Crystal Structure of Cocosin, A Potential Food Allergen from Coconut (Cocos nucifera)

Jin, Tengchuan; Wang, Cheng; Zhang, Caiying; Wang, Yang; Chen, Yu-Wei; Guo, Feng; Howard, Andrew; Cao, Min-Jie; Fu, Tong-Jen; McHugh, Tara H.; Zhang, Yuzhu

doi:10.1021/acs.jafc.7b02252

Crystal Structure of Cocosin, A Potential Food Allergen from Coconut (Cocos nucifera)

Journal Article · Mon Jul 31 00:00:00 EDT 2017 · Journal of Agricultural and Food Chemistry

DOI:https://doi.org/10.1021/acs.jafc.7b02252· OSTI ID:1437479

^[1]; Wang, Cheng ^[2]; Zhang, Caiying ^[3]; Wang, Yang ^[4]; Chen, Yu-Wei ^[4]; Guo, Feng ^[4]; Howard, Andrew ^[4]; Cao, Min-Jie ^[5]; Fu, Tong-Jen ^[6]; McHugh, Tara H. ^[7]; ^[8]

Univ. of Science and Technology of China, Hefei (China); Illinois Inst. of Technology, Chicago, IL (United States); Jimei Univ., Xiamen, FJ (China)
Univ. of Science and Technology of China, Hefei (China); Jimei Univ., Xiamen, FJ (China)
Univ. of Science and Technology of China, Hefei (China)
Illinois Inst. of Technology, Chicago, IL (United States)
Jimei Univ., Xiamen, FJ (China)
US Food and Drug Administration, Bedford Park, IL (United States). Division of Food Processing Science and Technology
Agricultural Research Service (USDA-ARS), Albany, CA (United States). Western Regional Research Center, Healthy Processed Foods Research Unit
Illinois Inst. of Technology, Chicago, IL (United States); Agricultural Research Service (USDA-ARS), Albany, CA (United States). Western Regional Research Center, Healthy Processed Foods Research Unit

Coconut (Cocos nucifera) is an important palm tree. Coconut fruit is widely consumed. The most abundant storage protein in coconut fruit is cocosin (a likely food allergen), which belongs to the 11S globulin family. Cocosin was crystallized near a century ago, but its structure remains unknown. By optimizing crystallization conditions and cryoprotectant solutions, we were able to obtain cocosin crystals that diffracted to 1.85 Å. The cocosin gene was cloned from genomic DNA isolated from dry coconut tissue. The protein sequence deduced from the predicted cocosin coding sequence was used to guide model building and structure refinement. We report the structure of cocosin was determined for the first time, and it revealed a typical 11S globulin feature of a double layer doughnut-shaped hexamer.

View Accepted Manuscript (DOE)

Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States). Advanced Photon Source (APS)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22); Illinois Institute of Technology; US Food and Drug Administration (FDA); Fundamental Research Funds for the Central Universities; Chinese Academy of Sciences (CAS)

Grant/Contract Number:: W-31109-ENG-38

OSTI ID:: 1437479

Journal Information:: Journal of Agricultural and Food Chemistry, Journal Name: Journal of Agricultural and Food Chemistry Journal Issue: 34 Vol. 65; ISSN 0021-8561

Publisher:: American Chemical SocietyCopyright Statement

Country of Publication:: United States

Language:: ENGLISH

References (51)

Crystal Structure of Soybean Proglycinin A1aB1b Homotrimer Adachi, Motoyasu; Takenaka, Yasuyuki; Gidamis, Andrew B. Journal of Molecular Biology, Vol. 305, Issue 2 https://doi.org/10.1006/jmbi.2000.4310	journal	January 2001
The Molecular Basis of Peanut Allergy Mueller, Geoffrey A.; Maleki, Soheila J.; Pedersen, Lars C. Current Allergy and Asthma Reports, Vol. 14, Issue 5 https://doi.org/10.1007/s11882-014-0429-5	journal	March 2014
The structure, physical and chemical properties of the soy bean protein glycinin Badley, R. A.; Atkinson, D.; Hauser, H. Biochimica et Biophysica Acta (BBA) - Protein Structure, Vol. 412, Issue 2 https://doi.org/10.1016/0005-2795(75)90036-7	journal	December 1975
The interpretation of protein structures: Estimation of static accessibility Lee, B.; Richards, F. M. Journal of Molecular Biology, Vol. 55, Issue 3 https://doi.org/10.1016/0022-2836(71)90324-X	journal	February 1971
Characterisation and crystallisation of an 11S seed storage globulin from coconut (Cocos nucifera) Carr, H. J.; Plumb, G. W.; Parker, M. L. Food Chemistry, Vol. 38, Issue 1 https://doi.org/10.1016/0308-8146(90)90202-F	journal	January 1990
[20] Processing of X-ray diffraction data collected in oscillation mode Otwinowski, Zbyszek; Minor, Wladek Macromolecular Crystallography Part A, 307-326 https://doi.org/10.1016/S0076-6879(97)76066-X	book	January 1997
Systemic allergic reaction to coconut (Cocos nucifera) in 2 subjects with hypersensitivity to tree nut and demonstration of cross-reactivity to legumin-like seed storage proteins: New coconut and walnut food allergens Teuber, Suzanne S.; Peterson, W. Rich Journal of Allergy and Clinical Immunology, Vol. 103, Issue 6 https://doi.org/10.1016/S0091-6749(99)70196-X	journal	June 1999
Kernel-specific cDNA clones encoding three different isoforms of seed storage protein glutelin from oil palm Elaeis guineensis Cha, T. S.; Habib Shah, Farida Plant Science, Vol. 160, Issue 5 https://doi.org/10.1016/S0168-9452(01)00335-1	journal	April 2001
Identification of a 7S globulin as a novel coconut allergen Benito, Cristina; González-Mancebo, Eloína; Díaz de Durana, M. Dolores Alonso Annals of Allergy, Asthma & Immunology, Vol. 98, Issue 6 https://doi.org/10.1016/S1081-1206(10)60739-9	journal	June 2007
Cross-reactivity between coconut and hazelnut proteins in a patient with coconut anaphylaxis Nguyen, Steven A.; More, Daniel R.; Whisman, Bonnie A. Annals of Allergy, Asthma & Immunology, Vol. 92, Issue 2 https://doi.org/10.1016/S1081-1206(10)61562-1	journal	February 2004
Purification and crystallization of coconut globulin cocosin from Cocos nucifera Balasundaresan, D.; Sugadev, R.; Ponnuswamy, M. N. Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics, Vol. 1601, Issue 1 https://doi.org/10.1016/S1570-9639(02)00401-6	journal	November 2002
Coconut anaphylaxis: Case report and review Michavila Gomez, A.; Amat Bou, M.; Gonzalez Cortés, M. V. Allergologia et Immunopathologia, Vol. 43, Issue 2 https://doi.org/10.1016/j.aller.2013.09.004	journal	March 2015
Association of tree nut and coconut sensitizations Polk, Brooke I.; Dinakarpandian, Deendayal; Nanda, Maya Annals of Allergy, Asthma & Immunology, Vol. 117, Issue 4 https://doi.org/10.1016/j.anai.2016.07.023	journal	October 2016
Conservation and divergence on plant seed 11S globulins based on crystal structures Tandang-Silvas, Mary Rose G.; Fukuda, Takako; Fukuda, Chisato Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics, Vol. 1804, Issue 7 https://doi.org/10.1016/j.bbapap.2010.02.016	journal	July 2010
Crystal structure of a major seed storage protein, 11S proglobulin, from Amaranthus hypochondriacus: Insight into its physico-chemical properties Tandang-Silvas, Mary Rose; Cabanos, Cerrone S.; Carrazco Peña, Laura Denisse Food Chemistry, Vol. 135, Issue 2 https://doi.org/10.1016/j.foodchem.2012.04.135	journal	November 2012
Crystal structure of Ara h 3, a major allergen in peanut Jin, Tengchuan; Guo, Feng; Chen, Yu-wei Molecular Immunology, Vol. 46, Issue 8-9 https://doi.org/10.1016/j.molimm.2009.01.023	journal	May 2009
Characterization of a cashew allergen, 11S globulin (Ana o 2), conformational epitope Robotham, Jason M.; Xia, Lixin; Willison, LeAnna N. Molecular Immunology, Vol. 47, Issue 9 https://doi.org/10.1016/j.molimm.2009.12.009	journal	May 2010
Conformational epitope mapping of Pru du 6, a major allergen from almond nut Willison, LeAnna N.; Zhang, Qian; Su, Mengna Molecular Immunology, Vol. 55, Issue 3-4 https://doi.org/10.1016/j.molimm.2013.02.004	journal	October 2013
Purification and Characterization of a Black Walnut ( Juglans nigra) Allergen, Jug n 4 Zhang, Yu-Zhu; Du, Wen-Xian; Fan, Yuting Journal of Agricultural and Food Chemistry, Vol. 65, Issue 2 https://doi.org/10.1021/acs.jafc.6b04387	journal	January 2017
Mining Novel Allergens from Coconut Pollen Employing Manual De Novo Sequencing and Homology-Driven Proteomics Saha, Bodhisattwa; Sircar, Gaurab; Pandey, Naren Journal of Proteome Research, Vol. 14, Issue 11 https://doi.org/10.1021/acs.jproteome.5b00657	journal	October 2015
The Molecular Weight of Cocosin Sjögren, Bertil; Spychalski, Romuald Journal of the American Chemical Society, Vol. 52, Issue 11 https://doi.org/10.1021/ja01374a031	journal	November 1930
11S and 7S Globulins of Coconut ( Cocos nucifera L.): Purification and Characterization ^† Garcia, Roberta N.; Arocena, Rambo V.; Laurena, Antonio C. Journal of Agricultural and Food Chemistry, Vol. 53, Issue 5 https://doi.org/10.1021/jf0402173	journal	March 2005
Jug r 4, a Legumin Group Food Allergen from Walnut ( Juglans regia Cv. Chandler) Wallowitz, Mikhael; Peterson, W. Rich; Uratsu, Sandie Journal of Agricultural and Food Chemistry, Vol. 54, Issue 21 https://doi.org/10.1021/jf061329s	journal	October 2006
Cloning and Characterization of an 11S Legumin, Car i 4, a Major Allergen in Pecan Sharma, Girdhari M.; Irsigler, Andre; Dhanarajan, Pushparani Journal of Agricultural and Food Chemistry, Vol. 59, Issue 17 https://doi.org/10.1021/jf2017447	journal	September 2011
Almond Allergens: Molecular Characterization, Detection, and Clinical Relevance Costa, Joana; Mafra, Isabel; Carrapatoso, Isabel Journal of Agricultural and Food Chemistry, Vol. 60, Issue 6 https://doi.org/10.1021/jf2044923	journal	February 2012
Purification, Crystallization and Preliminary X-ray Characterization of Prunin-1, a Major Component of the Almond ( Prunus dulcis ) Allergen Amandin Albillos, Silvia M.; Jin, Tengchuan; Howard, Andrew Journal of Agricultural and Food Chemistry, Vol. 56, Issue 13 https://doi.org/10.1021/jf800529k	journal	June 2008
Purification and Characterization of the 7S Vicilin from Korean Pine ( Pinus koraiensis ) Jin, Tengchuan; Albillos, Silvia M.; Chen, Yu-Wei Journal of Agricultural and Food Chemistry, Vol. 56, Issue 17 https://doi.org/10.1021/jf801138q	journal	September 2008
Crystal Structure of Prunin-1, a Major Component of the Almond ( Prunus dulcis ) Allergen Amandin Jin, Tengchuan; Albillos, Silvia M.; Guo, Feng Journal of Agricultural and Food Chemistry, Vol. 57, Issue 18 https://doi.org/10.1021/jf9017355	journal	September 2009
Anaphylaxis to coconut Rosado, A.; Fernández-Rivas, M.; González-Mancebo, E. Allergy, Vol. 57, Issue 2 https://doi.org/10.1034/j.1398-9995.2002.1n3476.x	journal	February 2002
A case of coconut allergy Tella, R.; Gaig, P.; Lombardero, M. Allergy, Vol. 58, Issue 8 https://doi.org/10.1034/j.1398-9995.2003.00213.x	journal	August 2003
Peanut allergen Ara h 3: Isolation from peanuts and biochemical characterization Koppelman, S. J.; Knol, E. F.; Vlooswijk, R. A. A. Allergy, Vol. 58, Issue 11 https://doi.org/10.1034/j.1398-9995.2003.00259.x	journal	November 2003
Electrostatics of nanosystems: Application to microtubules and the ribosome Baker, N. A.; Sept, D.; Joseph, S. Proceedings of the National Academy of Sciences, Vol. 98, Issue 18, p. 10037-10041 https://doi.org/10.1073/pnas.181342398	journal	August 2001
MEGA5: Molecular Evolutionary Genetics Analysis Using Maximum Likelihood, Evolutionary Distance, and Maximum Parsimony Methods Tamura, K.; Peterson, D.; Peterson, N. Molecular Biology and Evolution, Vol. 28, Issue 10 https://doi.org/10.1093/molbev/msr121	journal	May 2011
PDB2PQR: an automated pipeline for the setup of Poisson-Boltzmann electrostatics calculations Dolinsky, T. J.; Nielsen, J. E.; McCammon, J. A. Nucleic Acids Research, Vol. 32, Issue Web Server, p. W665-W667 https://doi.org/10.1093/nar/gkh381	journal	July 2004
Deciphering key features in protein structures with the new ENDscript server Robert, Xavier; Gouet, Patrice Nucleic Acids Research, Vol. 42, Issue W1 https://doi.org/10.1093/nar/gku316	journal	April 2014
Likelihood-enhanced fast rotation functions Storoni, Laurent C.; McCoy, Airlie J.; Read, Randy J. Acta Crystallographica Section D Biological Crystallography, Vol. 60, Issue 3 https://doi.org/10.1107/S0907444903028956	journal	February 2004
Coot model-building tools for molecular graphics Emsley, Paul; Cowtan, Kevin Acta Crystallographica Section D Biological Crystallography, Vol. 60, Issue 12, p. 2126-2132 https://doi.org/10.1107/S0907444904019158	journal	November 2004
Automated and accurate deposition of structures solved by X-ray diffraction to the Protein Data Bank Yang, Huanwang; Guranovic, Vladimir; Dutta, Shuchismita Acta Crystallographica Section D Biological Crystallography, Vol. 60, Issue 10 https://doi.org/10.1107/S0907444904019419	journal	September 2004
Likelihood-enhanced fast translation functions McCoy, Airlie J.; Grosse-Kunstleve, Ralf W.; Storoni, Laurent C. Acta Crystallographica Section D Biological Crystallography, Vol. 61, Issue 4 https://doi.org/10.1107/S0907444905001617	journal	March 2005
Optimal description of a protein structure in terms of multiple groups undergoing TLS motion Painter, Jay; Merritt, Ethan A. Acta Crystallographica Section D Biological Crystallography, Vol. 62, Issue 4 https://doi.org/10.1107/S0907444906005270	journal	March 2006
MolProbity : all-atom structure validation for macromolecular crystallography Chen, Vincent B.; Arendall, W. Bryan; Headd, Jeffrey J. Acta Crystallographica Section D Biological Crystallography, Vol. 66, Issue 1 https://doi.org/10.1107/S0907444909042073	journal	December 2009
XDS Kabsch, Wolfgang Acta Crystallographica Section D Biological Crystallography, Vol. 66, Issue 2 https://doi.org/10.1107/S0907444909047337	journal	January 2010
PHENIX: a comprehensive Python-based system for macromolecular structure solution Adams, Paul D.; Afonine, Pavel V.; Bunkóczi, Gábor Acta Crystallographica Section D Biological Crystallography, Vol. 66, Issue 2, p. 213-221 https://doi.org/10.1107/S0907444909052925	journal	January 2010
Refinement of Macromolecular Structures by the Maximum-Likelihood Method Murshudov, G. N.; Vagin, A. A.; Dodson, E. J. Acta Crystallographica Section D Biological Crystallography, Vol. 53, Issue 3 https://doi.org/10.1107/S0907444996012255	journal	May 1997
Purification, crystallization and initial crystallographic characterization of brazil-nut allergen Ber e 2 Guo, Feng; Jin, Tengchuan; Howard, Andrew Acta Crystallographica Section F Structural Biology and Crystallization Communications, Vol. 63, Issue 11 https://doi.org/10.1107/S1744309107051445	journal	October 2007
Identification of two pistachio allergens, Pis v 1 and Pis v 2, belonging to the 2S albumin and 11S globulin family Ahn, K.; Bardina, L.; Grishina, G. Clinical & Experimental Allergy, Vol. 39, Issue 6 https://doi.org/10.1111/j.1365-2222.2009.03259.x	journal	June 2009
Cross-reactivity between coconut and lentil related to a 7S globulin and an 11S globulin: ALLERGYNet Manso, L.; Pastor, C.; Pérez-Gordo, M. Allergy, Vol. 65, Issue 11 https://doi.org/10.1111/j.1398-9995.2010.02370.x	journal	April 2010
Peanut Allergy, Allergen Composition, and Methods of Reducing Allergenicity: A Review Zhou, Yang; Wang, Jin-shui; Yang, Xiao-jia International Journal of Food Science, Vol. 2013 https://doi.org/10.1155/2013/909140	journal	January 2013
Relevant B Cell Epitopes in Allergic Disease Pomés, Anna International Archives of Allergy and Immunology, Vol. 152, Issue 1 https://doi.org/10.1159/000260078	journal	January 2010
Cloning, Expression and Patient IgE Reactivity of Recombinant Pru du 6, an 11S Globulin from Almond Willison, LeAnna N.; Tripathi, Pallavi; Sharma, Girdhari International Archives of Allergy and Immunology, Vol. 156, Issue 3 https://doi.org/10.1159/000323887	journal	January 2011
Further studies on the biological activity of hazelnut allergens Blanc, F.; Bernard, H.; Ah‐Leung, S. Clinical and Translational Allergy, Vol. 5, Issue 1 https://doi.org/10.1186/s13601-015-0066-7	journal	January 2015

Cited By (1)

Structural basis for the formation of soy protein nanofibrils Josefsson, Leila; Cronhamn, Melker; Ekman, Malin RSC Advances, Vol. 9, Issue 11 https://doi.org/10.1039/c8ra10610j	journal	January 2019

Similar Records

Purification, Crystallization and Preliminary X-ray Characterization of Prunin-1, a Major Component of the Almond (Prunus dulcis) Allergen Amandin

Journal Article · Mon Aug 04 00:00:00 EDT 2008 · J. Agric. Food Chem. · OSTI ID:1006746

Crystal Structure of Prunin-1, a Major Component of the Almond (Prunus dulcis) Allergen Amandin

Journal Article · Thu Oct 28 00:00:00 EDT 2010 · J. Agric. Food Chem. · OSTI ID:1006198

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
Coconut
Cocosin
Crystal structure
Food
Crystals
Immunology
Peptides and proteins

Crystal Structure of Cocosin, A Potential Food Allergen from Coconut (Cocos nucifera)

Citation Formats

References (51)

Cited By (1)

Similar Records

Related Subjects