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Title: Hierarchical molecular dynamics of bovine serum albumin in concentrated aqueous solution below and above thermal denaturation

Journal Article · · Physical Chemistry Chemical Physics. PCCP
DOI:https://doi.org/10.1039/c4cp04944f· OSTI ID:1436055
 [1];  [2];  [1];  [3];  [4]; ORCiD logo [5];  [6];  [4];  [2]
  1. Institut Max von Laue - Paul Langevin (ILL), Grenoble (France); Univ. Tubingen, Tubingen (Germany)
  2. Institut Max von Laue - Paul Langevin (ILL), Grenoble (France)
  3. Univ. Tubingen, Tubingen (Germany); Max-Planck Institute for Developmental Biology, Tubingen (Germany)
  4. Univ. Tubingen, Tubingen (Germany)
  5. Forschungszentrum Julich, Julich (Germany); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
  6. Forschungszentrum Julich, Julich (Germany)

Quasi-elastic neutron scattering provides a detailed microscopic picture of the global and internal protein dynamics reflecting thermally induced structural changes.

Research Organization:
Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
Sponsoring Organization:
USDOE
Grant/Contract Number:
AC05-00OR22725
OSTI ID:
1436055
Journal Information:
Physical Chemistry Chemical Physics. PCCP, Vol. 17, Issue 6; ISSN 1463-9076
Publisher:
Royal Society of ChemistryCopyright Statement
Country of Publication:
United States
Language:
English
Citation Metrics:
Cited by: 39 works
Citation information provided by
Web of Science

References (76)

Dynamic Transition Associated with the Thermal Denaturation of a Small Beta Protein journal November 2002
Evolution of the Internal Dynamics of Two Globular Proteins from Dry Powder to Solution journal July 1999
A physical insight into the elastic incoherent structure factor journal December 1992
A Nuclear Magnetic Resonance study of the reversible denaturation of hydrated lysozyme journal August 2011
Protein self-diffusion in crowded solutions journal July 2011
Temperature Dependence of Fast Dynamics in Proteins journal March 2007
Biophysical Study of Thermal Denaturation of Apo-Calmodulin: Dynamics of Native and Unfolded States journal December 2008
Picosecond dynamical changes on denaturation of yeast phosphoglycerate kinase revealed by quasielastic neutron scattering journal July 1997
In Vivo Measurement of Internal and Global Macromolecular Motions in Escherichia coli journal July 2008
A Measure of Conformational Entropy Change during Thermal Protein Unfolding Using Neutron Spectroscopy journal June 2003
The study of biological structures by neutron scattering from solution journal October 1976
Kinetics of Aggregation and Gelation of Globular Proteins after Heat-Induced Denaturation journal September 1999
Theory of cooperative transitions in protein molecules. II. Phase diagram for a protein molecule in solution journal October 1989
Scaling Behavior and Structure of Denatured Proteins journal July 2005
Theory of cooperative transitions in protein molecules. I. Why denaturation of globular protein is a first-order phase transition journal October 1989
Denaturation of Bovine Serum Albumin (BSA) and Ovalbumin by High Pressure, Heat and Chemicals journal March 1992
Dynamics of well-folded and natively disordered proteins in solution: a time-of-flight neutron scattering study journal January 2008
Unaffected microscopic dynamics of macroscopically arrested water in dilute clay gels journal December 2008
Self-assembly of biopolymeric structures below the threshold of random cross-link percolation journal January 1996
Raman studies of bovine serum albumin journal January 1976
Thermally denatured proteins. Nuclear magnetic resonance, binding isotherm and chemical modification studies of thermally denatured bovine serum albumin journal January 1976
Thermal Fluctuations in Amphipol A8-35 Particles: A Neutron Scattering and Molecular Dynamics Study journal October 2014
Gaussian Model for Localized Translational Motion:  Application to Incoherent Neutron Scattering journal June 2006
Aggregation kinetics of bovine serum albumin studied by FTIR spectroscopy and light scattering journal February 2004
Observation of high-temperature dynamic crossover in protein hydration water and its relation to reversible denaturation of lysozyme journal April 2009
How Soft Is a Protein? A Protein Dynamics Force Constant Measured by Neutron Scattering journal June 2000
Globular protein gelation—theory and experiment journal July 2001
Theory of the Phase Transition between Helix and Random Coil in Polypeptide Chains journal August 1959
Conformational changes involved in thermal aggregation processes of bovine serum albumin journal August 2003
Protein dynamics studied by neutron scattering journal November 2002
Dynamical changes of hemoglobin and its surrounding water during thermal denaturation as studied by quasielastic neutron scattering and temperature modulated differential scanning calorimetry journal June 2008
Toward an outline of the topography of a realistic protein-folding funnel. journal April 1995
Hydrodynamic Radii of Native and Denatured Proteins Measured by Pulse Field Gradient NMR Techniques journal December 1999
Structures of bovine, equine and leporine serum albumin journal September 2012
Characterization of sub-nanosecond dynamics of the molten globule state of α-lactalbumin using quasielastic neutron scattering and molecular dynamics simulations journal August 2003
Dynamics of hard-sphere suspensions journal July 1994
Incoherent neutron scattering functions for random jump diffusion in bounded and infinite media journal February 1981
Neutron incoherent scattering law for diffusion in a potential of spherical symmetry: general formalism and application to diffusion inside a sphere journal October 1980
Dominant forces in protein folding journal August 1990
A time-of-flight backscattering spectrometer at the Spallation Neutron Source, BASIS journal August 2011
Prediction of protein function from protein sequence and structure journal January 1999
Conformation Changes of Proteins journal February 1954
Dynamics of highly concentrated protein solutions around the denaturing transition journal January 2012
Molecular crowding enhances native structure and stability of  / protein flavodoxin journal November 2007
A view of dynamics changes in the molten globule-native folding step by quasielastic neutron scattering 1 1Edited by P. E. Wright journal August 2000
Role of the solvent in the dynamical transitions of proteins: The case of the lysozyme-water system journal July 2007
Picosecond dynamics in haemoglobin from different species: A quasielastic neutron scattering study journal October 2014
Thermal aggregation of bovine serum albumin at different pH: comparison with human serum albumin journal July 2007
Combining structure and dynamics: non-denaturing high-pressure effect on lysozyme in solution journal June 2009
Heat-Induced Gelation of Globular Proteins. 1. Model for the Effects of Time and Temperature on the Gelation Time of BSA Gels journal August 1997
Temperature Dependence of the Internal Dynamics of a Calmodulin−Peptide Complex journal November 2002
Diffusion and Dynamics of γ-Globulin in Crowded Aqueous Solutions journal June 2014
Dynamical and structural modifications of staphylococcal nuclease on C-terminal truncation journal May 1999
Dynamic regimes and correlated structural dynamics in native and denatured alpha-lactalbumin 1 1Edited by M. F. Moody journal September 2001
Structural dynamics of supercooled water from quasielastic neutron scattering and molecular simulations journal April 2011
Radially Softening Diffusive Motions in a Globular Protein journal September 2001
Using polarization analysis to separate the coherent and incoherent scattering from protein samples journal January 2010
Heat-Induced Secondary Structure and Conformation Change of Bovine Serum Albumin Investigated by Fourier Transform Infrared Spectroscopy journal September 2004
Critical behaviour for vulcanization processes journal January 1977
H/D Isotope Effects in Protein Thermal Denaturation: The Case of Bovine Serum Albumin journal March 2011
Molecular dynamics and protein function journal May 2005
Protein folding and misfolding journal December 2003
Globular protein gelation journal July 2000
Dynamics of different functional parts of bacteriorhodopsin: H-2H labeling and neutron scattering journal April 1998
X-ray and light scattering study of the structure of large protein aggregates at neutral pH journal March 2005
Study of thermally and chemically unfolded conformations of bovine serum albumin by means of dynamic light scattering journal July 2008
Dynamics and Rigidity in an Intrinsically Disordered Protein, β-Casein journal June 2014
Mean-squared atomic displacements in hydrated lysozyme, native and denatured journal January 2010
Thermal Motions in Bacteriorhodopsin at Different Hydration Levels Studied by Neutron Scattering: Correlation with Kinetics and Light-Induced Conformational Changes journal October 1998
From Powder to Solution: Hydration Dependence of Human Hemoglobin Dynamics Correlated to Body Temperature journal June 2009
Temperature Behaviour of Human Serum Albumin journal March 1980
From Levinthal to pathways to funnels journal January 1997
Consistent Picture of the Reversible Thermal Unfolding of Hen Egg-White Lysozyme from Experiment and Molecular Dynamics journal October 2010
VMD: Visual molecular dynamics journal February 1996
Temperature Induced Morphological Transitions from Native to Unfolded Aggregated States of Human Serum Albumin journal June 2014
Protein diffusion in crowded electrolyte solutions journal January 2010

Cited By (11)

Phase transformations of bovine serum albumin: Evidences from Rayleigh‐Brillouin light scattering journal January 2019
Dynamics of proteins in solution journal January 2019
Thermally induced conformational changes and protein–protein interactions of bovine serum albumin in aqueous solution under different pH and ionic strengths as revealed by SAXS measurements journal January 2017
Homogeneous and heterogeneous dynamics in native and denatured bovine serum albumin journal January 2018
Macromolecular crowding and the importance of proper hydration for the structure and dynamics of protein solutions journal January 2018
Localised contacts lead to nanosecond hinge motions in dimeric bovine serum albumin journal January 2019
The first study on the impact of osmolytes in whole cells of high temperature-adapted microorganisms journal January 2019
Effect of Phosphorylation on a Human-like Osteopontin Peptide journal April 2017
Neutron spectroscopy on protein solutions employing backscattering with an increased energy range journal June 2019
Microscopic diffusion processes measured in living planarians journal March 2018
Dynamical Behavior of Human α-Synuclein Studied by Quasielastic Neutron Scattering journal April 2016