Combined Falling Drop/Open Port Sampling Interface System for Automated Flow Injection Mass Spectrometry

doi:https://doi.org/10.1021/acs.analchem.7b03899

Title: Combined Falling Drop/Open Port Sampling Interface System for Automated Flow Injection Mass Spectrometry

Abstract

The aim of this work was to demonstrate and to evaluate the analytical performance of a combined falling drop/open port sampling interface (OPSI) system as a simple noncontact, no-carryover, automated system for flow injection analysis with mass spectrometry. The falling sample drops were introduced into the OPSI using a widely available autosampler platform utilizing low cost disposable pipet tips and conventional disposable microtiter well plates. The volume of the drops that fell onto the OPSI was in the 7–15 μL range with an injected sample volume of several hundred nanoliters. Sample drop height, positioning of the internal capillary on the sampling end of the probe, and carrier solvent flow rate were optimized for maximum signal. Sample throughput, signal reproducibility, matrix effects, and quantitative analysis capability of the system were established using the drug molecule propranolol and its isotope labeled internal standard in water, unprocessed river water and two commercially available buffer matrices. A sample-to-sample throughput of ~45 s with a ~4.5 s base-to-base flow injection peak profile was obtained in these experiments. In addition, quantitation with minimally processed rat plasma samples was demonstrated with three different statin drugs (atorvastatin, rosuvastatin, and fluvastatin). Direct characterization capability of unprocessed samples was demonstratedmore »« less

Authors:

^[1];

^[1]; Orcutt, Matt ^[2]; Bentley, Adam ^[3]; Glick, Jim ^[3]; Flarakos, Jimmy ^[3]

Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Resolution Labs, New Haven, IN (United States)
Novartis Inst. for Biomedical Sciences Drug Metabolism & Pharmacokinetics, East Hanover, NJ (United States)

Publication Date:: 2017-11-07

Research Org.:: Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)

Sponsoring Org.:: USDOE Office of Science (SC), Basic Energy Sciences (BES)

OSTI Identifier:: 1435291

Grant/Contract Number:: AC05-00OR22725

Resource Type:: Journal Article: Accepted Manuscript

Journal Name:: Analytical Chemistry

Additional Journal Information:: Journal Volume: 89; Journal Issue: 22; Journal ID: ISSN 0003-2700

Publisher:: American Chemical Society (ACS)

Country of Publication:: United States

Language:: English

Subject:: 47 OTHER INSTRUMENTATION

Citation Formats


                    Van Berkel, Gary J., Kertesz, Vilmos, Orcutt, Matt, Bentley, Adam, Glick, Jim, and Flarakos, Jimmy. Combined Falling Drop/Open Port Sampling Interface System for Automated Flow Injection Mass Spectrometry.  United States: N. p., 2017. 
        Web.  doi:10.1021/acs.analchem.7b03899.

Copy to clipboard


                    Van Berkel, Gary J., Kertesz, Vilmos, Orcutt, Matt, Bentley, Adam, Glick, Jim, & Flarakos, Jimmy. Combined Falling Drop/Open Port Sampling Interface System for Automated Flow Injection Mass Spectrometry.  United States.  https://doi.org/10.1021/acs.analchem.7b03899

Copy to clipboard


                    Van Berkel, Gary J., Kertesz, Vilmos, Orcutt, Matt, Bentley, Adam, Glick, Jim, and Flarakos, Jimmy. Tue .  
        "Combined Falling Drop/Open Port Sampling Interface System for Automated Flow Injection Mass Spectrometry".  United States.  https://doi.org/10.1021/acs.analchem.7b03899.  https://www.osti.gov/servlets/purl/1435291.

Copy to clipboard


                    
@article{osti_1435291,

  title        = {Combined Falling Drop/Open Port Sampling Interface System for Automated Flow Injection Mass Spectrometry},

  author       = {Van Berkel, Gary J. and Kertesz, Vilmos and Orcutt, Matt and Bentley, Adam and Glick, Jim and Flarakos, Jimmy},

  abstractNote = {The aim of this work was to demonstrate and to evaluate the analytical performance of a combined falling drop/open port sampling interface (OPSI) system as a simple noncontact, no-carryover, automated system for flow injection analysis with mass spectrometry. The falling sample drops were introduced into the OPSI using a widely available autosampler platform utilizing low cost disposable pipet tips and conventional disposable microtiter well plates. The volume of the drops that fell onto the OPSI was in the 7–15 μL range with an injected sample volume of several hundred nanoliters. Sample drop height, positioning of the internal capillary on the sampling end of the probe, and carrier solvent flow rate were optimized for maximum signal. Sample throughput, signal reproducibility, matrix effects, and quantitative analysis capability of the system were established using the drug molecule propranolol and its isotope labeled internal standard in water, unprocessed river water and two commercially available buffer matrices. A sample-to-sample throughput of ~45 s with a ~4.5 s base-to-base flow injection peak profile was obtained in these experiments. In addition, quantitation with minimally processed rat plasma samples was demonstrated with three different statin drugs (atorvastatin, rosuvastatin, and fluvastatin). Direct characterization capability of unprocessed samples was demonstrated by the analysis of neat vegetable oils. Employing the autosampler system for spatially resolved liquid extraction surface sampling exemplified by the analysis of propranolol and its hydroxypropranolol glucuronide phase II metabolites from a rat thin tissue section was also illustrated.},

  doi          = {10.1021/acs.analchem.7b03899},

  url          = {https://www.osti.gov/biblio/1435291},
  journal      = {Analytical Chemistry},

  issn         = {0003-2700},

  number       = 22,

  volume       = 89,

  place        = {United States},

  year         = {2017},

  month        = {11}

}

Copy to clipboard

Journal Article:

Free Publicly Available Full Text

Accepted Manuscript (DOE)

Publisher's Version of Record

https://doi.org/10.1021/acs.analchem.7b03899

Other availability

Search WorldCat to find libraries that may hold this journal

Save / Share:

Export Metadata

Save to My Library

Works referencing / citing this record:

Solid phase microextraction coupled to mass spectrometry via a microfluidic open interface for rapid therapeutic drug monitoring
journal, January 2019

Looby, Nikita T.; Tascon, Marcos; Acquaro, Vinicius R.
The Analyst, Vol. 144, Issue 12
https://doi.org/10.1039/c9an00041k

Similar records in OSTI.GOV collections:

Automated Liquid Microjunction Surface Sampling-HPLC-MS/MS Analysis of Drugs and Metabolites in Whole-Body Thin Tissue Sections

Journal Article Kertesz, Vilmos ; Van Berkel, Gary J - Bioanalysis

A fully automated liquid extraction-based surface sampling system utilizing a commercially available autosampler coupled to high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) detection is reported. Discrete spots selected for droplet-based sampling and automated sample queue generation for both the autosampler and MS were enabled by using in-house developed software. In addition, co-registration of spatially resolved sampling position and HPLC-MS information to generate heatmaps of compounds monitored for subsequent data analysis was also available in the software. The system was evaluated with whole-body thin tissue sections from propranolol dosed rat. The hands-free operation of the system was demonstrated by creating heatmapsmore »« less
https://doi.org/10.4155/bio.13.42
Liquid Microjunction Surface Sampling Coupled with High-Pressure Liquid Chromatography-Electrospray Ionization-Mass Spectrometry for Analysis of Drugs and Metabolites in Whole-Body Thin Tissue Sections

Journal Article Kertesz, Vilmos ; Van Berkel, Gary J - Analytical Chemistry

In this work, a commercially available autosampler was adapted to perform direct liquid microjunction (LMJ) surface sampling followed by a high-pressure liquid chromatography (HPLC) separation of the extract components and detection with electrospray ionization mass spectrometry (ESI-MS). To illustrate the utility of coupling a separation with this direct liquid extraction based surface sampling approach, four different organs (brain, lung, kidney, and liver) from whole-body thin tissue sections of propranolol dosed and control mice were examined. The parent drug was observed in the chromatograms of the surface sampling extracts from all the organs of the dosed mouse examined. In addition, twomore »« less
https://doi.org/10.1021/ac100954p
Online, Absolute Quantitation of Propranolol from Spatially Distinct 20- and 40-μm Dissections of Brain, Liver, and Kidney Thin Tissue Sections by Laser Microdissection–Liquid Vortex Capture–Mass Spectrometry

Journal Article Kertesz, Vilmos ; Vavrek, Marissa ; Freddo, Carol ; ... - Analytical Chemistry

Here, spatial resolved quantitation of chemical species in thin tissue sections by mass spectrometric methods has been constrained by the need for matrix-matched standards or other arduous calibration protocols and procedures to mitigate matrix effects (e.g., spatially varying ionization suppression). Reported here is the use of laser cut and drop sampling with a laser microdissection-liquid vortex capture electrospray ionization tandem mass spectrometry (LMD-LVC/ESI-MS/MS) system for online and absolute quantitation of propranolol in mouse brain, kidney, and liver thin tissue sections of mice administered with the drug at a 7.5 mg/kg dose, intravenously. In this procedure either 20 μm x 20more »« less
Cited by 5
https://doi.org/10.1021/acs.analchem.6b01155

Full Text Available
Continuous Flow Liquid Microjunction Surface Sampling Probe Connected On-line with HPLC/MS for Spatially Resolved Analysis of Small Molecules and Proteins

Journal Article Van Berkel, Gary J ; Kertesz, Vilmos - Rapid Communications in Mass Spectrometry

RATIONALE: A continuous flow liquid microjunction surface sampling probe extracts soluble material from surfaces for direct ionization and detection by MS. Demonstrated here is the on-line coupling of such a probe with HPLC/MS enabling extraction, separation and detection of small molecules and proteins from surfaces in a spatially resolved (~0.5 mm diameter spots) manner. Methods: A continuous flow liquid microjunction surface sampling probe was connected to a 6-port, 2-position valve for extract collection and injection to an HPLC column. A QTRAP 5500 hybrid triple quadrupole linear ion trap equipped with a Turbo V ion source operated in positive ESI modemore »« less
https://doi.org/10.1002/rcm.6580
Automated Coupling of Nanodroplet Sample Preparation with Liquid Chromatography–Mass Spectrometry for High-Throughput Single-Cell Proteomics

Journal Article Williams, Sarah M. ; Liyu, Andrey V. ; Tsai, Chia-Feng ; ... - Analytical Chemistry

Single-cell proteomics can provide critical biological insight into the cellular heterogeneity that is masked by bulk-scale analysis. Here, we have developed a nanoPOTS (nanodroplet processing in one pot for trace samples) platform and demonstrated its broad applicability for single-cell proteomics. However, because of nanoliter-scale sample volumes, the nanoPOTS platform is not compatible with automated LC-MS systems, which significantly limits sample throughput and robustness. To address this challenge, we have developed a nanoPOTS autosampler allowing fully automated sample injection from nanowells to LC-MS systems. We also developed a sample drying, extraction, and loading workflow to enable reproducible and reliable sample injection.more »« less
https://doi.org/10.1021/acs.analchem.0c01551

Similar Records

Title: Combined Falling Drop/Open Port Sampling Interface System for Automated Flow Injection Mass Spectrometry

Abstract

Citation Formats

Solid phase microextraction coupled to mass spectrometry via a microfluidic open interface for rapid therapeutic drug monitoring journal, January 2019

Solid phase microextraction coupled to mass spectrometry via a microfluidic open interface for rapid therapeutic drug monitoring
journal, January 2019