single-layer two-dimensional membrane-protein crystals performed at X-ray free-electron lasers (FELs) have demonstrated that the collection of meaningful diffraction patterns, which is not possible at synchrotrons because of radiation-damage issues, is feasible. Here, the results obtained from the analysis of a thousand single-shot, room-temperature X-ray FEL diffraction images from two-dimensional crystals of a bacteriorhodopsin mutant are reported in detail. The high redundancy in the measurements boosts the intensity signal-to-noise ratio, so that the values of the diffracted intensities can be reliably determined down to the detector-edge resolution of 4 Å. The results show that two-dimensional serial crystallography at X-ray FELs is a suitable method to study membrane proteins to near-atomic length scales at ambient temperature. The method presented here can be extended to pump–probe studies of optically triggered structural changes on submillisecond timescales in two-dimensional crystals, which allow functionally relevant large-scale motions that may be quenched in three-dimensional crystals.
- Research Organization:
- Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
- Sponsoring Organization:
- USDOE National Nuclear Security Administration (NNSA)
- Grant/Contract Number:
- AC52-07NA27344
- OSTI ID:
- 1437805
- Alternate ID(s):
- OSTI ID: 1557945
- Report Number(s):
- LLNL-JRNL-782409; IUCRAJ; PII: S2052252517017043
- Journal Information:
- IUCrJ, Journal Name: IUCrJ Vol. 5 Journal Issue: 1; ISSN 2052-2525
- Publisher:
- International Union of Crystallography (IUCr)Copyright Statement
- Country of Publication:
- United Kingdom
- Language:
- English
Web of Science
Resolution extension by image summing in serial femtosecond crystallography of two-dimensional membrane-protein crystals
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text | January 2018 |
A first attempt investigation on crystallization screening and crystal quality of lysozyme under different simulated gravities in a large-gradient magnetic field
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journal | January 2019 |
Structure-factor amplitude reconstruction from serial femtosecond crystallography of two-dimensional membrane-protein crystals
|
journal | January 2019 |
A fixed-target platform for serial femtosecond crystallography in a hydrated environment
|
journal | January 2020 |
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