Preliminary neutron diffraction analysis of challenging human manganese superoxide dismutase crystals

Azadmanesh, Jahaun; Trickel, Scott R.; Weiss, Kevin L.; Coates, Leighton; Borgstahl, Gloria E. O.

doi:10.1107/S2053230X17003508

Title: Preliminary neutron diffraction analysis of challenging human manganese superoxide dismutase crystals

Journal Article · Wed Mar 29 00:00:00 EDT 2017 · Acta Crystallographica. Section F, Structural Biology Communications

DOI:https://doi.org/10.1107/S2053230X17003508· OSTI ID:1437709

Azadmanesh, Jahaun; Trickel, Scott R.;

;

; Borgstahl, Gloria E. O.

Superoxide dismutases (SODs) are enzymes that protect against oxidative stress by dismutation of superoxide into oxygen and hydrogen peroxide through cyclic reduction and oxidation of the active-site metal. The complete enzymatic mechanisms of SODs are unknown since data on the positions of hydrogen are limited. Here, methods are presented for large crystal growth and neutron data collection of human manganese SOD (MnSOD) using perdeuteration and the MaNDi beamline at Oak Ridge National Laboratory. The crystal from which the human MnSOD data set was obtained is the crystal with the largest unit-cell edge (240 Å) from which data have been collected via neutron diffraction to sufficient resolution (2.30 Å) where hydrogen positions can be observed.

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Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES); National Aeronautics and Space Administration (NASA); National Institutes of Health (NIH)

Grant/Contract Number:: AC05-00OR22725; 44-0307-1021-201; P30CA036727; 5P20RR016469

OSTI ID:: 1437709

Alternate ID(s):: OSTI ID: 1407982

Journal Information:: Acta Crystallographica. Section F, Structural Biology Communications, Journal Name: Acta Crystallographica. Section F, Structural Biology Communications Vol. 73 Journal Issue: 4; ISSN 2053-230X

Publisher:: International Union of Crystallography (IUCr)Copyright Statement

Country of Publication:: United Kingdom

Language:: English

Citation Metrics:

Cited by: 15 works

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Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
60 APPLIED LIFE SCIENCES
manganese superoxide dismutase
neutron diffraction
perdeuteration
human
large unit cell

Title: Preliminary neutron diffraction analysis of challenging human manganese superoxide dismutase crystals

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References (26)

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